CAJ | 학술논문

目的探讨胃镜冷光源照射对美蓝(MB)染色后的胃癌细胞DNA的损伤情况,并探索损伤的发生机制和作用规律.方法应用单细胞凝胶电泳技术,检测DNA损伤情况;Annexin VFITC/PI双染后流式细胞仪检测细胞凋亡;采用荧光探针DCFH-DA,进行细胞内活性氧(ROS)检测.结果冷光照射MB染色的SGC7901细胞后,其DNA损伤程度较未照射组增加(F=8.39,P＜0.05),损伤严重程度与光照时间呈正相关,光照停止则有损伤修复,照射后细胞较未照射细胞出现明显凋亡(x2=7.71,P＜0.05);同时,经照射后的细胞内ROS高于未照射组(F=34.11,P＜0.01).结论美蓝色素内镜的冷光辐射可造成SGC7901细胞的DNA损伤,并诱发细胞凋亡,其作用机制与光化学反应激发产生的过量ROS有关.
목적 탐토위경랭광원조사대미람(MB)염색후적위암세포DNA적손상정황,병탐색손상적발생궤제화작용규률.방법 응용단세포응효전영기술,검측DNA손상정황;Annexin VFITC/PI쌍염후류식세포의검측세포조망;채용형광탐침DCFH-DA,진행세포내활성양(ROS)검측.결과 랭광조사MB염색적SGC7901세포후,기DNA손상정도교미조사조증가(F=8.39,P＜0.05),손상엄중정도여광조시간정정상관,광조정지칙유손상수복,조사후세포교미조사세포출현명현조망(x2=7.71,P＜0.05);동시,경조사후적세포내ROS고우미조사조(F=34.11,P＜0.01).결론 미람색소내경적랭광복사가조성SGC7901세포적DNA손상,병유발세포조망,기작용궤제여광화학반응격발산생적과량ROS유관.
Objective To study the co-effects of methylene blue(MB) and exposure with the cold light source on cell DNA damage, and to explore the mechanism involved. Methods The alkaline single cell gel electrophoresis was used to determine cell DNA damage. Apoptosis of the cells was determined by flow cytometry analysis using Annexin V-FITC/PI staining. DCFH-DA probe was used to determine endocellular Reactive Oxygen Species (ROS). Results The levels of DNA damage in the SGC7901 adenocarcinoma cells treated with methylene blue in the light were significantly increased compared to that of control ( F = 8.39, P＜0.05 ). The DNA damage levels were related to the length of time of light exposure, and the damage was recovered to a certain level after light withdrew. Cell apoptosis ( x2=7.71,P ＜0.05)and endocellular ROS level (F = 34.11, P＜0.01＝ increased significantly in the exposure group. Conclusions Methylene blue chromoendoscopy can induce DNA damage and cell apoptosis, and the mechanism may be associated with ROS produced by the photochemical reaction.