CAJ | 학술논문

目的通过比较瘢痕疙瘩与正常皮肤组织的蛋白质组表达差异,筛选出与瘢痕疙瘩产生相关的蛋白质.方法 2010年1月至6月运用蛋白质组学技术,对8例瘢痕疙瘩组织和3例正常皮肤组织进行差异双向凝胶电泳(2D-DIGE),选择差异蛋白质斑点,进行基质辅助激光解离飞行时间(MALDI-TOF/TOF)质谱分析和生物学信息分析.结果成功建立瘢痕疙瘩和正常皮肤组织的双向凝胶电泳图谱,瘢痕疙瘩和正常皮肤组织凝胶电泳图谱中平均蛋白质斑点数分别为2978和3053,其中表达差异超过4倍的斑点共有40个,质谱分析和数据库检索共鉴定出32种蛋白质,包括上调蛋白有20种,下调蛋白有12种.从功能上可分为载体蛋白(3种)、信号转导蛋白(4种)、增殖凋亡相关蛋白(2种)、细胞骨架蛋白(6种)、细胞外基质蛋白(8种)、免疫因子(3种)、肿瘤相关蛋白(2种)和未知功能蛋白(4种).结论蛋白质组学能较好地显示瘢痕疙瘩与正常皮肤组织间的蛋白质表达差异.对这些差异蛋白质进一步深入研究,将有助于揭示瘢痕疙瘩的发病机制,也为发现新的治疗靶点提供线索和依据.
목적 통과비교반흔흘탑여정상피부조직적단백질조표체차이,사선출여반흔흘탑산생상관적단백질.방법 2010년1월지6월운용단백질조학기술,대8례반흔흘탑조직화3례정상피부조직진행차이쌍향응효전영(2D-DIGE),선택차이단백질반점,진행기질보조격광해리비행시간(MALDI-TOF/TOF)질보분석화생물학신식분석.결과 성공건립반흔흘탑화정상피부조직적쌍향응효전영도보,반흔흘탑화정상피부조직응효전영도보중평균단백질반점수분별위2978화3053,기중표체차이초과4배적반점공유40개,질보분석화수거고검색공감정출32충단백질,포괄상조단백유20충,하조단백유12충.종공능상가분위재체단백(3충)、신호전도단백(4충)、증식조망상관단백(2충)、세포골가단백(6충)、세포외기질단백(8충)、면역인자(3충)、종류상관단백(2충)화미지공능단백(4충).결론 단백질조학능교호지현시반흔흘탑여정상피부조직간적단백질표체차이.대저사차이단백질진일보심입연구,장유조우게시반흔흘탑적발병궤제,야위발현신적치료파점제공선색화의거.
Objective To investigate and search correlative proteins of keloid by comparing the results of differential proteomic analysis between keloid and normal skin. Methods From January 2010 to June 2010 two-dimensional gel electrophoresis was used to define patterns of protein expression in keloid skin from 8 patients and matched normal skin from 3 patients. Differential expression protein spots were showed and analyzed by matrix-assisted laser desorption ionization-time of flying/time of flying(MALDI-TOF/TOF) mass spectrometry. Results This study succeeded to provide a two-dimensional protein profiling comparison between normal skin and keloid. Gel-analysis software identified an average of 2978 spots in keloid while 30S3 spots in normal skin and statistical filtering yielded 40 spots of a 4-fold change, 32 of which were identified by using mass spectrometry, 20 were up-regulated and 12 were down-regulated. Functional analysis revealed that these proteins could be fractionated to carrier proteins (3 proteins), signal transduction proteins (4 proteins) , proliferation and apoptosis related proteins (2 proteins) , cytoskeleton proteins(6 proteins) , extracellular matrix proteins(8 proteins) , immunity related proteins (3 proteins) , tumor related proteins (2 proteins) , and function unknown protein (4 proteins). Conclusions Proteomic analysis can identify the proteins with variance of keloid versus normal skin. The further research to these differential proteins may help reveal the pathogenesis of keloid and provide new treatments for keloid.