CAJ | 학술논문

目的　测定自制纳米级脂质体超声微泡造影剂(nano-liposomal bubble,NB)的基本特性.方法　采用逆向蒸发法制备脂质体,用声震法制备NB后观察其形态,检测其平均粒径、表面电位及浓度等物理特性；在脱气水中分别注入生理盐水、NB及SonoVue (200μl),观察显影效果；分别经兔耳缘静脉团注生理盐水、NB及SonoVue (2.0 ml/kg),动态观察兔心脏及肝的增强情况.结果　镜下观察,NB粒径范围133.1～199.5 nm,平均粒径为(171.60±30.82)nm,平均电位为- (1.92±0.65)mV,分布均匀,浓度为(3.8～5.6)×108/ml.常温下造影剂放置1周、1月后观察,基本特性无明显改变.体外显影结果显示:NB与SonoVue一样具有显著的显影效果.体内造影后观察:NB与SonoVue均能明显增强兔心脏及肝的二维灰阶显像.结论　NB性质稳定,形态好,粒径均一,具有良好的显影效果.由于其粒径小且基于脂质体基础上易被修饰,因此在超声分子影像及基因/药物传递方面具有广阔的应用前景.
목적　측정자제납미급지질체초성미포조영제(nano-liposomal bubble,NB)적기본특성.방법　채용역향증발법제비지질체,용성진법제비NB후관찰기형태,검측기평균립경、표면전위급농도등물리특성；재탈기수중분별주입생리염수、NB급SonoVue (200μl),관찰현영효과；분별경토이연정맥단주생리염수、NB급SonoVue (2.0 ml/kg),동태관찰토심장급간적증강정황.결과　경하관찰,NB립경범위133.1～199.5 nm,평균립경위(171.60±30.82)nm,평균전위위- (1.92±0.65)mV,분포균균,농도위(3.8～5.6)×108/ml.상온하조영제방치1주、1월후관찰,기본특성무명현개변.체외현영결과현시:NB여SonoVue일양구유현저적현영효과.체내조영후관찰:NB여SonoVue균능명현증강토심장급간적이유회계현상.결론　NB성질은정,형태호,립경균일,구유량호적현영효과.유우기립경소차기우지질체기출상역피수식,인차재초성분자영상급기인/약물전체방면구유엄활적응용전경.
Objective To made a kind of nano-liposomal bubbles(NB) and observe its base physical properties and ultrasound contrast enhanced effect,and compared with SonoVue in vitro and in vivo.Methods The liposome was made with the method of reverse phase evaporation,and then the liposome was sonieated to prepare NB.After the physical properties of NB such as its morphous,average particle size,surface potential and concentration were observed and calculated,200 μl of saline and two contrast agents (NB and SonoVue) were injected into degassed PBS (10 ml) respectively to observe their enhancing effects.For in vivo study,a healthy rabbit heart and liver were imaged before and after intravenous injection of 2.0 ml/kg of saline and two contrast agents in succession to compare their dynamic enhancing effects in the grey scale imaging subjectively.Results The self-made NB distributed uniformly and its size ranged from 133.1 ～199.5 nm with a mean diameter of (171.60 ± 30.82) nm.Its surface potential and concentration were -(1.92± 0.65)mV and (3.8 ～ 5.6) × 108/ml individually.These basic characteristics were not observed changing dramatically after placement one week to one month under room temperature.The NB and SonoVue all displayed significantly enhancing effect comparing with saline no matter in vitro or in vivo.Conclusions The self-made NB are stable and distribute uniformly,which display the same contrast enhancing effect as SonoVue in vitro and in vivo.The self made NB would have a more potential use in ultrasound molecular imaging and gone or drug delivery.