CAJ | 학술논문

目的探讨依那普利拉对烧伤早期内脏损害保护作用的量效关系.方法随机将54只SD大鼠分为烫伤组(B组)和烫伤后小剂量、中剂量和大剂量依那普利拉治疗组(E1、E2、E3组),每组12只大鼠,另取6只大鼠不致伤作为正常对照组.检测烫伤前及伤后6 h和12 h动脉血压、血管紧张素Ⅱ、尿素氮(Bun)、肌酐(Cr)和肌酸激酶(CK)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST),以及主要脏器组织病理变化.结果 B组血管紧张素Ⅱ明显升高,治疗组均明显低于B组(P均＜0.05),E2和E3组明显低于E1组;B组大鼠CK、Bun、Cr、ALT、AST均显著高于正常参考值(P均＜0.05),治疗组较B组不同程度降低,E1组下降明显(P＜0.05),各时相点基本与正常值接近;E2和E3组下降不明显,明显高于E1组;烫伤后各组动脉收缩压和舒张压均有不同程度下降,其中E2、E3组较E1组更明显,E1组较B组明显升高.结论小剂量依那普利拉能够显著减轻严重烧伤后脏器损害的程度,但对动脉收缩压和舒张压无显著影响.
목적 탐토의나보리랍대소상조기내장손해보호작용적량효관계.방법 수궤장54지SD대서분위탕상조(B조)화탕상후소제량、중제량화대제량의나보리랍치료조(E1、E2、E3조),매조12지대서,령취6지대서불치상작위정상대조조.검측탕상전급상후6 h화12 h동맥혈압、혈관긴장소Ⅱ、뇨소담(Bun)、기항(Cr)화기산격매(CK)、병안산전안매(ALT)、천동안산전안매(AST),이급주요장기조직병리변화.결과 B조혈관긴장소Ⅱ명현승고,치료조균명현저우B조(P균＜0.05),E2화E3조명현저우E1조;B조대서CK、Bun、Cr、ALT、AST균현저고우정상삼고치(P균＜0.05),치료조교B조불동정도강저,E1조하강명현(P＜0.05),각시상점기본여정상치접근;E2화E3조하강불명현,명현고우E1조;탕상후각조동맥수축압화서장압균유불동정도하강,기중E2、E3조교E1조경명현,E1조교B조명현승고.결론 소제량의나보리랍능구현저감경엄중소상후장기손해적정도,단대동맥수축압화서장압무현저영향.
Objective To investigate the dose-effect relationship of enalaprilat(ENA)injection on the organ damage following early burn injury in rats.Methods A total of 54 SD rats were subjected to 30% total body surface area Ⅲ scald injury,and were randomly divided into simple scald group(B group,with conventional fluid transfusion after scald),ENA treated group(E1,E2,E3 group,with intraperitoneal enalaprilat injection of 1,2,4 mg/kg after scald respectively).Other 6 rats were taken as normal control.Aortic systolic pressure(AOSP),aortic diastolic blood pressure(AODP),mean arterial pressure(MAP),angiotensin Ⅱ,blood urea nitrogen(Bun),creatinine(Cr),creatinine kinase(CK),alanine aminotransferase(ALT),asparate aminotransferase(AST)of the simple scald group,E1 group,E2 group and E3 group were investigated at 6 h and 12 h post burn.Results Ang Ⅱ,Bun,Cr,CK,ALT,AST levels in ENA treated group after 6 h and 12 hours were significantly lower than those of simple scald group(all P<0.05).AOSP,AODP,MAP in ENA.treated group after 6 and 12 hours were significantly higher than those of simple scald group(all P<0.05).Conclusion Low-dose enalaprilat,injection(1 mg/kg)could alleviate organ damage in post-burned rats,but has little effect on AOSP and AODP.