CAJ | 학술논문

目的探讨麝香保心丸对心肌梗死后心力衰竭大鼠血清Th1型细胞因子、肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)和Th2型细胞因子白介素-4(IL-4)、白介索-10(IL-10)水平的影响.方法结扎大鼠左冠状动脉前降支制作心肌梗死后心力衰竭模型,54只Wistar大鼠随机分为正常对照组、假手术组、心力衰竭模型对照组(模型组)、麝香保心丸小剂量组(小剂量组)、麝香保心丸大剂量组(大剂量组)和阳性药物对照组(阳性对照组),采用灌胃法给药.超声心动图检测用药前后大鼠心功能,放射免疫法测定血清TNF-α、IL-10水平,酶联免疫吸附法测定血清IFN-γ、IL-4水平.结果用药前,假手术组与正常对照组左心室射血分数(LVEF)比较,差异无统计学意义;与正常对照组和假手术组比较,模型组、小剂量组大剂量组和阳性对照组大鼠LVEF降低(均P＜0.01);用药后,与假手术组比较,模型组的TNF-α和IFN-γ水平升高(P＜0.01);IL-4和IL-10水平降低(P＜0.01);与模型组比较,大剂量组的LVEF改善(P＜0.05),TNF-α和IFN-γ水平降低(P＜0.05,P＜0.01),IL-4和IL-10水平升高(P＜0.05),小剂量组的IL-10水平升高(P＜0.05),IFN-γ水平下降(P＜0.01);阳性对照组IL-10水平升高(P＜0.05),TNF-α和IFN-γ水平下降(P＜0.05,P＜0.01);小剂量组和阳性对照组的IL-4水平有升高趋势,LVEF有下降趋势,但差异均无统计学意义.结论麝香保心丸治疗改善了心肌梗死后心力衰竭大鼠的左心功能,大鼠血清IL-4、IL-10水平升高,TNF-α、IFN-γ降低.
목적 탐토사향보심환대심기경사후심력쇠갈대서혈청Th1형세포인자、종류배사인자-α(TNF-α)、간우소-γ(IFN-γ)화Th2형세포인자백개소-4(IL-4)、백개색-10(IL-10)수평적영향.방법 결찰대서좌관상동맥전강지제작심기경사후심력쇠갈모형,54지Wistar대서수궤분위정상대조조、가수술조、심력쇠갈모형대조조(모형조)、사향보심환소제량조(소제량조)、사향보심환대제량조(대제량조)화양성약물대조조(양성대조조),채용관위법급약.초성심동도검측용약전후대서심공능,방사면역법측정혈청TNF-α、IL-10수평,매련면역흡부법측정혈청IFN-γ、IL-4수평.결과 용약전,가수술조여정상대조조좌심실사혈분수(LVEF)비교,차이무통계학의의;여정상대조조화가수술조비교,모형조、소제량조대제량조화양성대조조대서LVEF강저(균P＜0.01);용약후,여가수술조비교,모형조적TNF-α화IFN-γ수평승고(P＜0.01);IL-4화IL-10수평강저(P＜0.01);여모형조비교,대제량조적LVEF개선(P＜0.05),TNF-α화IFN-γ수평강저(P＜0.05,P＜0.01),IL-4화IL-10수평승고(P＜0.05),소제량조적IL-10수평승고(P＜0.05),IFN-γ수평하강(P＜0.01);양성대조조IL-10수평승고(P＜0.05),TNF-α화IFN-γ수평하강(P＜0.05,P＜0.01);소제량조화양성대조조적IL-4수평유승고추세,LVEF유하강추세,단차이균무통계학의의.결론 사향보심환치료개선료심기경사후심력쇠갈대서적좌심공능,대서혈청IL-4、IL-10수평승고,TNF-α、IFN-γ강저.
Objective To investigate the effects of Shexiangbaoxin pills (SXBXP) on the serum levels of T helper type 1 (Th1) cytokines including tumor necrosis factor-a (TNF-α),interferon-γ(IFN-γ) and T helper type 2 (Th2) cytokines incloding interleukin-4 (IL-4),interleukin-10 (IL-10)in rats with chronic heart failure (CHF).Methods The CHF models were established by left anterior descending coronary artery ligation.Fifty-four Wistar rats were randomly divided into six groups:control group,sham operation group,CHF model group,small dose SXBXP group,large dose SXBXP group and positive medicine group.Heart function was examined by echocardiography before and after therapy,the TNF-α and IL-10 levels were measured by radioimmunoassay,IFN-γ and IL-4 levels were measured by enzyme linked immunosorbent assay (ELISA).Results (1) Before therapy,there was no significant difference between control group and sham operation group in ejection fraction of left ventricle (LVEF).Compared with control group and sham operation group,the levels of LVEF were significantly decreased in CHF model group,small dose SXBXP group,large dose SXBXP group and positive medicine group (all P＜0.01).(2) After therapy,compared with sham operation group,the serum levels of TNF-α and IFN-γ were markedly increased,and IL-4 and IL-10 were significantly decreased in CHF model group (all P＜0.01 ).Compared with CHF model group,LVEF was significantly improved,serum levels of TNF-α and IFN-γ were markedly decreased,IL-4 and IL-10 were markedly increased in large dose SXBXP group (all P＜0.05).In small dose SXBXP group,serum levels of IFN-γ were markedly decreased and IL-10 were markedly increased (all P＜0.05).In positive medicine group,serum levels of TNF-α and IFN-γ were markedly decreased and IL-10 were markedly increased (all P＜0.05).In small dose SXBXP group and positive medicine group,serum levels of IL-4 increased and LVEF decreased,even though there was no significant difference compared with CHF model group.Conclusions The SXBXP therapy of rats with CHF induced by myocardial infarction improves the heart function,decreases the serum levels of TNF-α and IFN-γ,and increases serum levels of IL-4 and IL-10.