CAJ | 학술논문

目的探讨环孢素A(CsA)对人类骨髓间充质干细胞(MSC)增殖活性的影响,为研究CsA对骨髓增牛异常综合征(MDS)患者骨髓MSC增殖的影响提供依据.方法采用四甲基偶氮唑盐(MTT)微量酶反应比色法,经2.5×10~4、2.5×10~3、2.5×10~2、2.5×10 ng/μl CsA刺激后检测对照组和MDS患者骨髓MSC的吸光度(A值).结果 CsA不同质量浓度间骨髓MSC增殖效应差异无统计学意义(P>0.05);在(2.5×10～2.5×10~4)ng/μl质量浓度范围内,未观察到CsA有细胞毒性作用;MDS患者骨髓MSC增殖效应高于健康对照(P<0.01).CsA质量浓度与组别问交瓦作用差异无统计学意义(P>0.05).不同质量浓度CsA处理组与对照组骨髓MSC增殖效应差异无统计学意义.结论 CsA对MDS患者骨髓MSC和健康患者MSC增殖影响不明显.
목적 탐토배포소A(CsA)대인류골수간충질간세포(MSC)증식활성적영향,위연구CsA대골수증우이상종합정(MDS)환자골수MSC증식적영향제공의거.방법 채용사갑기우담서염(MTT)미량매반응비색법,경2.5×10~4、2.5×10~3、2.5×10~2、2.5×10 ng/μl CsA자격후검측대조조화MDS환자골수MSC적흡광도(A치).결과 CsA불동질량농도간골수MSC증식효응차이무통계학의의(P>0.05);재(2.5×10～2.5×10~4)ng/μl질량농도범위내,미관찰도CsA유세포독성작용;MDS환자골수MSC증식효응고우건강대조(P<0.01).CsA질량농도여조별문교와작용차이무통계학의의(P>0.05).불동질량농도CsA처리조여대조조골수MSC증식효응차이무통계학의의.결론 CsA대MDS환자골수MSC화건강환자MSC증식영향불명현.
Objective To provide data for reference on the impact of cyclosporin A (CsA) on the proliferation of the bone marrow mesenchymal stem cells in MDS patients through the investigation of the impact of cyclosporin A on human bone marrow mesenchymal stem cell proliferation. Methods The absorption rates of the bone marrow mesenchymal stem cells in the control group and the MDS patient group were determined by using the tetrazolinm salt (MTT) micro-colorimetric enzyme reaction. The concentrations of cyclosporine A are 2.5×10~4 ng/μl, 2.5×10~3 ng/μl, 2.5×10~2 ng/μl and 2.5×10ng/μl respectively. Results There is no significant difference between the each result by using different concentrations of CsA., which indicates the impact of CsA on the growth of mesenchymal stem cells is not significant(P >0.05). In the serial of concentrations mentioned, no cytotoxicity of CsA is observed. However, there is difference between the selected indicators of the control group and the patient group (P <0.01), and the value of the MDS patient group is higher than that of the control group. There is no statistic difference between the concentration of CsA and the data obtained from interactions between different groups (P >0.05). There is no significant difference between the absorption rates of the group treated by CsA of every concentration and the corresponding control group. Conclusion The impact of CsA on the bone marrow mesenchymal stem cell proliferation is significant in neither healthy people nor MDS patients.