CAJ | 학술논문

从福州永泰温泉分离到1株降解纤维素嗜热菌,通过形态特征和16S rDNA序列鉴定,将其命名为地芽孢杆菌(Geobacillus sp.)TC-S8.采用3,5-二硝基水杨酸显色法(DNS法)对菌株TC-S8产胞外纤维素酶的发酵条件及酶性质进行研究.在培养基的初始pH为6.5,温度为60 ℃,装液量20%,接种量2%,摇床转速为120 r·min~(-1)的条件下培养36 h,菌株TC-S8产酶量达到最大(229 mU·mL~(-1)).其分泌的纤维素酶最适作用温度为60-70 ℃,最适pH为7.0.纤维素酶降解产物主要为葡萄糖,并有少量纤维二糖.
종복주영태온천분리도1주강해섬유소기열균,통과형태특정화16S rDNA서렬감정,장기명명위지아포간균(Geobacillus sp.)TC-S8.채용3,5-이초기수양산현색법(DNS법)대균주TC-S8산포외섬유소매적발효조건급매성질진행연구.재배양기적초시pH위6.5,온도위60 ℃,장액량20%,접충량2%,요상전속위120 r·min~(-1)적조건하배양36 h,균주TC-S8산매량체도최대(229 mU·mL~(-1)).기분비적섬유소매최괄작용온도위60-70 ℃,최괄pH위7.0.섬유소매강해산물주요위포도당,병유소량섬유이당.
A cellulose-degradation thermophilic bacteria was isolated from hotsprings of Yongtai County in Fuzhou, which was named as Geobacillus sp.TC-S8 by identification of morphological characteristics and 16S rDNA sequence. Ferment condition and character of the extracellular cellulase of Geobacillus sp.TC-S8 were analyzed with 3,5-dinitrosalicylic acid. The enzyme yield of strain TC-S8 reached its peak (229 mU·mL~(-1)) after culturing 36 h at the conditions of initial pH of culture medium was 6.5, temperature was 60 ℃, broth content was 20%, inoculum was 2% and rotation speed was 120 r·min~(-1). The optimum temperature of the cellulase excreted by strain TC-S8 was 60-70 ℃ and the optimum pH was 7.0. The degradation products were mainly glucose and a little cellobiose.