CAJ | 학술논문

目的对新型含能材料二硝酰胺铵(ADN)的急性毒性、亚急性毒性和亚慢性毒性进行研究,确定ADN的急性毒性分级、毒作用性质及靶器官.方法根据《化学品毒性鉴定技术规范》,采用大鼠和小鼠急性经口毒性试验、亚急性经口(28d)毒性试验和亚慢性经口(90d)毒性试验.结果 (1)急性经口毒性试验结果表明,ADN对小鼠的经口半数致死剂量LD50为568.9 mg/kg,大鼠为616.6 mg/kg,急性毒性分级属低毒级化学物.(2)亚急性经口(28d)毒性试验结果表明,雌、雄鼠123 mg/kg剂量组体重增长明显低于对照组；61.6、123 mg/kg剂量组血清中总胆红素、天冬氨酸氨基转移酶活力明显高于对照组,肝/体比值明显低于对照组.(3)亚慢性经口(90d)毒性试验结果表明,从染毒第5周开始,123 mg/kg剂量组雌性大鼠体重增长幅度明显降低,与对照组相比,差异有统计学意义(P＜0.05)；61.6、123mg/kg剂量组血清中总胆红素、天冬氨酸氨基转移酶活力高于对照组,肝/体比值明显低于对照组,肝脏病变检出例数明显高于对照组,差异均有统计学意义(P＜0.05).结论 ADN急性毒性分级属低毒级,其无可见有害作用水平(NOAEL)为30.8 mg/kg,毒作用的靶器官主要为肝脏.
목적 대신형함능재료이초선알안(ADN)적급성독성、아급성독성화아만성독성진행연구,학정ADN적급성독성분급、독작용성질급파기관.방법 근거《화학품독성감정기술규범》,채용대서화소서급성경구독성시험、아급성경구(28d)독성시험화아만성경구(90d)독성시험.결과 (1)급성경구독성시험결과표명,ADN대소서적경구반수치사제량LD50위568.9 mg/kg,대서위616.6 mg/kg,급성독성분급속저독급화학물.(2)아급성경구(28d)독성시험결과표명,자、웅서123 mg/kg제량조체중증장명현저우대조조；61.6、123 mg/kg제량조혈청중총담홍소、천동안산안기전이매활력명현고우대조조,간/체비치명현저우대조조.(3)아만성경구(90d)독성시험결과표명,종염독제5주개시,123 mg/kg제량조자성대서체중증장폭도명현강저,여대조조상비,차이유통계학의의(P＜0.05)；61.6、123mg/kg제량조혈청중총담홍소、천동안산안기전이매활력고우대조조,간/체비치명현저우대조조,간장병변검출례수명현고우대조조,차이균유통계학의의(P＜0.05).결론 ADN급성독성분급속저독급,기무가견유해작용수평(NOAEL)위30.8 mg/kg,독작용적파기관주요위간장.
Objective To study the acute,subacute and subchronic toxicity induced by ammonium dinitramide (ADN),and to ascertain the gradation and target organs of acute toxicity induced by AND.Methods According to technical specifications for toxicity determination of chemicals,the oral tests for acute,subacute and subchronic toxicity induced by AND were performed for 90 days.Results The oral LD50 for mouse and rat was 568.9 mg/kg and 616.6 mg/kg ADN respectively.The gradation of acute toxicity induced by AND was low level.The results of oral subacute and subchronic toxicity tests (for 28 and 90 days) showed that a gain in weight in group exposed to 123 mg/kg AND was significantly lower than that in control group (P＜0.05),the TBIL and ALT in group exposed to 61.6 and 123 mg/kg AND significantly increased and the ratio of liver weight to body weight obviously decreased,as compared with control group,the number of animals with hepatic pathological changes in group exposed to 61.6 and 123 mg/kg AND was significantly higher than that in control group (P＜0.05).Conclusion The gradation of acute toxicity induced by ADN was low level.When the exposure dose of AND was 30.8mg/kg,the adverse effect was not observed,and the target organ was liver.