CAJ | 학술논문

目的测定中国狂犬病毒人用疫苗株aG和减毒株CTN181的RdRp编码(L)基因序列并初步研究其结构和功能特点.方法 RT-PCR法获得若干交叉重叠小片段,序列拼接得到完整RdRp编码基因序列,利用生物学软件进行同源性和种系发生分析,并对RdRp保守功能序列和位点进行预测分析.结果中国狂犬病毒aG和CTN181株的L蛋白分别由6387和6384个核苷酸编码的2128和2127个氨基酸组成,前者比后者在起始部位多编码一个Met.结构功能分析发现在L蛋白上存在若干可能对RNA合成、mRNA聚腺苷酸化和磷蛋白磷酸化以及甲基转移酶的功能起决定作用序列;种系发生树显示中国疫苗株aG相对独立于WHO推荐使用的其他疫苗株.结论完整L基因结构揭示,对于全面了解中国狂犬病毒分子特征,发现新的功能位点,开发以RdRp为靶标的新型抗病毒药物研究以及弹状病毒科种系发生分析提供了有力的工具.
목적 측정중국광견병독인용역묘주aG화감독주CTN181적RdRp편마(L)기인서렬병초보연구기결구화공능특점.방법 RT-PCR법획득약간교차중첩소편단,서렬병접득도완정RdRp편마기인서렬,이용생물학연건진행동원성화충계발생분석,병대RdRp보수공능서렬화위점진행예측분석.결과 중국광견병독aG화CTN181주적L단백분별유6387화6384개핵감산편마적2128화2127개안기산조성,전자비후자재기시부위다편마일개Met.결구공능분석발현재L단백상존재약간가능대RNA합성、mRNA취선감산화화린단백린산화이급갑기전이매적공능기결정작용서렬;충계발생수현시중국역묘주aG상대독립우WHO추천사용적기타역묘주.결론 완정L기인결구게시,대우전면료해중국광견병독분자특정,발현신적공능위점,개발이RdRp위파표적신형항병독약물연구이급탄상병독과충계발생분석제공료유력적공구.
Objective Characterization of RdRp gene (L) of rabies virus aG and CTN181 strain in China. Methods Overlapped fragments were amplified and assembled. Then characterization and phylogenetic analyses as well as prediction of functional regions were performed using biologic software. Results L gene ofCTN181 and aG strains were composed with 6387nt and 6384nt respectively and there were two repeated ATG in the start of L gene in CTN181 strain. In addition, some mutations and new functional regions were discovered and presumed to be crucial to the role of these regions in replication of rabies virus. Analyses of L gene provide new insight into the functional regions, and phylogenetic analyses showed that human vaccine strain aG were independent to others recommended by WHO. Conclusion s The characterization of complete L genes will be helpful for research on new functional regions, antiviral drugs targeted at RdRp and phylogenetic analyses.