CAJ | 학술논문

采用荧光染色标记细菌(fluorescence labeled bacteria,FLB)技术,研究山羊瘤胃中原虫对细菌的吞噬速率.试验选用3头装有永久瘘管的徐淮山羊作为瘤胃液供体,采用体外培养法研究在精粗比为1:9的情况下,探讨不同丝兰提取物水平(A组0 mg/L、B组35 mg/L、C组70 mg/L)对瘤胃原虫吞噬细菌速率的影响.荧光镜检与回归分析结果表明:原虫吞噬细菌的速率:A组:339.9 cells/(cell·h);B组:314.7 cells/(cell·h);C组:339.9 cells/(cell·h).结果表明荧光标记细菌技术能够应用于瘤胃原虫吞噬细菌速率的研究.[动物营养学报,2009,21(3):417-422][中文全文见<动物营养学报>网站(www.ChinaJAN.com)中文版2009年21卷3期]
채용형광염색표기세균(fluorescence labeled bacteria,FLB)기술,연구산양류위중원충대세균적탄서속솔.시험선용3두장유영구루관적서회산양작위류위액공체,채용체외배양법연구재정조비위1:9적정황하,탐토불동사란제취물수평(A조0 mg/L、B조35 mg/L、C조70 mg/L)대류위원충탄서세균속솔적영향.형광경검여회귀분석결과표명:원충탄서세균적속솔:A조:339.9 cells/(cell·h);B조:314.7 cells/(cell·h);C조:339.9 cells/(cell·h).결과표명형광표기세균기술능구응용우류위원충탄서세균속솔적연구.[동물영양학보,2009,21(3):417-422][중문전문견<동물영양학보>망참(www.ChinaJAN.com)중문판2009년21권3기]
Research on the bacterial predation rate by rumen protozoa was carried out using a technique of fluorescence-labeled bacteria in culture in vitro incubation. Three Xuhuai goats fitted with rumen cannula were used to determine the effects of yucca schidigera extract (YSE) at 0 (group A), 35 (group B) and 70 mg/L (group C) on the determination of bacterial predation rate by rumen protozoa when dietary concentrate to forage ratio was set to 1 : 9 in the culture. The results showed that the predation rates of rumen protozoa were 339. 9 cells/(cell·h) in group A, 314. 7 cells/(cell·h) in group B, 339. 9 cells/(cell·h) in group C, respectively. It was concluded that the fluorescence-labeled bacteria technique would be a potential option for determination of bacterial predation rate by rumen protozoa.