植物病理学报
植物病理學報
식물병이학보
ACTA PHYTOPATHOLOGICA SINICA
2010年
1期
40-50
,共11页
香蕉束顶病毒%基因克隆%序列分析
香蕉束頂病毒%基因剋隆%序列分析
향초속정병독%기인극륭%서렬분석
banana bunchy top virus%gene cloning%sequencing
以海口香蕉柬顶病株的幼嫩假茎和叶片总DNA为模板,通过反向PCR法克隆了香蕉束顶病毒(Banana bunchy topvirus,BBTV)海口分离物(命名为BBTV-HaiKou)的6个DNA组分的全长序列.结果表明,DNA1～6序列全长分别为1 106、 040、1 058、1 040、1 013和1 082 nt.各组分非编码区各包含一个茎环共同区和一个主要共同区,同源性分别为91.55%和88.45%.各组分编码区均编码一个开放可读框(ORF),其中DNAl ORF内部还有一个小的ORF.序列分析表明,BBTV-HaiKou分离物与其它分离物间的DNA1最为保守,DNA2变异最大.DNA1组分核苷酸序列与亚洲组、南太平洋组各分离物及ABTV(Abac(a)bunchy top virus)分离物同源性分别为93.1%～99.1%.89.6%～0.7%和76.2%～77.4%.相应的编码蛋白氨基酸序列同源性在BBTV和ABTV中分别为93.4%～100%和85.7%.根据Karan等的分类方法.确定BBTV-HaiKou分离物属于亚洲组成员.
以海口香蕉柬頂病株的幼嫩假莖和葉片總DNA為模闆,通過反嚮PCR法剋隆瞭香蕉束頂病毒(Banana bunchy topvirus,BBTV)海口分離物(命名為BBTV-HaiKou)的6箇DNA組分的全長序列.結果錶明,DNA1～6序列全長分彆為1 106、 040、1 058、1 040、1 013和1 082 nt.各組分非編碼區各包含一箇莖環共同區和一箇主要共同區,同源性分彆為91.55%和88.45%.各組分編碼區均編碼一箇開放可讀框(ORF),其中DNAl ORF內部還有一箇小的ORF.序列分析錶明,BBTV-HaiKou分離物與其它分離物間的DNA1最為保守,DNA2變異最大.DNA1組分覈苷痠序列與亞洲組、南太平洋組各分離物及ABTV(Abac(a)bunchy top virus)分離物同源性分彆為93.1%～99.1%.89.6%～0.7%和76.2%～77.4%.相應的編碼蛋白氨基痠序列同源性在BBTV和ABTV中分彆為93.4%～100%和85.7%.根據Karan等的分類方法.確定BBTV-HaiKou分離物屬于亞洲組成員.
이해구향초간정병주적유눈가경화협편총DNA위모판,통과반향PCR법극륭료향초속정병독(Banana bunchy topvirus,BBTV)해구분리물(명명위BBTV-HaiKou)적6개DNA조분적전장서렬.결과표명,DNA1～6서렬전장분별위1 106、 040、1 058、1 040、1 013화1 082 nt.각조분비편마구각포함일개경배공동구화일개주요공동구,동원성분별위91.55%화88.45%.각조분편마구균편마일개개방가독광(ORF),기중DNAl ORF내부환유일개소적ORF.서렬분석표명,BBTV-HaiKou분리물여기타분리물간적DNA1최위보수,DNA2변이최대.DNA1조분핵감산서렬여아주조、남태평양조각분리물급ABTV(Abac(a)bunchy top virus)분리물동원성분별위93.1%～99.1%.89.6%～0.7%화76.2%～77.4%.상응적편마단백안기산서렬동원성재BBTV화ABTV중분별위93.4%～100%화85.7%.근거Karan등적분류방법.학정BBTV-HaiKou분리물속우아주조성원.
The genome of the isolate of Banana bunchy top virus(BBTV)from Haikou was cloned and sequenced by the polymerase chain reaction(PCR)With total DNA from banana pseudostem and leaf showing typical BBTV symptom as template.The isolate originated from banana(Musa sp.)was designated BBTV Haikou.The sequence analysis indicated that six full-length segments were 1106,1040,1058,1040,1013 and 1 082 nt,respectively.All the segments contained an intergenic region and an open reading frame(ORF)except the segment 1,which had a small ORF in the main ORF.In the intergenic region(IR),there were a stem-loop common region(CR-SL)and a major common region(CR-M),which shared 91.55%and 88.45%identity,respectively.Comparison of the viral sequence from different country showed that DNA1 sequence was more conservative than other components,but DNA2 sequence mutation rate was higher than others,sharing homologies of 93.08%and 75.67%,respectively.Nucleotide sequence analysis between DNA1 of the isolate and that of the Asian group,Pacific group of BBTV and the two isolates of ABTV(Abac(a)bunchy top virus)showed that DNA1 of BBTV-Haikou shared 93.1%-99.1%.89.6%-90.7%and 76.2% -77.4% nucleotide sequence identity.also 93.4%～100%and 85.7%deduced amino acids identity in BBTV and ABTV,respectively.According to the Karan's method of classification,the BBTV-Haikou isolate was belonged to the Asian group.
