CAJ | 학술논문

目的探讨Alu序列甲基化与乳腺癌转移潜能的关系.方法用亚硫酸氢盐修饰联合限制性内切酶分析法(combined bisulfite restriction analysis,COBRA)、亚硫酸氢盐修饰结合直接测序法(bisulfite sequencing,BSP)检测两株转移能力不同的乳腺癌细胞系MCF7和MDA-MB-435S中Alu甲基化状态,每个样品挑取10个克隆测序.结果 MCF7和MDA-MB-435S中Alu甲基化水平均明显低于报道的正常人体细胞Alu甲基化水平,但MCF7中Alu的甲基化水平明显高于MDA-MB-435S.同时,Alu甲基化位点在基因组中分布不均匀.结论乳腺癌的转移潜能可能与Alu序列的去甲基化以及去甲基化位点的分布相关,值得进一步探讨.
목적 탐토Alu서렬갑기화여유선암전이잠능적관계.방법 용아류산경염수식연합한제성내절매분석법(combined bisulfite restriction analysis,COBRA)、아류산경염수식결합직접측서법(bisulfite sequencing,BSP)검측량주전이능력불동적유선암세포계MCF7화MDA-MB-435S중Alu갑기화상태,매개양품도취10개극륭측서.결과 MCF7화MDA-MB-435S중Alu갑기화수평균명현저우보도적정상인체세포Alu갑기화수평,단MCF7중Alu적갑기화수평명현고우MDA-MB-435S.동시,Alu갑기화위점재기인조중분포불균균.결론 유선암적전이잠능가능여Alu서렬적거갑기화이급거갑기화위점적분포상관,치득진일보탐토.
Objective To determine the relationship between methylation level of Alu elements and breast cancer metastasis.Method COBRA and BSP were employed to detect the methylation level of Alu elements in two breast cancer cell lines (MCF7 and MDA-MB-435S) with significant different metastasis potential. For BSP analysis, ten clones of each sample were analyzed.Result The methylation level of Alu in MDA-MB-435S cells was lower than that in MCF7 cells, although the Alu methylation levels in both MCF7 and MDA-MB-435S cells were lower than those in normal tissue cells reported previously. Moreover, the methylated Alu sequences were non-uniform distributed in the genome.Conclusion Demethylation of Alu elements may be involved in breast cancer metastasis. The correlation between methylation status of Alu and breast cancer metastasis deserves further investigation.