CAJ | 학술논문

目的:观察高强度聚焦超声(high intensity focused ultrasound,HIFU ) 消融兔VX2种植性乳腺肿瘤后,"即刻"这一时间点的肿瘤组织病理学变化.方法:36只新西兰兔接受VX2肿瘤种植后2周,随机分为治疗组24只和对照组12只.治疗组在B型超声监控下接受HIFU消融治疗后即刻取材,HE染色后在光学显微镜下观察病理组织学变化,然后进行电子显微镜观察、琥珀酸脱氢酶(succinate dehydrogenase,SDH)活性检测和肿瘤抗原性免疫组织化学检测.结果:组织学观察发现,HIFU消融后的靶区内肿瘤细胞形态和细胞核型无明显改变,细胞质出现空泡样变;电子显微镜观察显示,肿瘤细胞呈凝固性坏死;SDH活性检测显示肿瘤细胞失活;增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)在对照组的肿瘤细胞内呈阳性表达,而在治疗组的肿瘤细胞中不表达. 结论:VX2种植性乳腺肿瘤经HIFU消融后, 组织学观察到看似正常的肿瘤细胞经电子显微镜、酶学和免疫组织化学检测证实已经死亡.
목적:관찰고강도취초초성(high intensity focused ultrasound,HIFU ) 소융토VX2충식성유선종류후,"즉각"저일시간점적종류조직병이학변화.방법:36지신서란토접수VX2종류충식후2주,수궤분위치료조24지화대조조12지.치료조재B형초성감공하접수HIFU소융치료후즉각취재,HE염색후재광학현미경하관찰병리조직학변화,연후진행전자현미경관찰、호박산탈경매(succinate dehydrogenase,SDH)활성검측화종류항원성면역조직화학검측.결과:조직학관찰발현,HIFU소융후적파구내종류세포형태화세포핵형무명현개변,세포질출현공포양변;전자현미경관찰현시,종류세포정응고성배사;SDH활성검측현시종류세포실활;증식세포핵항원(proliferating cell nuclear antigen,PCNA)재대조조적종류세포내정양성표체,이재치료조적종류세포중불표체. 결론:VX2충식성유선종류경HIFU소융후, 조직학관찰도간사정상적종류세포경전자현미경、매학화면역조직화학검측증실이경사망.
Objective:To observe the pathological changes of VX2 implanted breast cancer ablated by high intensity focused ultrasound (HIFU) in rabbits. Methods:Thirty-six rabbits implanted VX2 breast carcinoma were randomly divided into treatment group (n=24) and control group (n=12) two weeks after implantation. HIFU ablation was performed in the treatment group guided by ultrasound B. The pathological changes were observed under light microscope after hematoxylin-eosin staining and then observed under electron microscope. The activity of succinate dehydrogenase (SDH) was measured. Proliferating cell nuclear antigen (PCNA) was determined by immunohistochemistry. Results:Histopathologic observation found that the morphological features and nucleus karyotypes of tumor cells had no significant changes, but vacuole-like structure appeared in cytoplasm. Typical coagulation necrosis of tumor cells were observed under electron microscope. SDH activity detection showed that tumor cells were inactivated. The expressions of PCNA was positive in control group and negative in treatment group. Conclusion:Electron microscopy, enzyme property test, and immunohistochemistry verified that VX2 implanted breast cancer cells were dead after HIFU ablation in rabbits.