背景:内皮素、一氧化氮作为一对维持血管张力,血流动力的平衡因子,在不同的疾病过程中,均有不同的反应,阐明高脂血症对内皮细胞的影响及病理生理机制和寻找保护血管内皮的方法及药物已成为研究动脉粥样硬化的重点.目的:观察隔药饼灸对高脂血症兔血浆内皮素和一氧化氮含量的影响,分析隔药饼灸对高脂血症兔内皮素-一氧化氮的调节作用.设计:随机对照动物实验.单位:湖南中医药大学针推学院.材料:普通级健康新西兰白兔60只,体质量1.5～2.5kg,雌雄不拘.药饼由丹参、山楂、郁金、大黄、泽泻等按一定剂量比例碾为粉末,醋调为糊,制成直径5～8 mm、厚2～3 mm的药饼;艾炷:苏州东方艾绒厂,"神灸300灸"艾炷(型号:东方一号,批号:20021212).方法:实验于2003-11/2004-10完成于湖南中医药大学实验动物中心.实验新西兰白兔随机分为4组,每组15只,即空白对照组、高脂血症模型组、直接灸组、隔药灸组.除空白组外以胆固醇高脂饮食喂养法,建立兔高脂血症模型.选两组穴位轮灸:Ⅰ组:巨阙、天枢(双)、丰隆(双)共五穴;Ⅱ组:心俞(双)、肝俞(双)、脾俞(双)共六穴.于实验开始第1天,选1组穴位,直接灸组兔将艾炷直接粘于穴位上点燃施灸,隔药灸组兔将去除底座的艾炷粘于置于穴位上的药饼上点燃施灸.每穴每次连灸4壮,每日1次,第二天换另一组穴位,两组交替,连灸40 d.其余两组不予任何处理.于实验第41天,对各组动物进行血浆内皮素和一氧化氮含量的测定.采用放射免疫法测定治疗后内皮素的含量,硝酸还原酶法测定一氧化氮含量.主要观察指标:各组实验动物治疗40d后血浆内皮素和一氧化氮的含量. 结果:实验过程中有5只兔因腹泻等原因死亡,其中空白对照组2只,模型组、直接灸组、隔药灸组各1只.最终进入结果分析55只.①隔药灸组血浆内皮素的含量明显低于模型组[(431.57±63.68),(500.14±75.41)ng/L,P＜0.05],但隔药灸组与直接灸组内皮素含量差异无显著性意义[(431.57±63.68),(429.08±77.07)ng/L,P＞0.05].②4组血浆一氧化氮的含量虽然有模型组＜空白组＜隔药饼灸组＜直接灸组的趋势,但4组之间两两比较经统计学处理差异无显著性意义[(27.17±16.55),(29.39±13.24),(30.24±20.25),(30.47±19.62)μmol/L,P＞0.05].结论:隔药饼灸和直接灸均对高脂血症兔血浆内皮素具有明显调节作用,且二者作用相近.对一氧化氮的调节作用不明显.
