国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2008年
6期
325-328
,共4页
杨子峰%朱宇同%张奉学%杨瑞仪%刘妮%赵昉
楊子峰%硃宇同%張奉學%楊瑞儀%劉妮%趙昉
양자봉%주우동%장봉학%양서의%류니%조방
干扰素类%抗病毒药%冠状病毒
榦擾素類%抗病毒藥%冠狀病毒
간우소류%항병독약%관상병독
Interferon%Antiviral medicine%Coronavirus
目的 制备人ω干扰素(hIFN-ω)并初步研究其体外抗普通冠状病毒活性.方法 制备hIFN-ω cDNA,并在大肠杆菌BL-21中高效表达、纯化后,测定了hIFN一ω的抗滤泡口膜炎病毒(VSV)和冠状病毒昆徐株活性.结果 成功地获得了hIFN-ω GST融合蛋白,利用WISH-VSV系统检测hIFN-ω的抗病毒活力为7.81×104 kU/L;与基因工程α干扰素(IFN-a2b)的比较发现,hIFN-ω可以明显抑制冠状病毒昆徐株的活性,而IFN-α2b未见此活性.结论 hIFN-ω具有抑制冠状病毒昆徐株的活性,有独特的抗呼吸道病毒作用;不同的干扰素亚型对不同的病毒敏感性有所差异.
目的 製備人ω榦擾素(hIFN-ω)併初步研究其體外抗普通冠狀病毒活性.方法 製備hIFN-ω cDNA,併在大腸桿菌BL-21中高效錶達、純化後,測定瞭hIFN一ω的抗濾泡口膜炎病毒(VSV)和冠狀病毒昆徐株活性.結果 成功地穫得瞭hIFN-ω GST融閤蛋白,利用WISH-VSV繫統檢測hIFN-ω的抗病毒活力為7.81×104 kU/L;與基因工程α榦擾素(IFN-a2b)的比較髮現,hIFN-ω可以明顯抑製冠狀病毒昆徐株的活性,而IFN-α2b未見此活性.結論 hIFN-ω具有抑製冠狀病毒昆徐株的活性,有獨特的抗呼吸道病毒作用;不同的榦擾素亞型對不同的病毒敏感性有所差異.
목적 제비인ω간우소(hIFN-ω)병초보연구기체외항보통관상병독활성.방법 제비hIFN-ω cDNA,병재대장간균BL-21중고효표체、순화후,측정료hIFN일ω적항려포구막염병독(VSV)화관상병독곤서주활성.결과 성공지획득료hIFN-ω GST융합단백,이용WISH-VSV계통검측hIFN-ω적항병독활력위7.81×104 kU/L;여기인공정α간우소(IFN-a2b)적비교발현,hIFN-ω가이명현억제관상병독곤서주적활성,이IFN-α2b미견차활성.결론 hIFN-ω구유억제관상병독곤서주적활성,유독특적항호흡도병독작용;불동적간우소아형대불동적병독민감성유소차이.
Objective To obtain the human interferon-ω and observe its anti-coronavirus activities.Methods The cDNA of human interferion-ω was obtained,and the IFN-ω fusion protein was expressed and purified in Escherichla colt(E.eoli)BL-21.The anti-virus activities of human interferon-ω were accessed bv using WISH-VSV system,and then compared with those of IFN-a2b by using coronavirus(Kunxu strain).Results GST-IFN-ω fusion protein was obtained successfully.And the anti-virus activity of IFN-ω was 7.81×104 kU/L,which was accessed by WISH-VSV system.Compared with IFN-α2b,IFN-ω inhibited cytopathic effect of coronavirus(Kunxu strain),and IFN-α2b had no such effect.Conclusions IFN-ωcould inhibit coronavirus，indicating that in may have special way on anti-respirovirus.Also sensibilities were different among interferon subtypes.
