CAJ | 학술논문

目的探讨茶氨酸通过树突状细胞(DCs)调节T淋巴细胞抗肺癌A549的影响,观察其对SCID小鼠移植瘤的免疫保护和治疗作用.方法 SCID小鼠免疫后,接种A549细胞株,以茶氨酸为受试因素刺激DC,观察其免疫保护作用和免疫治疗作用.结果肿瘤免疫保护方面,茶氨酸刺激DC组,以及DC组肿瘤形成时间明显延长(F=29.5,P＜0.01),且移植瘤在茶氨酸刺激DC组体积最小(F=69.51,P＜0.01),重量最轻(F=190.9,P＜0.01).在抗肿瘤治疗方面,其肿瘤比其他对照组更小,其表面光滑,无粘连,光镜下茶氨酸刺激DC组可见大薄片状坏死;茶氨酸刺激后的DC组移植瘤中VEGF的表达水平显著下降(F=31.4,P＜0.01).结论茶氨酸刺激后DC的抗肿瘤免疫保护和治疗机制,可能是通过茶氨酸刺激DC调节T细胞实现的,也可能与茶氨酸通过DC介导VEGF蛋白表达的下调,从而与抑制肿瘤血管形成有关.
목적 탐토다안산통과수돌상세포(DCs)조절T림파세포항폐암A549적영향,관찰기대SCID소서이식류적면역보호화치료작용.방법 SCID소서면역후,접충A549세포주,이다안산위수시인소자격DC,관찰기면역보호작용화면역치료작용.결과 종류면역보호방면,다안산자격DC조,이급DC조종류형성시간명현연장(F=29.5,P＜0.01),차이식류재다안산자격DC조체적최소(F=69.51,P＜0.01),중량최경(F=190.9,P＜0.01).재항종류치료방면,기종류비기타대조조경소,기표면광활,무점련,광경하다안산자격DC조가견대박편상배사;다안산자격후적DC조이식류중VEGF적표체수평현저하강(F=31.4,P＜0.01).결론 다안산자격후DC적항종류면역보호화치료궤제,가능시통과다안산자격DC조절T세포실현적,야가능여다안산통과DC개도VEGF단백표체적하조,종이여억제종류혈관형성유관.
Objective To reveal the impact of anti-lung cancer A549 by theanine, which may regulate T lymphocytes through dendritic cells (DCs), and to observe the immune protection of theanine on SCID mice transplanted tumors. Methods SCID mice were reconstructed after immunization, then A549 cells were inoculated. The immune protection and immune treatment of theanine stimulated DCs were observed. Results In the theanine-stimulated DC group, as well as DC group, the time needed to form tumor were significantly prolonged (F = 29. 5, P ＜ 0. 01). And the transplanted tumors were smallest (F =69. 51, P ＜0. 01) and lightest (F = 190. 9, P ＜0. 01) in the theanine-stimulated DC group. 50 days after tumor-bearing, the transplanted tumors in the theanine-stimulated DC group were smaller than in the control group, and the surface were smoother with no adhesions. Under light microscope, a large thin slice necrosis was showed in the theanine-stimulated DC group, and the VEGF expression was also reduced (F = 31.4, P ＜ 0. 01). Conclusions The anti-tumor immune protection and treatment mechanism of theanine-stimulated DCs were possibly through regulation of T cells, as well as the VEGF expression reducing,thereby inhibited tumor blood vessel formation.