揹景:內皮素、一氧化氮作為一對維持血管張力,血流動力的平衡因子,在不同的疾病過程中,均有不同的反應,闡明高脂血癥對內皮細胞的影響及病理生理機製和尋找保護血管內皮的方法及藥物已成為研究動脈粥樣硬化的重點.目的:觀察隔藥餅灸對高脂血癥兔血漿內皮素和一氧化氮含量的影響,分析隔藥餅灸對高脂血癥兔內皮素-一氧化氮的調節作用.設計:隨機對照動物實驗.單位:湖南中醫藥大學針推學院.材料:普通級健康新西蘭白兔60隻,體質量1.5～2.5kg,雌雄不拘.藥餅由丹參、山楂、鬱金、大黃、澤瀉等按一定劑量比例碾為粉末,醋調為糊,製成直徑5～8 mm、厚2～3 mm的藥餅;艾炷:囌州東方艾絨廠,"神灸300灸"艾炷(型號:東方一號,批號:20021212).方法:實驗于2003-11/2004-10完成于湖南中醫藥大學實驗動物中心.實驗新西蘭白兔隨機分為4組,每組15隻,即空白對照組、高脂血癥模型組、直接灸組、隔藥灸組.除空白組外以膽固醇高脂飲食餵養法,建立兔高脂血癥模型.選兩組穴位輪灸:Ⅰ組:巨闕、天樞(雙)、豐隆(雙)共五穴;Ⅱ組:心俞(雙)、肝俞(雙)、脾俞(雙)共六穴.于實驗開始第1天,選1組穴位,直接灸組兔將艾炷直接粘于穴位上點燃施灸,隔藥灸組兔將去除底座的艾炷粘于置于穴位上的藥餅上點燃施灸.每穴每次連灸4壯,每日1次,第二天換另一組穴位,兩組交替,連灸40 d.其餘兩組不予任何處理.于實驗第41天,對各組動物進行血漿內皮素和一氧化氮含量的測定.採用放射免疫法測定治療後內皮素的含量,硝痠還原酶法測定一氧化氮含量.主要觀察指標:各組實驗動物治療40d後血漿內皮素和一氧化氮的含量. 結果:實驗過程中有5隻兔因腹瀉等原因死亡,其中空白對照組2隻,模型組、直接灸組、隔藥灸組各1隻.最終進入結果分析55隻.①隔藥灸組血漿內皮素的含量明顯低于模型組[(431.57±63.68),(500.14±75.41)ng/L,P＜0.05],但隔藥灸組與直接灸組內皮素含量差異無顯著性意義[(431.57±63.68),(429.08±77.07)ng/L,P＞0.05].②4組血漿一氧化氮的含量雖然有模型組＜空白組＜隔藥餅灸組＜直接灸組的趨勢,但4組之間兩兩比較經統計學處理差異無顯著性意義[(27.17±16.55),(29.39±13.24),(30.24±20.25),(30.47±19.62)μmol/L,P＞0.05].結論:隔藥餅灸和直接灸均對高脂血癥兔血漿內皮素具有明顯調節作用,且二者作用相近.對一氧化氮的調節作用不明顯.
배경:내피소、일양화담작위일대유지혈관장력,혈류동력적평형인자,재불동적질병과정중,균유불동적반응,천명고지혈증대내피세포적영향급병리생리궤제화심조보호혈관내피적방법급약물이성위연구동맥죽양경화적중점.목적:관찰격약병구대고지혈증토혈장내피소화일양화담함량적영향,분석격약병구대고지혈증토내피소-일양화담적조절작용.설계:수궤대조동물실험.단위:호남중의약대학침추학원.재료:보통급건강신서란백토60지,체질량1.5～2.5kg,자웅불구.약병유단삼、산사、욱금、대황、택사등안일정제량비례년위분말,작조위호,제성직경5～8 mm、후2～3 mm적약병;애주:소주동방애융엄,"신구300구"애주(형호:동방일호,비호:20021212).방법:실험우2003-11/2004-10완성우호남중의약대학실험동물중심.실험신서란백토수궤분위4조,매조15지,즉공백대조조、고지혈증모형조、직접구조、격약구조.제공백조외이담고순고지음식위양법,건립토고지혈증모형.선량조혈위륜구:Ⅰ조:거궐、천추(쌍)、봉륭(쌍)공오혈;Ⅱ조:심유(쌍)、간유(쌍)、비유(쌍)공륙혈.우실험개시제1천,선1조혈위,직접구조토장애주직접점우혈위상점연시구,격약구조토장거제저좌적애주점우치우혈위상적약병상점연시구.매혈매차련구4장,매일1차,제이천환령일조혈위,량조교체,련구40 d.기여량조불여임하처리.우실험제41천,대각조동물진행혈장내피소화일양화담함량적측정.채용방사면역법측정치료후내피소적함량,초산환원매법측정일양화담함량.주요관찰지표:각조실험동물치료40d후혈장내피소화일양화담적함량. 결과:실험과정중유5지토인복사등원인사망,기중공백대조조2지,모형조、직접구조、격약구조각1지.최종진입결과분석55지.①격약구조혈장내피소적함량명현저우모형조[(431.57±63.68),(500.14±75.41)ng/L,P＜0.05],단격약구조여직접구조내피소함량차이무현저성의의[(431.57±63.68),(429.08±77.07)ng/L,P＞0.05].②4조혈장일양화담적함량수연유모형조＜공백조＜격약병구조＜직접구조적추세,단4조지간량량비교경통계학처리차이무현저성의의[(27.17±16.55),(29.39±13.24),(30.24±20.25),(30.47±19.62)μmol/L,P＞0.05].결론:격약병구화직접구균대고지혈증토혈장내피소구유명현조절작용,차이자작용상근.대일양화담적조절작용불명현.
