猪屎豆丛枝病植原体的分子检测与鉴定
저시두총지병식원체적분자검측여감정
Molecular Identification of Crotalaria Witches'-Broom Phytoplasma
  • 万方数据
    林业科学 임업과학
    SCIENTIA SILVAE SINICAE
    2010年 1期 163-168 ,共6页

    李永%徐启聪%田国忠%郭民伟%朴春根

    리영%서계총%전국충%곽민위%박춘근

    猪屎豆丛枝病%植原体%分子鉴定%序列测定 豬屎豆叢枝病%植原體%分子鑒定%序列測定
    저시두총지병%식원체%분자감정%서렬측정
    Crotalaria witches'-broom phytoplasma%phytoplasma%molecular identification%sequencing


    Crotalaria witches '-broom phytoplasma (CrWB) collected from Hainan Province,was classified and identified by sequencing techniques and Virtual RFLP.Two different type universal primer pairs of phytoplasma were used to amplify a 1.8 kb fragment of 16S rDNA and a 1.3 kb fragment of rp gene.The PCR-amplified products were cloned and sequenced.The results of sequencing and homologuous comparison with other phytoplasmas showed that Crotalaria witches'-broom phytoplasma shared 99.9 % similarity with peanut witches '-broom phytoplasma in 16S rRNA gene and 99.9% in rp gene.Crotalaria witches '-broom phytoplasma is sorted into Peanut witches'-hroom group (16SrII),16SrII-A,according to the results of Virtual RFLP of 16S rDNA,phylogenetic analysis of 16S rRNA and rp gene,which is related to Ca.Phytoplasma australasiae.
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