CAJ | 학술논문

目的探讨壳聚糖微球转染人IL-1Ra与TGF-β1基因治疗兔膝关节早期骨关节炎的方法与效果.方法制备分别包裹IL-1Ra质粒DNA和TGF-β1质粒DNA的壳聚糖微球缓释系统,分组向兔膝关节早期骨关节炎模型关节腔注射含IL-1Ra基因和(或)TGF-β1基因的壳聚糖微球、不含上述基因的壳聚糖溶液,定期处死,使用ELISA检测关节腔灌洗液的IL-1Ra、TGF-β1浓度,取关节标本Mankin评分、苏木精-伊红(HE)染色、番红O染色及免疫组化检测.结果经过60 d观察,壳聚糖微球转染基因组的IL-1Ra与TGF-β1的基因可持续表达,双基因组的软骨标本从外观、染色观察,损伤程度轻于单基因组.双基因组的Mankin评分明显低于单基因组(P＜0.05),单基因组的Mankin评分明显低于空白组(P＜0.05).结论关节腔注射壳聚糖微球转染IL-1Ra与TGF-β1基因能抑制软骨的退变和促进软骨的修复.
목적 탐토각취당미구전염인IL-1Ra여TGF-β1기인치료토슬관절조기골관절염적방법여효과.방법 제비분별포과IL-1Ra질립DNA화TGF-β1질립DNA적각취당미구완석계통,분조향토슬관절조기골관절염모형관절강주사함IL-1Ra기인화(혹)TGF-β1기인적각취당미구、불함상술기인적각취당용액,정기처사,사용ELISA검측관절강관세액적IL-1Ra、TGF-β1농도,취관절표본Mankin평분、소목정-이홍(HE)염색、번홍O염색급면역조화검측.결과 경과60 d관찰,각취당미구전염기인조적IL-1Ra여TGF-β1적기인가지속표체,쌍기인조적연골표본종외관、염색관찰,손상정도경우단기인조.쌍기인조적Mankin평분명현저우단기인조(P＜0.05),단기인조적Mankin평분명현저우공백조(P＜0.05).결론 관절강주사각취당미구전염IL-1Ra여TGF-β1기인능억제연골적퇴변화촉진연골적수복.
Objective To explore the method and effect of transinfection of rabbit early knee osteoarthritis models via chitosan microsphere with gene of recombined human IL-1Ra gene and TGF-β1 gene. Methods Chitosan microspheres with plasmids of IL-1Ra gene and TGF-β1 gene, and rabbit early knee osteoarthritis models were prepared. Rabbits in different groups had intra-articular injections of chitosan microsphere containing IL-1Ra gene and / or TGF-β1 gene, and chitosan solution as control group before being executed regularly and randomly. The joint specimens were evaluated by HE staining, lycopene red O staining and immunohistochemical analysis and Mankin's score. ELISA was used for detection of IL-IRa and TGF-β1 concentration of articular cavity fluid in each group. Results The control group was consistent with the pathological changes of early OA. In co-transinfection group, judging from the appearance and staining of cartilage,the OA damage of the specimens was less serious than other groups'. Its Mankin's score was significantly lower than single-gene transinfection group (P ＜ 0.05), and the latters Mankin's score were significantly lower than control group (P ＜ 0.05). Conclusion Intra-articular injection of chitosan microspheres containing both IL-1Ra gene and TGF-β1 gene could inhibit the degeneration of cartilage and promote cartilage repair.