CAJ | 학술논문

目的探讨重组人白细胞介素-11(rhIL-11)和姜黄素对中子照射致小鼠肠道损伤的治疗作用.方法选取二级雄性BALB/c小鼠140只,随机分为健康对照组(20只)、单纯照射组(60只)、IL-11治疗组(30只)和姜黄素治疗组(30只).单纯照射组小鼠采用3 Gy中子全身均匀照射,两个治疗组于照射后即日分别腹腔注射500μg·kg-1·d-1的rhIL-11或200 mg·kg-1·d-1的姜黄素,每天1次,连续给药5 d.观察各组动物的死亡率.照射后6 h、1、3和5 d分别处死健康对照组和单纯照射组动物,3和5 d分别处死两个治疗组动物.取空肠标本,用HE深色、核仁组成区嗜银蛋白染色、Feulgen氏染色和图像死亡分析等,检测空肠病理形态变化、空肠上皮细胞核内嗜银蛋白含量和DNA含量变化.结果照后5 d,单纯照射组小鼠全部死亡,rhIL-11治疗组存活剩余2只,姜黄素治疗组存活剩余3只.3 Gy中子照射后6 h～3 d内,空肠黏膜大面积坏死脱落,3 d偶见隐窝细胞再生,5 d见较多新生隐窝;IL-11治疗组在照后3 d隐窝再生较明显,5 d见大量新生绒毛;姜黄素治疗组在照后3 d隐窝有部分再生,5 d可见新生绒毛,排列结构较IL-11治疗组紊乱,绒毛高度较IL-11治疗组略低.照后5 d,两个治疗组小鼠空肠上皮细胞AgNOR含量和DNA含量均高于单纯照射组(F=0.015～0.035,P＜0.05).结论 3 Gy中子照射引起小鼠空肠明显损伤,rhIL-11和姜黄素治疗可减轻损伤并促进肠道上皮再生修复,对中子辐射肠上皮损伤具有保护作用.
목적 탐토중조인백세포개소-11(rhIL-11)화강황소대중자조사치소서장도손상적치료작용.방법 선취이급웅성BALB/c소서140지,수궤분위건강대조조(20지)、단순조사조(60지)、IL-11치료조(30지)화강황소치료조(30지).단순조사조소서채용3 Gy중자전신균균조사,량개치료조우조사후즉일분별복강주사500μg·kg-1·d-1적rhIL-11혹200 mg·kg-1·d-1적강황소,매천1차,련속급약5 d.관찰각조동물적사망솔.조사후6 h、1、3화5 d분별처사건강대조조화단순조사조동물,3화5 d분별처사량개치료조동물.취공장표본,용HE심색、핵인조성구기은단백염색、Feulgen씨염색화도상사망분석등,검측공장병리형태변화、공장상피세포핵내기은단백함량화DNA함량변화.결과 조후5 d,단순조사조소서전부사망,rhIL-11치료조존활잉여2지,강황소치료조존활잉여3지.3 Gy중자조사후6 h～3 d내,공장점막대면적배사탈락,3 d우견은와세포재생,5 d견교다신생은와;IL-11치료조재조후3 d은와재생교명현,5 d견대량신생융모;강황소치료조재조후3 d은와유부분재생,5 d가견신생융모,배렬결구교IL-11치료조문란,융모고도교IL-11치료조략저.조후5 d,량개치료조소서공장상피세포AgNOR함량화DNA함량균고우단순조사조(F=0.015～0.035,P＜0.05).결론 3 Gy중자조사인기소서공장명현손상,rhIL-11화강황소치료가감경손상병촉진장도상피재생수복,대중자복사장상피손상구유보호작용.
Objective To explore the therapeutic effect of recombinant human interleukin(rhIL-11) and curcumin on jejunal damage in mice after neutron irradiation.Methods 140 male BALB/c mice were randomly divided into 4 groups:20 mice in healthy control group,60 mice in mere irradiation group,30 mice in IL-11 treatment group and 30 mice in curcumin treatment group.The mere irradiation group mice were wholly exposed to 3 Gy neutron irradiation.The treatment groups mice were intraperitoneally enterocoelia once a day for 5 d after irradiation.The mortality of the mice were observed.The mice in the control and mere irradiation groups were killed 6 h,1,3,and 6 d post-irradiation,respectively,and the mice of the 2 treatment groups were killed 3 and 6 d post-irradiation,respectively and the samples of jujunum were colleted.HE staining,argyrophilic of nucleaolar organizer regions staining,Feulgen staining,and image analysis were used to observe the pathology and levels of argyrophilic proteins and DNA.Results The mice in the mere irradiation group all died at 5 d post-irradiation,while 2 mice in the IL-11 treatment group and 3 in the curcumin group survived.Large area necrosis and exfoliation were found in the intestinal epithelial mucosa of the mere irradiated group mice since 6 h to 3 d after irradiation.Crypt cell regeneration was seen occasionally found 3 days later and much more 5 days later.Crypt cell regeneration was obviously found in the intestinal epithelial mucosa and lots of new villi were observed 5 d after irradiation in both treatment groups,however,the amounts of crypt cells and new villi of the curcumin treatment group were less than those of the IL-11 treatment group.The contents of AgNOR and DNA in the intestinal epithelial cells 5 days after irradiation of the 2 treatment groups were all significantly higher than those of the mere irradiation group (F = 0.015-0.035,all P ＜ 0.05) but without significant differences between them.Conclusions Jejunal damage in mice could be induced after 3 Gy neutron irradiation.rhIL-11 and curcumin might reduce the damage and promote the regeneration and repair of the intestinal epithelium.