中华结核和呼吸杂志
中華結覈和呼吸雜誌
중화결핵화호흡잡지
Chinese Journal of Tuberculosis and Respiratory Diseases
2012年
2期
120-124
,共5页
张念荣%范刚%邓云峰%王新锋%路超%张春芝%董振芳%张健%李丽%赵胜梅%卢志明
張唸榮%範剛%鄧雲峰%王新鋒%路超%張春芝%董振芳%張健%李麗%趙勝梅%盧誌明
장념영%범강%산운봉%왕신봉%로초%장춘지%동진방%장건%리려%조성매%로지명
HLA抗原%结核,肺%聚合酶链反应%疾病遗传易感性
HLA抗原%結覈,肺%聚閤酶鏈反應%疾病遺傳易感性
HLA항원%결핵,폐%취합매련반응%질병유전역감성
HLA antigens%Tuberculosis,pulmonary%Polymerase chain reaction%Genetic predisposition to disease
目的 分析人白细胞抗原(HLA) -Cw基因多态性与肺结核遗传易感性的关系,探究肺结核的易感基因和抵抗基因.方法 选取2010年5月至2011年5月山东省胸科医院确诊的继发性肺结核患者112例为肺结核组,其中男62例,女50例；年龄19 ～ 69岁,平均41.7岁；根据痰涂片抗酸染色结果分为涂阴组77例和涂阳组35例.对照组为同期在该院健康体检的110名,其中男59名,女51名；年龄21 ～ 67岁,平均38.3岁.提取基因组DNA,扩增HLA-Cw等位基因,进行Hardy-Weinberg吻合度检验,估算基因频率(％)=1 -(1-表型频率)1/2.两组间比较采用x2检验,检验水准α/8=0.00625,P＜0.00625为差异有统计学意义.结果 肺结核组HLA-Cw08等位基因的基因频率(43.6％,75/112)显著高于对照组(27.4％,52/110),差异有统计学意义(x2=8.790,P＜0.00625)；肺结核涂阳组HLA-Cw04等位基因的基因频率(20.7％,13/35)显著高于涂阴组(4.7％,7/77),HLA-Cw08等位基因的基因频率(22.5％,14/35)显著低于涂阴组(54.4％,61/77),差异均有统计学意义(x2值分别为12.909和16.732,均P＜0.00625).结论 HLA-Cw基因多态性与肺结核有一定相关性,HLA-Cw08可能是肺结核的易感基因,HLA-Cw04和08等位基因可能与MTB的感染状态和结局有关.
目的 分析人白細胞抗原(HLA) -Cw基因多態性與肺結覈遺傳易感性的關繫,探究肺結覈的易感基因和牴抗基因.方法 選取2010年5月至2011年5月山東省胸科醫院確診的繼髮性肺結覈患者112例為肺結覈組,其中男62例,女50例；年齡19 ～ 69歲,平均41.7歲；根據痰塗片抗痠染色結果分為塗陰組77例和塗暘組35例.對照組為同期在該院健康體檢的110名,其中男59名,女51名；年齡21 ～ 67歲,平均38.3歲.提取基因組DNA,擴增HLA-Cw等位基因,進行Hardy-Weinberg吻閤度檢驗,估算基因頻率(％)=1 -(1-錶型頻率)1/2.兩組間比較採用x2檢驗,檢驗水準α/8=0.00625,P＜0.00625為差異有統計學意義.結果 肺結覈組HLA-Cw08等位基因的基因頻率(43.6％,75/112)顯著高于對照組(27.4％,52/110),差異有統計學意義(x2=8.790,P＜0.00625)；肺結覈塗暘組HLA-Cw04等位基因的基因頻率(20.7％,13/35)顯著高于塗陰組(4.7％,7/77),HLA-Cw08等位基因的基因頻率(22.5％,14/35)顯著低于塗陰組(54.4％,61/77),差異均有統計學意義(x2值分彆為12.909和16.732,均P＜0.00625).結論 HLA-Cw基因多態性與肺結覈有一定相關性,HLA-Cw08可能是肺結覈的易感基因,HLA-Cw04和08等位基因可能與MTB的感染狀態和結跼有關.
목적 분석인백세포항원(HLA) -Cw기인다태성여폐결핵유전역감성적관계,탐구폐결핵적역감기인화저항기인.방법 선취2010년5월지2011년5월산동성흉과의원학진적계발성폐결핵환자112례위폐결핵조,기중남62례,녀50례；년령19 ～ 69세,평균41.7세；근거담도편항산염색결과분위도음조77례화도양조35례.대조조위동기재해원건강체검적110명,기중남59명,녀51명；년령21 ～ 67세,평균38.3세.제취기인조DNA,확증HLA-Cw등위기인,진행Hardy-Weinberg문합도검험,고산기인빈솔(％)=1 -(1-표형빈솔)1/2.량조간비교채용x2검험,검험수준α/8=0.00625,P＜0.00625위차이유통계학의의.결과 폐결핵조HLA-Cw08등위기인적기인빈솔(43.6％,75/112)현저고우대조조(27.4％,52/110),차이유통계학의의(x2=8.790,P＜0.00625)；폐결핵도양조HLA-Cw04등위기인적기인빈솔(20.7％,13/35)현저고우도음조(4.7％,7/77),HLA-Cw08등위기인적기인빈솔(22.5％,14/35)현저저우도음조(54.4％,61/77),차이균유통계학의의(x2치분별위12.909화16.732,균P＜0.00625).결론 HLA-Cw기인다태성여폐결핵유일정상관성,HLA-Cw08가능시폐결핵적역감기인,HLA-Cw04화08등위기인가능여MTB적감염상태화결국유관.
Objective To analyze the relationship between HLA-Cw polymorphism and susceptibility to pulmonary tuberculosis ( PTB),and therefore to explore the susceptible or resistant genes of PTB.Methods A hundred and twelve patients who were confirmed to have secondary PTB in Shandong Chest Hospital from May 2010 to May 2011 were selected as the PTB group,including 62 males and 50 females aged 19-69 years (mean 41.7).According to the acid-fast staining results,PTB patients were divided into a smear-negative group (SN group,77 cases) and a smear-positive group (SP group,35 cases).A hundred and ten subjects who underwent physical examination in Shandong Chest Hospital at the same period were selected as the control group,including 59 males and 51 females aged 21 - 67 years ( mean 38.3).After genomic DNA was extracted,genotyping of HLA-Cw was conducted by sequence specific primer polymerase chain reaction (PCR-SSP) method.Then Hardy-Weinberg (H-W) equilibrium was tested,and gene frequencies ( ％ ) were estimated =1 - ( 1 - phenotype frequencies) 1/2.Gene frequencies were compared between the PTB group and the control group,and between the SN group and SP group by x2 test.According to Bonferroni' s principle,α was divided by the number of alleles ( n =8 ),and P ＜0.00625 was regarded as statistically significant.Results The frequency of HLA-Cw08 was significantly higher in PTB patients (43.6％,75/112) compared with the controls (27.4％,52/110),x2 =8.790,P ＜ 0.006 25. Among PTB patients,HLA-Cw04 had a significantly higher frequency in the SP group (20.7％,13/35) than in the SN group (4.7％,7/77),while HLA-Cw08 had a significantly lower frequency in the SP group (22.5％,14/35) than in the SN group (54.4％,61/77),x2 =12.909,16.732,both P ＜ 0.006 25.Conclusions HLA-Cw polymorphism is related to susceptibility to PTB.HLA-Cw08 may be one of the susceptible genes for PTB,and HLA-Cw04 and 08 may be related to MTB infectious status and clinical outcomes.
