中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2009年
9期
544-548
,共5页
徐幽琼%郑能雄%李宝华%张文昌%林新国%汪家梨%陈国兴
徐幽瓊%鄭能雄%李寶華%張文昌%林新國%汪傢梨%陳國興
서유경%정능웅%리보화%장문창%림신국%왕가리%진국흥
电磁场%动情期%性激素类%卵泡闭锁
電磁場%動情期%性激素類%卵泡閉鎖
전자장%동정기%성격소류%란포폐쇄
Eletromagnetic fields%Estrus%Sex hormones%Follicular atresia
目的 研究亚慢性暴露高频电磁场(HF-EMF,30 MHz)对雌性大鼠性腺的毒性作用.方法 选用清洁级Wistar雌性大鼠60只,按体重随机分为5组,暴露剂量分别为0、25、100、400、1600V/m,大鼠伞身暴露于HF-EMF,8 h/d,每周连续5 d,停2 d,共暴露56 d.辐射期间大鼠每3天称1次体重,第48天开始观察大鼠动情周期的变化,56 d后待大鼠处于动情期时取血清和卵巢,采用放射免疫法测定相火激素,HE染色法计算卵巢各级卵泡的数目构成比,用电子显微镜观察细胞超微结构.结果 各剂量组大鼠体重与卵巢湿重及脏器系数的差异无统计学意义(P0.05);100、400、1600V/m组大鼠动情前期时间分别为(15.00±5.06)、(11.40±2.05)、(10.56±0.96)h,明显短于对照组[(18.70±2.96)h],差异有统计学意义(P<0.01);400、1600 V/m组大鼠动情问期时间分别为(101.20±17.81)、(115.33±19.28)h,明显长于对照组[(69.80±11.42)h],差异有统计学意义(P<0.01).400、1600V/m组的黄体生成素(LH)水平[分别为(11.02±1.11)、(14.70±1.94)mU/ml]明显高于对照组[(8.70±0.53)mU/ml],差异有统计学意义(P<0.01);而雌激素(E2)水平[分别为(57.16±31.56)、(50.57±25.16)pg.ml]明显低于对照组[(95.04±32.62)pg/ml],差异有统计学意义(P<0.01);400、1600V/m组黄体数目构成比(分别为19.75%、19.04%)明显高于对照组(14.01%),差异有统计学意义(P<0.01),100、400、1600V/m组闭锁卵泡数目构成比分别为8.45%、9.95%、11.70%,明显高于对照组(7.72%),差异有统计学意义(P<0.01);而400、1600 V/m组成熟卵泡数目构成比(分别为1.50%、1.55%)明显低于对照组(3.36%),差异有统汁学意义(P<0.01);400、1600 V/m组仞/次级卵泡数目构成比分别为22.24%、21.09%,明显低于对照组(26.60%),差异有统计学意义(P<0.01).随辐射剂量的增大,细胞超微结构改变越明显,细胞核染色质成块,核仁边集,甚至出现核同缩、凋亡小体,线粒体出现肿胀、水肿,甚至呈空泡状.结论 HF-EMF(30 MHz、100～1600 V/m)对雌性性腺有毒性作用,其毒性作用表现在卵巢损害出现较早(如激素分泌功能改变).
目的 研究亞慢性暴露高頻電磁場(HF-EMF,30 MHz)對雌性大鼠性腺的毒性作用.方法 選用清潔級Wistar雌性大鼠60隻,按體重隨機分為5組,暴露劑量分彆為0、25、100、400、1600V/m,大鼠傘身暴露于HF-EMF,8 h/d,每週連續5 d,停2 d,共暴露56 d.輻射期間大鼠每3天稱1次體重,第48天開始觀察大鼠動情週期的變化,56 d後待大鼠處于動情期時取血清和卵巢,採用放射免疫法測定相火激素,HE染色法計算卵巢各級卵泡的數目構成比,用電子顯微鏡觀察細胞超微結構.結果 各劑量組大鼠體重與卵巢濕重及髒器繫數的差異無統計學意義(P0.05);100、400、1600V/m組大鼠動情前期時間分彆為(15.00±5.06)、(11.40±2.05)、(10.56±0.96)h,明顯短于對照組[(18.70±2.96)h],差異有統計學意義(P<0.01);400、1600 V/m組大鼠動情問期時間分彆為(101.20±17.81)、(115.33±19.28)h,明顯長于對照組[(69.80±11.42)h],差異有統計學意義(P<0.01).400、1600V/m組的黃體生成素(LH)水平[分彆為(11.02±1.11)、(14.70±1.94)mU/ml]明顯高于對照組[(8.70±0.53)mU/ml],差異有統計學意義(P<0.01);而雌激素(E2)水平[分彆為(57.16±31.56)、(50.57±25.16)pg.ml]明顯低于對照組[(95.04±32.62)pg/ml],差異有統計學意義(P<0.01);400、1600V/m組黃體數目構成比(分彆為19.75%、19.04%)明顯高于對照組(14.01%),差異有統計學意義(P<0.01),100、400、1600V/m組閉鎖卵泡數目構成比分彆為8.45%、9.95%、11.70%,明顯高于對照組(7.72%),差異有統計學意義(P<0.01);而400、1600 V/m組成熟卵泡數目構成比(分彆為1.50%、1.55%)明顯低于對照組(3.36%),差異有統汁學意義(P<0.01);400、1600 V/m組仞/次級卵泡數目構成比分彆為22.24%、21.09%,明顯低于對照組(26.60%),差異有統計學意義(P<0.01).隨輻射劑量的增大,細胞超微結構改變越明顯,細胞覈染色質成塊,覈仁邊集,甚至齣現覈同縮、凋亡小體,線粒體齣現腫脹、水腫,甚至呈空泡狀.結論 HF-EMF(30 MHz、100～1600 V/m)對雌性性腺有毒性作用,其毒性作用錶現在卵巢損害齣現較早(如激素分泌功能改變).
