CAJ | 학술논문

目的制备高效、特异的抗河流弧菌单克隆抗体(monoclonal antibody,mAb),为海洋致病细菌的快速免疫诊断提供技术支持.方法选用雌性BALB/c小鼠(8周龄)制备成为灭活河流弧菌免疫小鼠,利用杂交瘤细胞融合技术,制备出抗河流弧菌杂交瘤细胞株,用Giemsa染色对其进行染色体鉴定,并用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)方法筛选其与河流弧菌及其他重要海洋细菌的交叉反应特异性.结果共获得4株抗河流弧菌mAb,均符合杂交瘤细胞染色体特点,具有良好的特异性和免疫反应性,分别命名为01H10,02F12,05F3和03G10.结论本研究制备、筛选出抗河流弧菌的mAb,有助于建立抗河流弧菌快速免疫检测试剂盒.
목적 제비고효、특이적항하류호균단극륭항체(monoclonal antibody,mAb),위해양치병세균적쾌속면역진단제공기술지지.방법 선용자성BALB/c소서(8주령)제비성위멸활하류호균면역소서,이용잡교류세포융합기술,제비출항하류호균잡교류세포주,용Giemsa염색대기진행염색체감정,병용매련면역흡부시험(enzyme linked immunosorbent assay,ELISA)방법사선기여하류호균급기타중요해양세균적교차반응특이성.결과 공획득4주항하류호균mAb,균부합잡교류세포염색체특점,구유량호적특이성화면역반응성,분별명명위01H10,02F12,05F3화03G10.결론 본연구제비、사선출항하류호균적mAb,유조우건립항하류호균쾌속면역검측시제합.
Objective To prepare high-performance and specific monoclonal antibody of vibrio fluvialis,and to provide a technical platform for the development of a rapid ELISA kit.Methods BALB/c mice (8 weeks) were immunized with inactivated vibrio fluvialis,and hybridoma cell strains of anti-vibrio fluvialis were prepared with cell-fusion technology.Chromatosomes of hybridoma cells were identified with Giemsa staining,and the potency and cross-reactivity of anti-vibrio fluvialis as well as other important marine bacteria were screened and identified with ELISA.Results Four strains of hybridoma (named as 01H10,02F12,05F3 and 03G10 ) were obtained,all having the features of hybridoma cell chromatosomes,and with good specificity and immunorcactivity.The hybridoma also had favourable specificity and immunoreactivity.Conclusions Specific antibodies of anti-vibriofluvialis prepared and screened in the study would be useful for the development of the rapid detection kit of vibrio fluvialis.