CAJ | 학술논문

目的研究p21-activated kinase-1(PAK1)基因对大肠癌细胞系体外侵袭能力的影响.方法把重组p21活化蛋白激酶1质粒用脂质体转染大肠癌SW480细胞,同时设立空白对照组和空载体对照组.于转染后48 h采用免疫印迹方法检测PAK1的蛋白表达水平,Boyden小室模型检测大肠癌细胞SW480在转染重组PAK1基因质粒后侵袭能力的变化.结果 SW480细胞转染p21活化蛋白激酶1重组质粒后,与空白对照和空载体对照相比,PAK1蛋白水平明显增加,细胞的体外侵袭能力增强.结论转染pPAK1重组质粒能够有效上调PAK1基因,增强大肠癌细胞系体外侵袭潜能,提示PAK1基因高表达可能与大肠癌细胞的侵袭和转移等生物学行为相关.
목적 연구p21-activated kinase-1(PAK1)기인대대장암세포계체외침습능력적영향.방법 파중조p21활화단백격매1질립용지질체전염대장암SW480세포,동시설립공백대조조화공재체대조조.우전염후48 h채용면역인적방법검측PAK1적단백표체수평,Boyden소실모형검측대장암세포SW480재전염중조PAK1기인질립후침습능력적변화.결과 SW480세포전염p21활화단백격매1중조질립후,여공백대조화공재체대조상비,PAK1단백수평명현증가,세포적체외침습능력증강.결론 전염pPAK1중조질립능구유효상조PAK1기인,증강대장암세포계체외침습잠능,제시PAK1기인고표체가능여대장암세포적침습화전이등생물학행위상관.
Objective To observe the effect of p21-activated kinase-1(PAK1)gene transfection on the invasiveness of human colorectal carcinoma SW480 cells in vitro.Methods SW480 cells in routine culture were transfected with the recombinant plasmid EGFP-C1/PAK1 via Lipofectamine~(TM) 2000.The expression of PAK1 protein in SW480 cells was detected using Western blotting,and the changes of the invasiveness of SW480 cells were evaluated using Boyden chamber invasion assay.Results Forty-eight hours after transfection with pEGFP-C1/PAK1,the PAK1 protein expression increased significantly in comparison with those in negative and vector control groups.The invasiveness of the SW480 cells was significantly enhanced after the transfection. Conclusion The PAK1 gene transfection call increase the expression of PAK1 in SW480 cells and enhance the invasiveness of the cells.PAK1 can be associated with the invasiveness and metastasis of colorectal carcinoma cells.