中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2010年
4期
236-240
,共5页
谭愈昱%林健%黄干%唐炜立%Saikiran K Sedimbi%Carani B Sanjeevi%周智广
譚愈昱%林健%黃榦%唐煒立%Saikiran K Sedimbi%Carani B Sanjeevi%週智廣
담유욱%림건%황간%당위립%Saikiran K Sedimbi%Carani B Sanjeevi%주지엄
糖尿病%1型%人白细胞抗原%杀伤细胞%基因
糖尿病%1型%人白細胞抗原%殺傷細胞%基因
당뇨병%1형%인백세포항원%살상세포%기인
Diabetes mellitus%1 type%HLA antigens%Killer cells%Gene
目的 探讨杀伤细胞免疫球蛋白样受体(KIR)基因和KIR配体人类白细胞抗原-C(HLA-C)基因的多态性与1型糖尿病(T1DM)的关系.方法 采用聚合酶链反应-序列特异性引物技术(PCR-SSP),对湖南汉族180例T1DM患者和199例正常对照者KIR基因、HLA-C基因进行检测和分析.结果 (1)与正常对照相比,T1DM组KIR 2DL1(98.9%比92.O%,OR=7.78,P=0.002)、3DL1(94.3%比86.4%,OR=2.67,P=0.009)和2DS4(83.9%比70.9%,OR=2.14,P=O.003)基因频率增高;(2) T1DM组和正常对照组HLA-C(HLA-C1、HLA-C2)基因频率差异无统计学意义,但T1DM组HLA C1+/C2+(3.9%比9.6%,OR=0.38,P=0.03)基因频率降低;(3)与正常对照组相比,T1DM组KIB/HLA-C基因组合中KIR 2DLI-/HLA-C2-(0.6%比6.0%,OR=0.087,P=0.003)和KIR 2DS1-/HLA-C2-(53.3%比64.8%,OR=0.62,P=0.023)基因频率降低.结论 KIR基因多态性和KIR/HLA-C基因组合与T1DM发病相关.
目的 探討殺傷細胞免疫毬蛋白樣受體(KIR)基因和KIR配體人類白細胞抗原-C(HLA-C)基因的多態性與1型糖尿病(T1DM)的關繫.方法 採用聚閤酶鏈反應-序列特異性引物技術(PCR-SSP),對湖南漢族180例T1DM患者和199例正常對照者KIR基因、HLA-C基因進行檢測和分析.結果 (1)與正常對照相比,T1DM組KIR 2DL1(98.9%比92.O%,OR=7.78,P=0.002)、3DL1(94.3%比86.4%,OR=2.67,P=0.009)和2DS4(83.9%比70.9%,OR=2.14,P=O.003)基因頻率增高;(2) T1DM組和正常對照組HLA-C(HLA-C1、HLA-C2)基因頻率差異無統計學意義,但T1DM組HLA C1+/C2+(3.9%比9.6%,OR=0.38,P=0.03)基因頻率降低;(3)與正常對照組相比,T1DM組KIB/HLA-C基因組閤中KIR 2DLI-/HLA-C2-(0.6%比6.0%,OR=0.087,P=0.003)和KIR 2DS1-/HLA-C2-(53.3%比64.8%,OR=0.62,P=0.023)基因頻率降低.結論 KIR基因多態性和KIR/HLA-C基因組閤與T1DM髮病相關.
목적 탐토살상세포면역구단백양수체(KIR)기인화KIR배체인류백세포항원-C(HLA-C)기인적다태성여1형당뇨병(T1DM)적관계.방법 채용취합매련반응-서렬특이성인물기술(PCR-SSP),대호남한족180례T1DM환자화199례정상대조자KIR기인、HLA-C기인진행검측화분석.결과 (1)여정상대조상비,T1DM조KIR 2DL1(98.9%비92.O%,OR=7.78,P=0.002)、3DL1(94.3%비86.4%,OR=2.67,P=0.009)화2DS4(83.9%비70.9%,OR=2.14,P=O.003)기인빈솔증고;(2) T1DM조화정상대조조HLA-C(HLA-C1、HLA-C2)기인빈솔차이무통계학의의,단T1DM조HLA C1+/C2+(3.9%비9.6%,OR=0.38,P=0.03)기인빈솔강저;(3)여정상대조조상비,T1DM조KIB/HLA-C기인조합중KIR 2DLI-/HLA-C2-(0.6%비6.0%,OR=0.087,P=0.003)화KIR 2DS1-/HLA-C2-(53.3%비64.8%,OR=0.62,P=0.023)기인빈솔강저.결론 KIR기인다태성화KIR/HLA-C기인조합여T1DM발병상관.
Objective To investigate the association of Killer cell immunoglobin-like receptor (KIR) gene and KIRs' ligand (HLA-C) gene polymorphisms with type 1 diabetes(T1DM).Methods Using polymerase chain reaction-sequence specific primer (PCR-SSP) to detect KIR and HLA-C genotype in 180 T1DM patients and 199 healthy controls from Hunan Han population.Results (1) The frequencies of KIR 2DL1 (98.9% vs 92.0%,OR=7.78,P=0.002),3DLl (94.3% vs 86.4%,OR=2.67.P=0.009)and 2DS4(83.9% vs 70.9%,OR=2.14,P=0.003) were significantly higher in T1DM patients than those in the controls.(2) There were no differences in the frequencies of HLA-C1 and HLA-C2 between the patients and the controls,but the frequency of HLAC1+/C2+(3.9% vs 9.6%,OR=0.38,P=0.03) was significantly lower in the T1DM patients.(3) The combination KIR2DL1-/HLA-C2-(0.6% vs 6.0%,OR=0.087,P=0.003) and KIR 2DS1-/HLA-C2-(53.3% vs 64.8%,OR=0.62,P=0.023) was significantly lower in the T1DM patients.Conclusion The KIR gene polymorphism and KIR/HLA-C gene compatibility are associated with T1DM.
