CAJ | 학술논문

目的研究干燥综合征(SS)患者及健康志愿者外周血单个核细胞(PBMC) THRA,TCL1基因的差异表达,为探讨疾病的发病机制、诊断及治疗提供新的依据.方法选取40例原发性干燥综合征(pSS)患者,40名同年龄健康志愿者为研究对象,Ficoll密度梯度离心法分离PBMC,Trizol一步法提取mRNA,反转录至cDNA,实时定量聚合酶链反应(PCR)法检测PBMC中THRA,TCL1等基因的表达.采用秩和检验和t检验进行统计分析.结果 SS患者PBMC中TCL1的mRNA表达(2.5±4.7)明显高于健康对照,二者之间差异有统计学意义(Z=-2.045,P＜0.05)；而THRA的表达水平分别为(1 2±0.8)差异无统计学意义.淋巴结病变活动指数2～3分的SS患者TCLI表达水平(4.4±3.8)明显高于淋巴结病变活动指数1～2分的患者(11±11),二者之间差异有统计学意义(t=-2.319,P＜0.05),合并及未合并甲状腺疾病的SS患者THRA表达水平差异无统计学意义.结论 SS患者TCL1基因的表达明显高于健康对照,且与SS淋巴结病变活动指数有关,为进一步验证为疾病的发病机制,评估疾病的活动性及诊治提供了新的研究思路.
목적 연구간조종합정(SS)환자급건강지원자외주혈단개핵세포(PBMC) THRA,TCL1기인적차이표체,위탐토질병적발병궤제、진단급치료제공신적의거.방법 선취40례원발성간조종합정(pSS)환자,40명동년령건강지원자위연구대상,Ficoll밀도제도리심법분리PBMC,Trizol일보법제취mRNA,반전록지cDNA,실시정량취합매련반응(PCR)법검측PBMC중THRA,TCL1등기인적표체.채용질화검험화t검험진행통계분석.결과 SS환자PBMC중TCL1적mRNA표체(2.5±4.7)명현고우건강대조,이자지간차이유통계학의의(Z=-2.045,P＜0.05)；이THRA적표체수평분별위(1 2±0.8)차이무통계학의의.림파결병변활동지수2～3분적SS환자TCLI표체수평(4.4±3.8)명현고우림파결병변활동지수1～2분적환자(11±11),이자지간차이유통계학의의(t=-2.319,P＜0.05),합병급미합병갑상선질병적SS환자THRA표체수평차이무통계학의의.결론 SS환자TCL1기인적표체명현고우건강대조,차여SS림파결병변활동지수유관,위진일보험증위질병적발병궤제,평고질병적활동성급진치제공료신적연구사로.
Objective To identify novel markers such as THRA or TCL1 in Sj(o)gren's syndrome (SS) patients to discriminate them from hcalthy volunteers.Methods Experimental group (n=40) and healthy volunteer group (n=40) were recruited based on strict inclusion and exclusion criteria.Peripheral blood samples (9 ml) were collected and peripheral blood mononuclear cells were separated by Ficoll gradient centrifugation.RNA was extracted using Trizol reagent,and the expression of THRA,TCL1 in PBMCs was detected by real-time PCR.And the data were processed with Wilcoxon test and t test (P＜0.05).Results TCL1 and THRA expression level are higher in SS patients (2.5±4.7) than healthy volunteers (Z=-2.045,P＜0.05).The TCLI expression level (4.4±3.8) is higher in high lymphadenopathy activation index patients (2 to 3grade) than that in low lymphadenopathy activation index patients (grade 0 to 1 ) (t=-2.319,P＜0.05 ).Conclusion TCL1 expression level is higher in SS patients,and TCL1 expression level is related to the severity of lymphadenopathy,which provide a new platform of the study for the pathogenesis,disease severity evaluation,even dia-gnosis and treatment of SS.