BACKGROUND: Endothelin and nitrogen monoxide (NO) which are regarded as a pair of factors to maintain equilibrium between vascular stress and hemodynamics have different responses during various diseases. It is the key point for the treatment of atherosclerosis to find out the effect and pathophysiological mechanism of hyperlipemia on endothelial cells, and seek the methods and drugs for protecting vascular endothelin.OBJECTIVE: To observe the effect of herbal cake-separated moxibustion on contents of plasma endothelin and NO and analyze its regulative mechanism.DESIGN: Randomized controlled animal study.SETTING: College of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine.MATERIALS: A total of 60 healthy New Zealand white rabbits weighing 1.5-2.5 kg of both genders were selected in this study. Herbal cake was made of danshen, shanzha, yujin, dahuang and zexie, which were crushed into powder according to a certain ratio. Then, vinegar was added to make paste which was 5-8 mm in diameter and 2-3 mm in depth. Moxa cone:(Shenjiu 300) was provided by Suzhou Dongfang Airong Factory (type:Dongfang I; batch number: 20021212).METHODS: The experiment was carried out in the Experimental Animal Center of Hunan University of Traditional Chinese Medicine between November 2003 and October 2004. All rabbits were randomly divided into 4 groups: blank control group, hyperlipemia model group, direct moxibustion group and herbal cake-separated moxibustion group with 15 in each group. Except blank control group, rabbits in other three groups were fed with high fat forage to establish hyperlipemia models. Two groups of acupoints were acupunctured alternatively: Group Ⅰ: Juque (CV14), Tianshu (ST25) at bilateral sides, and Fenglong (ST 40) at bilateral sides; Group Ⅱ:Xinshu(BL15),Ganshu(BL18) and Pishu(BL20), at bilateral sides respectively.On the first day, moxa cone was directly adherent to acupoints of rabbits in direct moxibustion group and lighten for acupuncture, but in herbal cakeseparated moxibustion group, moxa cone which was gotten rid of the carriage was adherent to herbal cake which was put on acupoints and lighted for acupuncture. Each acupoint was acupunctured for 4 successive dosages for once a day. On the next day, the other group of acupoints was acupunctured.The course was 40 days. Rabbits in other two groups were not treated with any ways. On the 41st day, contents of plasma endothelin and NO were measured in each group with radio-immunity method and nitrate-reductase reduction method, respectively.MAIN OUTCOME MEASURES: Contents of plasma endothelin and NO of rabbits after 40-day treatment.RESULTS: Five rabbits died because of diarrhoea or other reasons, including 2 in blank control group, 1 in model group, 1 in direct moxibustion group and 1 in herbal cake-separated moxibustion group. Therefore, 55 rabbits were involved in the final analysis. ① Content of plasma endothelin was lower in herbal cake-separated moxibustion group than that in model group [(431.57±63.68), (500.14±75.41) ng/L, P ＜ 0.05], but there was no significant difference between herbal cake-separated moxibustion group and direct moxibustion group [(431.57±63.68), (429.08±77.07) ng/L, P ＞ 0.05]. ② On NO content, there was an increasing tendency of model group ＜ blank group ＜ herbal cake-separated moxibustion group ＜ direct moxibustion, but there was no significant difference between any two groups [(27.17±16.55),(29.39±13.24), (30.24±20.25), (30.47±19.62) μmol/L, P ＞ 0.05].CONCLUSION: Effects of both two methods of moxibustion in the rabbits with hyperlipemia on content of endothelin are significant and similar, but there are no significant effects on NO content.
