中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2011年
12期
755-758
,共4页
陈东平%裴丹丹%王亚珂%黄翠%阿达莱提·麻合苏提%刘思颖
陳東平%裴丹丹%王亞珂%黃翠%阿達萊提·痳閤囌提%劉思穎
진동평%배단단%왕아가%황취%아체래제·마합소제%류사영
牙本质黏结剂%疏水性%乙醇%微渗透
牙本質黏結劑%疏水性%乙醇%微滲透
아본질점결제%소수성%을순%미삼투
Dentin-bonding agents%Hydrophobicity%Ethanol%Micropermeability
目的 研究牙本质疏水性粘接前乙醇润湿对粘接强度和粘接界面渗透性的影响,以期更好地为临床提供指导.方法 24颗无龋第三磨牙去除冠部釉质后暴露中层牙本质,利用随机数字表随机分成6个组(每组4颗).所有操作过程均在模拟髓腔压力的情况下进行,对照组采用全酸蚀粘接剂( Adper Scotchbond Multi-Purpose)粘接；4个实验组分别用无水乙醇润湿牙面20 s、1min、2 min、3min,1个实验组使用梯度乙醇润湿牙面,5个实验组采用实验室配制的疏水性粘接剂粘接.固化24h后进行微拉伸强度检测,计算粘接强度.荧光染料罗丹明B于牙髓腔中荷载3h后采用激光扫描共聚焦显微镜观察粘接界面的渗透情况.结果 与对照组相比,乙醇润湿20 s组和1min组粘接强度[(21.02±7.23) MPa和(29.64±3.81) MPa]均显著低于对照组[(38.14±4.97) MPa],差异有统计学意义(P＞0.05)；而乙醇润湿2 min组[(38.40 ±5.03) MPa]、3min组[(37.26±4.68)MPa]和梯度乙醇润湿组[(40.12±5.95) MPa]的粘接强度与对照组接近,差异无统计学意义(P＜0.05).激光扫描共聚焦显微镜观察显示,随着乙醇润湿时间的延长,荧光染料在混合层和牙本质小管侧壁的沉积逐渐减轻,其中梯度乙醇润湿组仅在混合层附近检测到少量荧光染料.结论 在模拟髓腔压力的情况下,2 min以上的乙醇润湿结合疏水性粘接剂可获得良好的粘接力,同时明显降低粘接界面的微渗透.
目的 研究牙本質疏水性粘接前乙醇潤濕對粘接彊度和粘接界麵滲透性的影響,以期更好地為臨床提供指導.方法 24顆無齲第三磨牙去除冠部釉質後暴露中層牙本質,利用隨機數字錶隨機分成6箇組(每組4顆).所有操作過程均在模擬髓腔壓力的情況下進行,對照組採用全痠蝕粘接劑( Adper Scotchbond Multi-Purpose)粘接；4箇實驗組分彆用無水乙醇潤濕牙麵20 s、1min、2 min、3min,1箇實驗組使用梯度乙醇潤濕牙麵,5箇實驗組採用實驗室配製的疏水性粘接劑粘接.固化24h後進行微拉伸彊度檢測,計算粘接彊度.熒光染料囉丹明B于牙髓腔中荷載3h後採用激光掃描共聚焦顯微鏡觀察粘接界麵的滲透情況.結果 與對照組相比,乙醇潤濕20 s組和1min組粘接彊度[(21.02±7.23) MPa和(29.64±3.81) MPa]均顯著低于對照組[(38.14±4.97) MPa],差異有統計學意義(P＞0.05)；而乙醇潤濕2 min組[(38.40 ±5.03) MPa]、3min組[(37.26±4.68)MPa]和梯度乙醇潤濕組[(40.12±5.95) MPa]的粘接彊度與對照組接近,差異無統計學意義(P＜0.05).激光掃描共聚焦顯微鏡觀察顯示,隨著乙醇潤濕時間的延長,熒光染料在混閤層和牙本質小管側壁的沉積逐漸減輕,其中梯度乙醇潤濕組僅在混閤層附近檢測到少量熒光染料.結論 在模擬髓腔壓力的情況下,2 min以上的乙醇潤濕結閤疏水性粘接劑可穫得良好的粘接力,同時明顯降低粘接界麵的微滲透.
목적 연구아본질소수성점접전을순윤습대점접강도화점접계면삼투성적영향,이기경호지위림상제공지도.방법 24과무우제삼마아거제관부유질후폭로중층아본질,이용수궤수자표수궤분성6개조(매조4과).소유조작과정균재모의수강압력적정황하진행,대조조채용전산식점접제( Adper Scotchbond Multi-Purpose)점접；4개실험조분별용무수을순윤습아면20 s、1min、2 min、3min,1개실험조사용제도을순윤습아면,5개실험조채용실험실배제적소수성점접제점접.고화24h후진행미랍신강도검측,계산점접강도.형광염료라단명B우아수강중하재3h후채용격광소묘공취초현미경관찰점접계면적삼투정황.결과 여대조조상비,을순윤습20 s조화1min조점접강도[(21.02±7.23) MPa화(29.64±3.81) MPa]균현저저우대조조[(38.14±4.97) MPa],차이유통계학의의(P＞0.05)；이을순윤습2 min조[(38.40 ±5.03) MPa]、3min조[(37.26±4.68)MPa]화제도을순윤습조[(40.12±5.95) MPa]적점접강도여대조조접근,차이무통계학의의(P＜0.05).격광소묘공취초현미경관찰현시,수착을순윤습시간적연장,형광염료재혼합층화아본질소관측벽적침적축점감경,기중제도을순윤습조부재혼합층부근검측도소량형광염료.결론 재모의수강압력적정황하,2 min이상적을순윤습결합소수성점접제가획득량호적점접력,동시명현강저점접계면적미삼투.
Objective To evaluate the micropermeability on bonding hydrophobic adhesive to dentin with ethanol-wet bonding under simulated pulp pressure.Methods Twenty-four intact human third molars were used in the study.After the enamel of occlusal surfaces was removed,the molars were randomly divided into six groups.Adper Scotchbond Multi-Purpose was used in the control group; in the experimental groups,the dentin surfaces were saturated with ethanol for 20 s ( group 1 ),1 min ( group 2 ),2 min ( group 3 ),3 min ( group 4 ) or with a series of increasing ethanol concentrations before application of hydrophobic adhesive ( group 5 ).All the bonding procedures were done under simulated pulp pressure.After 24 hours,micro-tensile bond strength test were performed on the specimens.Bonding interfaces were observed under laser scanning confocal microscope (LSCM) after the pulp chamber were filled with a water-soluble fluoroprobe rhodamine B for 3 hours.Results Compared with the control group[(38.14 ± 4.97 ) MPa],bond strengths in group 1 [(21.02 ± 7.23 ) MPa]and group 2 [( 29.64 ± 3.81 ) MPa]were statistically lower ( P ＞ 0.05 ),while bond strngth in group 3 [( 38.40 ± 5.03 ) MPa],group 4 [( 37.26 ± 4.68 ) MPa]and group 5[(40.12 ±5.95) MPa]were similar to the control group (P＜0.05).The images taken by LSCM showed that with extension of ethanol-wet time,the deposition of fluorescent dye in hybrid layer and along the dentinal tubules decreased gradually.Especially in group 5,only spare fluorescent dye deposition could be detected in the hybrid layer.Conclusions Dentin saturated with ethanol for more than 2 min before bonding hydrophobic adhesive to dentin could provide favorable bond strength and decreased the micropermeability of bonding interfaces under simulated pulp pressure.
