CAJ | 학술논문

[目的]分析大豆农艺性状的QTL,为探讨大豆的遗传机制及进行遗传育种提供参考.[方法]以栽培大豆"合丰25"为母本和半野牛大豆"新民6号"为父本杂交得到的122个F8代重组自交系为试材,应用复合区间作图法对蛋白质含量、脂肪含量、产量、百粒重、生育期5个数量性状进行QTL定位和遗传效应分析.蛋白质、脂肪含量均使用近红外谷物分析仪测定.[结果]控制蛋白质含量、脂肪含量、产量、百粒重,生育期性状的4、4、1、2、5个共16个QTL位点,遗传贡献率在7.4%～33.7%.其中,遗传贡献率较大的主效QTL有分别位于1连锁群上Sat562-sat_219、Sat_219-Satt496、Sat_219-Satt496区间的3个控制蛋白质含量的QTL位点,其遗传贡献率分别为29.15%、33.70%和31.67%,且均为来自母本合丰25的加效基因,还有位于0连锁群上Satt477-Satd31、Satd31-Satt153区间的2个控制生育期QTL位点,其遗传贡献率分别为24.69%和24.96%,也是来自母本合丰25的加效基因.另外,6个分别距M连锁群Satt175(蛋白质)、Al连锁群Satt684(油分)、F连锁群Satt348(油分)、J连锁群Sat_412(油分)、Cl连锁群Sat_416(百粒重)、Cl连锁群Sat_416(生育期)标记仪有0.01 cm的QTL位点.[结论]定位了,影响蛋白质含量、油分含量、产量、百粒重和生育期5个重要农艺性状的QIL位点.
[목적]분석대두농예성상적QTL,위탐토대두적유전궤제급진행유전육충제공삼고.[방법]이재배대두"합봉25"위모본화반야우대두"신민6호"위부본잡교득도적122개F8대중조자교계위시재,응용복합구간작도법대단백질함량、지방함량、산량、백립중、생육기5개수량성상진행QTL정위화유전효응분석.단백질、지방함량균사용근홍외곡물분석의측정.[결과]공제단백질함량、지방함량、산량、백립중,생육기성상적4、4、1、2、5개공16개QTL위점,유전공헌솔재7.4%～33.7%.기중,유전공헌솔교대적주효QTL유분별위우1련쇄군상Sat562-sat_219、Sat_219-Satt496、Sat_219-Satt496구간적3개공제단백질함량적QTL위점,기유전공헌솔분별위29.15%、33.70%화31.67%,차균위래자모본합봉25적가효기인,환유위우0련쇄군상Satt477-Satd31、Satd31-Satt153구간적2개공제생육기QTL위점,기유전공헌솔분별위24.69%화24.96%,야시래자모본합봉25적가효기인.령외,6개분별거M련쇄군Satt175(단백질)、Al련쇄군Satt684(유분)、F련쇄군Satt348(유분)、J련쇄군Sat_412(유분)、Cl련쇄군Sat_416(백립중)、Cl련쇄군Sat_416(생육기)표기의유0.01 cm적QTL위점.[결론]정위료,영향단백질함량、유분함량、산량、백립중화생육기5개중요농예성상적QIL위점.
[Objective]The aim was to analyze QTL of agronomic traits in soybean and provide reference for a discussion on soybean genetic mechanism and genetic breeding.[Method]The composite interval mapping method was used for QTL location and genetic effects analysis on 5 quantitative traits including protein content,fat content,yield,100-grain weight and growth period.[Result]The control of these traits 4,4,1,2,5,a total of 16 QTL loci was detected.The genetic contribution rate was in 7.4%-33.7%,among which,a large main-effect QTL of the genetic contribution rate were located in linkage group Ⅰ Satt562-Sat_219,Sat-219-Satt496,Set219-Satt496 interval of the three control protein content QTL.sites,their genetic contribution rates were 29.15%,33.7 % and 31.67% respectively,all from the female parent Hefeng 25 plus minor gene;still in O linkage group Satt477-Satt331,Satt331-Satt153 interval of two control growing peried QTL loci,their genetic contribution rates were up to 24.69% and 24.96%,also from the female parent Hefeng 25 plus minor gene.In addition,six QTL sites from M linkage group Satt175 (protein),A1 linkage group Satt684 (oil),F linkage group Satt348 (oil),J linkage group Sat_4f2 (oil),C1 linkage group Sat_416 (100-grain weight) and C1 linkage group Sat_4t6 (growth period) marks only 0.01 cm were detected.[Conclusion]QTL sites which had effects on the 5 important agronomic traits in soybean were located.