中国实用眼科杂志
中國實用眼科雜誌
중국실용안과잡지
CHINESE JOURNAL OF PRACTICAL OPHTHALMOLOGY
2010年
2期
126-129
,共4页
陈兹满%邱波%袁进%陈家祺
陳玆滿%邱波%袁進%陳傢祺
진자만%구파%원진%진가기
角膜移植术,穿透性%泪液%白细胞介素-6%白细胞介素-10%荧光定量PCR
角膜移植術,穿透性%淚液%白細胞介素-6%白細胞介素-10%熒光定量PCR
각막이식술,천투성%루액%백세포개소-6%백세포개소-10%형광정량PCR
Penetrating keratoplasty%Tear%Interleukin-6%Interleukin- 10%Fluorescence quantitative
目的 追踪穿透性角膜移植术患者泪液中IL-6、IL-10mRNA动态表达规律,探讨炎症因子在角膜移植创伤愈合中的作用机制.方法 2007年7月至2007年12月接受穿透性角膜移植手术的患者20例为研究对象,吸取术前1d、术后1周及3周的泪液,利用实时荧光定量PCR技术检测IL-6、IL-10mRNA表达水平,20名健康人作为对照.结果 手术组病例患者术前1d、术后1周及3周泪液中IL-6的CT值分别为18.471±2.698、18.723±2.640、20.706±2.445,对照组泪液中IL-6的CT值为22.801±2.834.IL-10在相应的CT值检测点分别为15.178±1.640、16.042±2.516、16.690±2.296,对照组IL-10的CT值为21.251±3.111.经统计学t检验分析证实手术组患者术眼泪液中IL-6和IL-10的CT值含量在各个检测时间点均低于对照组(P均<0.01),术后3周患者术眼泪液中IL-6及IL-10的CT值明显高于术后早期检测值(P均<0.01).说明PKP术后患者泪液中炎症因子的表达强度呈下降趋势.结论 穿透性角膜移植手术患者泪液中炎症反应高于正常人,IL-6和IL-10可作为评价术后炎症反应强烈程度的检测指标,指导术后抗炎药物的规范使用.
目的 追蹤穿透性角膜移植術患者淚液中IL-6、IL-10mRNA動態錶達規律,探討炎癥因子在角膜移植創傷愈閤中的作用機製.方法 2007年7月至2007年12月接受穿透性角膜移植手術的患者20例為研究對象,吸取術前1d、術後1週及3週的淚液,利用實時熒光定量PCR技術檢測IL-6、IL-10mRNA錶達水平,20名健康人作為對照.結果 手術組病例患者術前1d、術後1週及3週淚液中IL-6的CT值分彆為18.471±2.698、18.723±2.640、20.706±2.445,對照組淚液中IL-6的CT值為22.801±2.834.IL-10在相應的CT值檢測點分彆為15.178±1.640、16.042±2.516、16.690±2.296,對照組IL-10的CT值為21.251±3.111.經統計學t檢驗分析證實手術組患者術眼淚液中IL-6和IL-10的CT值含量在各箇檢測時間點均低于對照組(P均<0.01),術後3週患者術眼淚液中IL-6及IL-10的CT值明顯高于術後早期檢測值(P均<0.01).說明PKP術後患者淚液中炎癥因子的錶達彊度呈下降趨勢.結論 穿透性角膜移植手術患者淚液中炎癥反應高于正常人,IL-6和IL-10可作為評價術後炎癥反應彊烈程度的檢測指標,指導術後抗炎藥物的規範使用.
목적 추종천투성각막이식술환자루액중IL-6、IL-10mRNA동태표체규률,탐토염증인자재각막이식창상유합중적작용궤제.방법 2007년7월지2007년12월접수천투성각막이식수술적환자20례위연구대상,흡취술전1d、술후1주급3주적루액,이용실시형광정량PCR기술검측IL-6、IL-10mRNA표체수평,20명건강인작위대조.결과 수술조병례환자술전1d、술후1주급3주루액중IL-6적CT치분별위18.471±2.698、18.723±2.640、20.706±2.445,대조조루액중IL-6적CT치위22.801±2.834.IL-10재상응적CT치검측점분별위15.178±1.640、16.042±2.516、16.690±2.296,대조조IL-10적CT치위21.251±3.111.경통계학t검험분석증실수술조환자술안루액중IL-6화IL-10적CT치함량재각개검측시간점균저우대조조(P균<0.01),술후3주환자술안루액중IL-6급IL-10적CT치명현고우술후조기검측치(P균<0.01).설명PKP술후환자루액중염증인자적표체강도정하강추세.결론 천투성각막이식수술환자루액중염증반응고우정상인,IL-6화IL-10가작위평개술후염증반응강렬정도적검측지표,지도술후항염약물적규범사용.
Objective To explore the sequencial changes of tear IL-6 mRNA and IL-10 mRNA in patients of penetrating keratoplasty (PKP) surgery, and to understand the the inflammatory response before and after PKP surgery and wound healing mechanism. Methods Selected from July 2007 to December 2007 PKP surgery patients with 20 healthy subjects as a control group. Tear was collected 1d before surgery, and 1w, 3w after surgery, fluorescence quantitative PCR was used to detect IL-6 mRNA and IL-10 mRNA in tear. Results The CT value of IL-6 at pre-operation, 1w post-operation and 3w post-operation was 18.471±2.698, 18.723±2.640 and 20.706±2.445, respectively, and that of the control was 22.801±2.834. The CT value of IL-10 at the same time was 15.178±1.640, 16.042±2.516 and 16.690±2.296, respoctively, that of control was 21.251±3.111. It was indicated that the content of IL-6 and IL-10 in tear in PKP surgery patients was higher than that in control (P<0.01), the content of IL-6 and IL-10 in tear trended to descend after surgery, compared with the level of early post-operation, the level of IL-6 and IL-10 in tear at 3w post-operation was decreased significantly (P<0.01). Conclusions The level of inflammatory factors in tear in patients of PKP surgery was higher than that of heatth human. IL-6 and IL-10 could be acted as markers of inflammatory reaction afer PKP surgery, and they could guide the rational usage of anti-inflammatory drugs after the surgery.
