中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2011年
12期
924-927
,共4页
刘远梅%毛羽晨%金祝%周涛%高明娟
劉遠梅%毛羽晨%金祝%週濤%高明娟
류원매%모우신%금축%주도%고명연
肛管,畸形%直肠,畸形%神经生长因子%模型,动物
肛管,畸形%直腸,畸形%神經生長因子%模型,動物
항관,기형%직장,기형%신경생장인자%모형,동물
Anal canal,abnormalities%Rectum,abnormalities%Nerve growth factor%Models,animal
目的 观察肛门直肠畸形(ARM)动物模型直肠末端组织中神经生长因子(NGF)及其受体(NGFR)的表达,探讨ARM排便功能障碍的机制.方法 应用免疫组织化学和RT-PCR检测正常与肛门直肠畸形动物胎鼠直肠末端组织中NGF及其高亲和力受体(TrkA)、低亲和力受体(p75NTR)的表达.结果 NGF、TrkA、p75NTR在正常胎鼠直肠末端肠黏膜皱襞及肌层细胞质及细胞核中有大量表达,其平均光密度(IOD值)分别为731.749±232.177,262.815±101.722,134.674±67.127,而在肛门直肠畸形直肠末端表达较少,其平均光密度(IOD值)分别为17.973±74.945,33.145±11.128,113.010±36.468,两组比较,差异均有统计学意义(P＜0.05)；TrkAmRNA、P75NTRmRNA在正常胎鼠直肠末端组织中的相对表达量分别为162.221±104.675,129.778±51.967,在肛门直肠畸形胎鼠中的相对表达量分别为78.973±33.425,87.145±25.812,两组比较,差异有统计学意义(P＜0.05).结论 NGF及其受体TrkA、p75NTR在ARM动物模型直肠末端的表达异常可能是导致其排便功能障碍的原因之一.
目的 觀察肛門直腸畸形(ARM)動物模型直腸末耑組織中神經生長因子(NGF)及其受體(NGFR)的錶達,探討ARM排便功能障礙的機製.方法 應用免疫組織化學和RT-PCR檢測正常與肛門直腸畸形動物胎鼠直腸末耑組織中NGF及其高親和力受體(TrkA)、低親和力受體(p75NTR)的錶達.結果 NGF、TrkA、p75NTR在正常胎鼠直腸末耑腸黏膜皺襞及肌層細胞質及細胞覈中有大量錶達,其平均光密度(IOD值)分彆為731.749±232.177,262.815±101.722,134.674±67.127,而在肛門直腸畸形直腸末耑錶達較少,其平均光密度(IOD值)分彆為17.973±74.945,33.145±11.128,113.010±36.468,兩組比較,差異均有統計學意義(P＜0.05)；TrkAmRNA、P75NTRmRNA在正常胎鼠直腸末耑組織中的相對錶達量分彆為162.221±104.675,129.778±51.967,在肛門直腸畸形胎鼠中的相對錶達量分彆為78.973±33.425,87.145±25.812,兩組比較,差異有統計學意義(P＜0.05).結論 NGF及其受體TrkA、p75NTR在ARM動物模型直腸末耑的錶達異常可能是導緻其排便功能障礙的原因之一.
목적 관찰항문직장기형(ARM)동물모형직장말단조직중신경생장인자(NGF)급기수체(NGFR)적표체,탐토ARM배편공능장애적궤제.방법 응용면역조직화학화RT-PCR검측정상여항문직장기형동물태서직장말단조직중NGF급기고친화력수체(TrkA)、저친화력수체(p75NTR)적표체.결과 NGF、TrkA、p75NTR재정상태서직장말단장점막추벽급기층세포질급세포핵중유대량표체,기평균광밀도(IOD치)분별위731.749±232.177,262.815±101.722,134.674±67.127,이재항문직장기형직장말단표체교소,기평균광밀도(IOD치)분별위17.973±74.945,33.145±11.128,113.010±36.468,량조비교,차이균유통계학의의(P＜0.05)；TrkAmRNA、P75NTRmRNA재정상태서직장말단조직중적상대표체량분별위162.221±104.675,129.778±51.967,재항문직장기형태서중적상대표체량분별위78.973±33.425,87.145±25.812,량조비교,차이유통계학의의(P＜0.05).결론 NGF급기수체TrkA、p75NTR재ARM동물모형직장말단적표체이상가능시도치기배편공능장애적원인지일.
Objective To study the expression of nerve growth factor (NGF) and nerve growth factor receptor (NGFR) in distal rectum in fetal rats with anorectal malformations.Methods The intragastric administration of ethylenethiourea was performed in fetal rats to create anorectal malformation model.The expression level of NGF,TrkA and p75NTR of distal rectum tissues in fetal rats were studied by using immunohistochemistry and RT-PCR.Results In normal control group,the average IOD value of NGF and TrkA,as well as p75NTR were respectively 731.749 ± 232.177,262.815 ± 101.722 and 134.674 ± 67.127,while in induced ARM group they were 17.973 ± 74.945、33.145 ± 11.128、113.010 ± 36.468 respectively.There are significant differences between both groups(P＜0.05).In term of mRNA relative expression of TrkA and P75NTR,they also presented remarkably statistical differences via comparison (Normal control group:162.221 ± 104.675 and 129.778 ± 51.967; while the induced ARM group:78.973 ± 33.425 and 87.145 ± 25.812) (P＜ 0.05).Conclusions The abnormal expression of NGF and NGFR,might be one of the pathogenesis that plays important role in defecation functional perturbation in fetal ARM rats.
