CAJ | 학술논문

为了分析中国对虾(Fenneropenaeus chinensis)抗白斑综合征病毒(WSSV)相关的分子机理,本研究以中国对虾"黄海2号"作为实验材料,进行白斑综合征病毒感染实验,感染20 d后获得了59尾存活的对虾.采用mRNA差异显示技术(DDRT-PCR)分析感染实验中早期濒死中国对虾和感染20d后仍存活对虾基因表达的差异,共回收到46个阳性差异片段.差异片段序列同源性功能分析的结果表明,差异片段所编码的蛋白主要参与氧化磷酸化、蛋白质合成、糖酵解、信号转导等生物过程.以上结果初步反映了中国对虾抗白斑综合征病毒相关基因的表达特征,为研究对虾抗白斑综合征病毒的作用机制及培育抗白斑综合征病毒对虾新品种提供了基础数据.
위료분석중국대하(Fenneropenaeus chinensis)항백반종합정병독(WSSV)상관적분자궤리,본연구이중국대하"황해2호"작위실험재료,진행백반종합정병독감염실험,감염20 d후획득료59미존활적대하.채용mRNA차이현시기술(DDRT-PCR)분석감염실험중조기빈사중국대하화감염20d후잉존활대하기인표체적차이,공회수도46개양성차이편단.차이편단서렬동원성공능분석적결과표명,차이편단소편마적단백주요삼여양화린산화、단백질합성、당효해、신호전도등생물과정.이상결과초보반영료중국대하항백반종합정병독상관기인적표체특정,위연구대하항백반종합정병독적작용궤제급배육항백반종합정병독대하신품충제공료기출수거.
White spot syndrome is a viral disease that seriously threatens shrimp aquaculture. In order to study the molecular basis related to WSSV-resistance in Fenneropenaeus chinensis, "Huanghai No.2" was challenged by WSSV. There were 59 shrimps survived from the challenge experiment after twenty days post-infection in the end. The gene expression in survival shrimps twenty days post-infection was compared with the earliest moribund shrimps in post-infection by mRNA Differential Display PCR. Forty-six positive differential fragments were recovered. Then sequences of the positive differential fragments were determined and blasted in GenBank. Most of them were found to code proteins related to oxidative phosphorylation, protein synthesis, glycolysis and signal transduction. The study showed partly the molecular basis related to WSSV-resistant genes in shrimp, and provided basic data for understanding mechanisms related to WSSV-resistant in shrimp and WSSV-resistant shrimp breeding.