国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2011年
2期
134-137
,共4页
戴文伟%许绍强%孙卫文%邓维意%伍建伟%李明昌%何伟文%陆永健%陈盛强
戴文偉%許紹彊%孫衛文%鄧維意%伍建偉%李明昌%何偉文%陸永健%陳盛彊
대문위%허소강%손위문%산유의%오건위%리명창%하위문%륙영건%진성강
同型半胱氨酸%颅内动脉瘤%N5,10-亚甲基四氢叶酸还原酶%多态性
同型半胱氨痠%顱內動脈瘤%N5,10-亞甲基四氫葉痠還原酶%多態性
동형반광안산%로내동맥류%N5,10-아갑기사경협산환원매%다태성
Homocysteine%Methylenetetrahydrofolate reductase%Intracranial aneurysm%Polymorphism
目的 探讨同型半胱氨酸(Hcy)及其代谢关键酶N5,10-亚甲基四氢叶酸还原酶(MTHFR)C677T基因多态性与颅内动脉瘤的关系.方法 采用荧光偏振免疫法检测76例颅内动脉瘤患者和77例健康成人的血浆Hcy水平,并利用聚合酶链式反应技术检测其MTHFR C677T基因多态性.结果 颅内动脉瘤组血浆Hcy水平(16.14±6.72)μ mol/L高于对照组(11.20±3.20)μmol/L,两组间的差异有显著性(P<0.05).两组MTHFR C677T等位基因频率差异无显著性(P>0.05);颅内动脉瘤组MTHFR C677T CT基因型频率明显高于正常对照组(P<0.01),患颅内动脉瘤的风险是对照组的2.57倍.CC、CT基因型颅内动脉瘤组患者血浆Hcy水平明显高于对照组(P<0.01);颅内动脉瘤组患者CT基因型的血浆Hcy浓度较其它基因型高(P<0.05).结论 高Hcy血症与颅内动脉瘤病发生有一定关系,MTHFR CT基因型与颅内动脉瘤明显相关.
目的 探討同型半胱氨痠(Hcy)及其代謝關鍵酶N5,10-亞甲基四氫葉痠還原酶(MTHFR)C677T基因多態性與顱內動脈瘤的關繫.方法 採用熒光偏振免疫法檢測76例顱內動脈瘤患者和77例健康成人的血漿Hcy水平,併利用聚閤酶鏈式反應技術檢測其MTHFR C677T基因多態性.結果 顱內動脈瘤組血漿Hcy水平(16.14±6.72)μ mol/L高于對照組(11.20±3.20)μmol/L,兩組間的差異有顯著性(P<0.05).兩組MTHFR C677T等位基因頻率差異無顯著性(P>0.05);顱內動脈瘤組MTHFR C677T CT基因型頻率明顯高于正常對照組(P<0.01),患顱內動脈瘤的風險是對照組的2.57倍.CC、CT基因型顱內動脈瘤組患者血漿Hcy水平明顯高于對照組(P<0.01);顱內動脈瘤組患者CT基因型的血漿Hcy濃度較其它基因型高(P<0.05).結論 高Hcy血癥與顱內動脈瘤病髮生有一定關繫,MTHFR CT基因型與顱內動脈瘤明顯相關.
목적 탐토동형반광안산(Hcy)급기대사관건매N5,10-아갑기사경협산환원매(MTHFR)C677T기인다태성여로내동맥류적관계.방법 채용형광편진면역법검측76례로내동맥류환자화77례건강성인적혈장Hcy수평,병이용취합매련식반응기술검측기MTHFR C677T기인다태성.결과 로내동맥류조혈장Hcy수평(16.14±6.72)μ mol/L고우대조조(11.20±3.20)μmol/L,량조간적차이유현저성(P<0.05).량조MTHFR C677T등위기인빈솔차이무현저성(P>0.05);로내동맥류조MTHFR C677T CT기인형빈솔명현고우정상대조조(P<0.01),환로내동맥류적풍험시대조조적2.57배.CC、CT기인형로내동맥류조환자혈장Hcy수평명현고우대조조(P<0.01);로내동맥류조환자CT기인형적혈장Hcy농도교기타기인형고(P<0.05).결론 고Hcy혈증여로내동맥류병발생유일정관계,MTHFR CT기인형여로내동맥류명현상관.
0bjective To explore relationship between plasma homocysteine(Hcy) level and polymorphism of methylenetetrahydrofolate reductase gene(MTHFR) with intracranial aneurysm.Methods The genotyes of MTHFR were determined by PCR-based assay and their plasma Hcy levels were determined in 76 patients with intracranial aneurysm and 77 healthy controls. Results There were significant differences in the CT genotype frequenceies of MTHFR mutations between the patients and central groups(P＜0.01). Mean total plasma Hcy concentrations were significantly higher in intracranial aneurysm patients than those in the normal subjects. Conclusion Hyperhomocysteinemia may be independent risk factors for intracranial aneurysm and MTHFR CT genotype may be independent risk factors for intracranial aneurysm.
