CAJ | 학술논문

目的建立眼前节内眼模拟手术诱发血眼屏障破坏的大鼠动物模型.方法清洁级健康成年雄性Sprague-Dawley大鼠150只,随机分为对照组和模型组,每组75只.按1 ml/kg的剂量腹腔注射盐酸氯胺酮-盐酸甲苯噻嗪混合液麻醉大鼠.磷酸盐缓冲液灌注袋连接三通管.三通管一端连接24G静脉留置针,手术显微镜下在3点时钟位从角巩缘前透明角膜30°斜行穿刺入前房,退出针头,留置套管；另一端连接24G静脉留置针套管,与测压计相连测量大鼠眼压.大鼠眼压波动于0～12 mm Hg(1 mm Hg=0.133 kPa)之间,波动30次/min,重复60次.采用氧氟沙星滴眼液滴眼.建模后第1、2、3、5、7天,采用免疫组织化学法检测大鼠白蛋白；定量检测大鼠房水、视网膜中伊凡思蓝(EB)浓度.结果免疫组织化学染色结果显示,建模后第1、2、3、5、7天对照组白蛋白阳性染色均局限于虹膜和视网膜血管内,脉络膜弥漫性着色.建模后第1天,模型组白蛋白阳性染色主要位于虹膜和视网膜神经层血管周围；建模后第2、3天,阳性染色扩散到虹膜和视网膜全层；建模后第5、7天,阳性染色主要局限于虹膜和视网膜血管内.模型组房水中EB浓度在建模后第1、2、3、5天,均较对照组高(t=25.781,37.433,25.150,19.171；P＜0.01)；建模后第7天,与对照组接近(t=1.303,P=0.209).模型组视网膜EB浓度在建模后第1、2、3天,均较对照组高(t=11.997,14.622,23.014；P＜0.01)；建模后第5、7天,与对照组接近(t=2.027,0.756; P=0.058,0.459).结论通过模拟眼前节内眼手术损伤因素,可建立内眼手术诱发的血眼屏障破坏的大鼠动物模型.
목적 건립안전절내안모의수술유발혈안병장파배적대서동물모형.방법 청길급건강성년웅성Sprague-Dawley대서150지,수궤분위대조조화모형조,매조75지.안1 ml/kg적제량복강주사염산록알동-염산갑분새진혼합액마취대서.린산염완충액관주대련접삼통관.삼통관일단련접24G정맥류치침,수술현미경하재3점시종위종각공연전투명각막30°사행천자입전방,퇴출침두,류치투관；령일단련접24G정맥류치침투관,여측압계상련측량대서안압.대서안압파동우0～12 mm Hg(1 mm Hg=0.133 kPa)지간,파동30차/min,중복60차.채용양불사성적안액적안.건모후제1、2、3、5、7천,채용면역조직화학법검측대서백단백；정량검측대서방수、시망막중이범사람(EB)농도.결과 면역조직화학염색결과현시,건모후제1、2、3、5、7천대조조백단백양성염색균국한우홍막화시망막혈관내,맥락막미만성착색.건모후제1천,모형조백단백양성염색주요위우홍막화시망막신경층혈관주위；건모후제2、3천,양성염색확산도홍막화시망막전층；건모후제5、7천,양성염색주요국한우홍막화시망막혈관내.모형조방수중EB농도재건모후제1、2、3、5천,균교대조조고(t=25.781,37.433,25.150,19.171；P＜0.01)；건모후제7천,여대조조접근(t=1.303,P=0.209).모형조시망막EB농도재건모후제1、2、3천,균교대조조고(t=11.997,14.622,23.014；P＜0.01)；건모후제5、7천,여대조조접근(t=2.027,0.756; P=0.058,0.459).결론 통과모의안전절내안수술손상인소,가건립내안수술유발적혈안병장파배적대서동물모형.
Objective To establish a rat model of blood ocular barrier breakdown induced by anterior segment intraocular analogic surgery. Methods One hundred and fifty healthy adult male rats were randomly divided into control group and model group,75 rats in each group.The rats were anesthetized with 1 ml/kg ketamine hydrochloride/xylazine hydrochloride solution.Three way pipes were attached to a phosphate buffer infusion bag and two intravenous catheters. One catheter was inserted 30° obliquelythrough the transparent cornea anterior to the limbus into the rat's anterior chamber.Then the needle was withdrawn and the sheath was indwelling.Another catheter was connected with a manometer.Intraocular pressure was varied from 0 to 12 mm Hg (1 mm Hg=0.133 kPa) 60 times,30 times per min.The catheter was removed.The eyes were treated with ofloxacin ophthalmic solution after surgery.The 1st,2nd,3rd,5th and 7th day after surgery,the integrity of the blood ocular barrier was assessed by immunohistochemical staining for albumin and quantitative measurement using Evan's blue as a tracer. Results Albumin immunohistochemical staining of the control group was confined to the iris and retinal blood vessels.The choroid was stained at each time point after surgery.Albumin immunohistochemical staining of the model group was abundant around the iris and the retinal vasculature on the 1st day after surgery.The albumin diffused throughout the iris and the retina on the 2nd and the 3rd day after surgery.The albumin reached the retinal vessels on the 5th and 7th day after surgery.The aqueous humor Evans blue leakages of the model group were higher than those of the control group on the 1st,2nd,3rd and 5th day after surgery.The differences were statistically significant (t=25.781,37.433,25.150,19.171; P＜0.01).The Evans blue leakage of the model group was close to that of the control group on the 7th day after surgery. The difference was no statistical significant(t=1.303,P=0.209).The retinal Evans blue leakages of the model group were higher than those of the control group on the 1st,the 2nd and the 3rd day after surgery.The differences were statistically significant (t=11.997,14.622,23.014; P＜0.01).The Evans blue leakage of the model group was close to those of the control group on the 5th and 7th day after surgery. The differences were not statistically significant(t=2.027,0.756 ； P=0.058,0.459).Conclusion This study establishes a rat model of blood ocular barrier breakdown induced by imitating the injury to the anterior segment during intraocular surgery.