CAJ | 학술논문

为探索水平基因转移在绿球蓝细菌基因组多样性中的作用,5个高光适应性绿球蓝细菌基因组被比较.先用BLASTP两两最佳匹配搜索和基因位置保守性的方法预测直系同源基因,并进一步计算每个基因组中所有基因在其他4种绿球蓝细菌基因组中直系同源基因的数量,从而达到识别外源基因的目的;而基因组岛是指由1个或几个连续外源基因组成的区域.结果表明:在5个绿球蓝细菌基因组中共识别出了16至52个不等的基因组岛,它们中的许多基因在病毒基因组或富含病毒的环境样本中都有同源基因.以上这些结果显示,在绿球蓝细菌中,病毒可能介导了基因的水平转移,从而导致了基因组的多样性.
위탐색수평기인전이재록구람세균기인조다양성중적작용,5개고광괄응성록구람세균기인조피비교.선용BLASTP량량최가필배수색화기인위치보수성적방법예측직계동원기인,병진일보계산매개기인조중소유기인재기타4충록구람세균기인조중직계동원기인적수량,종이체도식별외원기인적목적;이기인조도시지유1개혹궤개련속외원기인조성적구역.결과표명:재5개록구람세균기인조중공식별출료16지52개불등적기인조도,타문중적허다기인재병독기인조혹부함병독적배경양본중도유동원기인.이상저사결과현시,재록구람세균중,병독가능개도료기인적수평전이,종이도치료기인조적다양성.
In order to explore the role of horizontal gene transfer in the divergence of genomes within the genus Prochlorococcus, the genomes of five high light-adapted strains of Prochlorococcus were compared. Firstly, both bidirectional best BLASTP search and the conservation of gene order against each other were employed to predict orthologs;then the alien genes in one Prochlorococcus strain were identified by examining how many orthologs its each gene had in the genomes of the four other strains. As for a genomic island, it refers to a region composed of one or more contiguous alien genes. The results showed that the number of genomic islands in the five genomes ranged from 16 to 52. Many of the genes in these islands showed similarity to genes found in viral genomes or sequences derived from virus-enriched environmental samples. These results suggest that viruses may have mediated horizontal gene transfer, resulting in genomic divergence amongst Prochlorococcus genomes.