목적 연구아만성폭로고빈전자장(HF-EMF,30 MHz)대자성대서성선적독성작용.방법 선용청길급Wistar자성대서60지,안체중수궤분위5조,폭로제량분별위0、25、100、400、1600V/m,대서산신폭로우HF-EMF,8 h/d,매주련속5 d,정2 d,공폭로56 d.복사기간대서매3천칭1차체중,제48천개시관찰대서동정주기적변화,56 d후대대서처우동정기시취혈청화란소,채용방사면역법측정상화격소,HE염색법계산란소각급란포적수목구성비,용전자현미경관찰세포초미결구.결과 각제량조대서체중여란소습중급장기계수적차이무통계학의의(P0.05);100、400、1600V/m조대서동정전기시간분별위(15.00±5.06)、(11.40±2.05)、(10.56±0.96)h,명현단우대조조[(18.70±2.96)h],차이유통계학의의(P<0.01);400、1600 V/m조대서동정문기시간분별위(101.20±17.81)、(115.33±19.28)h,명현장우대조조[(69.80±11.42)h],차이유통계학의의(P<0.01).400、1600V/m조적황체생성소(LH)수평[분별위(11.02±1.11)、(14.70±1.94)mU/ml]명현고우대조조[(8.70±0.53)mU/ml],차이유통계학의의(P<0.01);이자격소(E2)수평[분별위(57.16±31.56)、(50.57±25.16)pg.ml]명현저우대조조[(95.04±32.62)pg/ml],차이유통계학의의(P<0.01);400、1600V/m조황체수목구성비(분별위19.75%、19.04%)명현고우대조조(14.01%),차이유통계학의의(P<0.01),100、400、1600V/m조폐쇄란포수목구성비분별위8.45%、9.95%、11.70%,명현고우대조조(7.72%),차이유통계학의의(P<0.01);이400、1600 V/m조성숙란포수목구성비(분별위1.50%、1.55%)명현저우대조조(3.36%),차이유통즙학의의(P<0.01);400、1600 V/m조인/차급란포수목구성비분별위22.24%、21.09%,명현저우대조조(26.60%),차이유통계학의의(P<0.01).수복사제량적증대,세포초미결구개변월명현,세포핵염색질성괴,핵인변집,심지출현핵동축、조망소체,선립체출현종창、수종,심지정공포상.결론 HF-EMF(30 MHz、100～1600 V/m)대자성성선유독성작용,기독성작용표현재란소손해출현교조(여격소분비공능개변).
Objective To study on the effects of sub-chronic exposures to high-frequency electromag-netic field (HF-EMF) on the estrous cycle, ovarian pathological changes and related hormones and preliminari-ly investigate the female genital toxicities of HF-EMF in rats. Methods 60 Wistar female adult rats were randomly divided into five groups based on body weight and radiated with different levels of 30 MHz electro-magnetic field (0, 25, 100, 400 and 1600 V/m) for eight hours daily. Weekly the rats were continuously ex-posed five days. From the 48th day the four stage of estrus cycle were observed with smear method of the vagi-nal cell. Fifty-six days later the serum levels of sexual hormones were detected with the radioimmunoassay on estrus stage. The constituent ratio of the distinct follicle number on ovaries were observed with the HE staining and the uhramicrostrueture was observed with the transmission electron microscope. Meanwhile, the ovarial humid weight and organ coefficient were observed. Results There was no significant difference in ovarial humid weight and organ coefficient between the exposure and the control groups. The time of preoestrus in the 100 V/m group, the 400 V/m group and the 1600 V/m group was decreased significantly [(15.00±5.06), ( 11.40±2.05 ) and (10.56±0.96) h in the exposure group compared with (18.70±2.96) h in the control group, P<0.01], and the time of Anestrum in the 400 V/m group and the 1600 V/m group were increased significantly [(101.20±17.81 ) and (115.33±19.28) h in the exposure group compared with (69.80±11.42) h in the control group, P<0.01 )]. Serum LH in the 400 V/m and 1600 V/m group was increased significantly[( 11.02±1.11 ) and ( 14.70±1.94) mU/ml in the exposure groups compared with (8.70±0.53) mU/ml in the control group, P<0.01 ], and serum E2 was decreased significantly [(57.16±31.56) and (50.57±25.16) pg/ml in the exposure groups compared with (95.04±32.62) pg/ml in the control group, P<0.01]. The composition ratio of the corpus luteum/ albicans number in the 400 V/m group and the 1600 V/m group was increased significandy (19.75% and 19.04% in the exposure groups compared with 14.01% in the control group, P<0.05 ). The composition ratio of the atresic follicle number was increased significantly in the 100 V/m, the 400 V/m and the 1600 V/m group (8.45%, 9.95% and 11.70% in the exposure groups compared with 7.72% in the control group, P<0.01 ).The composition ratio of the mature follicle and the pri/sec follicle was decreased significantly in the 400 V/m and the 1600 V/m group (1.50% and 1.55% in the exposure groups compared with 3.36% in the control group.22.24% and 21.09% in the exposure groups compared with 26.60% in the control group, P<0.01 ). Along with the increase of radiation dose, the ultramicrostructure of cell on the ovaries appeared more abnormal.Conclusions The toxicities of female gonads are closely associated with exposures to HF-EMF. The nonage damage of female gonadal toxicities might emerge on the ovaries.
