目的 通过微卫星遗传标记对双相情感障碍患者及正常对照者的4号染色体进行扫描,查找与双相情感障碍关联的遗传位点,进而定位易感基因.方法 在4号染色体上间隔10cM(厘摩)遗传距离选择了22个微卫星遗传标记,对104例发病年龄≤20岁的双相情感障碍患者与1000例正常对照者组成的DNA混合样本分别进行了基因扫描及分型.采用CLUMP软件进行统计学分析,逐一比较患者组与对照组每个等位基因频率的差异.结果 在4号染色体D4S1592和D4S402两个位点发现患者组与对照组的等位基因频率差异有统计学意义(其中在D451592位点共得到7个等位基因片段,患者组的等位基因频率分别为0.062,0.097,0.151,0.266,0.196,0.189,0.039,对照组的等位基因频率分别为0.063,0.182,0.172,0.283,0.166,0.124,0.010,x2=15.968,P=0.006;在D4S402位点共得到13个等位基因片段,患者组的等位基因频率分别为0.015,0.023,0.033,0.046,0.096,0.162,0.149,0.165,0.137,0.069,0.036,0.041,0.030,对照组的等位基因频率分别为0.023,0.032,0.051,0.093,0.151,0.179,0.158,0.130,0.078,0.048,0.029,0.017,0.010,x2=31.553,P=0.002).结论 山东省双相情感障碍患者与4号染色体上D4S1592和D4S402相关联,易感基因可能位于其附近.
目的 通過微衛星遺傳標記對雙相情感障礙患者及正常對照者的4號染色體進行掃描,查找與雙相情感障礙關聯的遺傳位點,進而定位易感基因.方法 在4號染色體上間隔10cM(釐摩)遺傳距離選擇瞭22箇微衛星遺傳標記,對104例髮病年齡≤20歲的雙相情感障礙患者與1000例正常對照者組成的DNA混閤樣本分彆進行瞭基因掃描及分型.採用CLUMP軟件進行統計學分析,逐一比較患者組與對照組每箇等位基因頻率的差異.結果 在4號染色體D4S1592和D4S402兩箇位點髮現患者組與對照組的等位基因頻率差異有統計學意義(其中在D451592位點共得到7箇等位基因片段,患者組的等位基因頻率分彆為0.062,0.097,0.151,0.266,0.196,0.189,0.039,對照組的等位基因頻率分彆為0.063,0.182,0.172,0.283,0.166,0.124,0.010,x2=15.968,P=0.006;在D4S402位點共得到13箇等位基因片段,患者組的等位基因頻率分彆為0.015,0.023,0.033,0.046,0.096,0.162,0.149,0.165,0.137,0.069,0.036,0.041,0.030,對照組的等位基因頻率分彆為0.023,0.032,0.051,0.093,0.151,0.179,0.158,0.130,0.078,0.048,0.029,0.017,0.010,x2=31.553,P=0.002).結論 山東省雙相情感障礙患者與4號染色體上D4S1592和D4S402相關聯,易感基因可能位于其附近.
목적 통과미위성유전표기대쌍상정감장애환자급정상대조자적4호염색체진행소묘,사조여쌍상정감장애관련적유전위점,진이정위역감기인.방법 재4호염색체상간격10cM(전마)유전거리선택료22개미위성유전표기,대104례발병년령≤20세적쌍상정감장애환자여1000례정상대조자조성적DNA혼합양본분별진행료기인소묘급분형.채용CLUMP연건진행통계학분석,축일비교환자조여대조조매개등위기인빈솔적차이.결과 재4호염색체D4S1592화D4S402량개위점발현환자조여대조조적등위기인빈솔차이유통계학의의(기중재D451592위점공득도7개등위기인편단,환자조적등위기인빈솔분별위0.062,0.097,0.151,0.266,0.196,0.189,0.039,대조조적등위기인빈솔분별위0.063,0.182,0.172,0.283,0.166,0.124,0.010,x2=15.968,P=0.006;재D4S402위점공득도13개등위기인편단,환자조적등위기인빈솔분별위0.015,0.023,0.033,0.046,0.096,0.162,0.149,0.165,0.137,0.069,0.036,0.041,0.030,대조조적등위기인빈솔분별위0.023,0.032,0.051,0.093,0.151,0.179,0.158,0.130,0.078,0.048,0.029,0.017,0.010,x2=31.553,P=0.002).결론 산동성쌍상정감장애환자여4호염색체상D4S1592화D4S402상관련,역감기인가능위우기부근.
Objective To find out association mapping of loci related to bipolar disorder on chromosome 4 with microsatellite markers in DNA pooling samples from bipolar disorder cases and normal controls in Shandong province. Methods A total of 22 microsatellite markers on chromosome 4 spaced at approximately 10 cM were selected and two separated DNA pooling samples consisting of 104 bipolar disorder cases and 1000 normal controls were genotyped respectively. Statistic analysis was performed by Chi-square method with CLUMP software to compare the difference in the ratio of each allele in these loci between the two pooling samples. Result Significant statistic differences were found at D4S1592 and D4S402 on chromosome 4 between cases and controls(P＜0.01 ).( D4S1592:x2 = 15.968, P=0.006; D4S402:x2 =31.553, P=0.002). Conclusion The loci of D4S1592 and D4S402 on chromosome 4 are found to be associated with bipolar disorder patients in Shandong province, further screening of the susceptibility genes around these loci is needed.
