CAJ | 학술논문

目的建立一种C57BL/6小鼠前列腺癌原位细胞移植动物模型.方法 C57BL/6小鼠30只,用微量注射器分别将0.5×106个RM-1细胞注射入前列腺左、右背侧叶包膜下.每3 d随机处死5只小鼠,动态观察小鼠前列腺癌局部生长、盆腔淋巴结转移和器官转移;术后15 d处死小鼠后,剩余5只常规饲养直至死亡,观察小鼠的平均荷瘤生存期.结果 C57BL/6小鼠前列腺原位移植RM-1细胞后第3、6、9、12、15天前列腺体积组间差异有统计学意义(P＜0.01);术后第12天开始,出现明显尿潴留、双侧或单侧输尿管扩张、肾脏体积增大、肾盂扩张;组织学观察发现术后前列腺癌细胞逐渐取代正常的前列腺组织,术后第12天组可见肿瘤侵犯周围肌肉,术后第15天组可见肿瘤侵犯精囊和膀胱.术后第3、6、9天未见明显盆腔淋巴结转移,第12、15天盆腔淋巴结转移率均为80%.各组均未发现明显的远处器官转移.各组小鼠成瘤率均为100%.小鼠平均荷瘤生存期为(15.60±0.89)d.结论虽然未见明确的器官转移情况,但该模型能够较好的模拟人类前列腺癌的发生、发展、局部侵袭及淋巴转移的过程,是一种较理想的前列腺癌动物模型.
목적 건립일충C57BL/6소서전렬선암원위세포이식동물모형.방법 C57BL/6소서30지,용미량주사기분별장0.5×106개RM-1세포주사입전렬선좌、우배측협포막하.매3 d수궤처사5지소서,동태관찰소서전렬선암국부생장、분강림파결전이화기관전이;술후15 d처사소서후,잉여5지상규사양직지사망,관찰소서적평균하류생존기.결과 C57BL/6소서전렬선원위이식RM-1세포후제3、6、9、12、15천전렬선체적조간차이유통계학의의(P＜0.01);술후제12천개시,출현명현뇨저류、쌍측혹단측수뇨관확장、신장체적증대、신우확장;조직학관찰발현술후전렬선암세포축점취대정상적전렬선조직,술후제12천조가견종류침범주위기육,술후제15천조가견종류침범정낭화방광.술후제3、6、9천미견명현분강림파결전이,제12、15천분강림파결전이솔균위80%.각조균미발현명현적원처기관전이.각조소서성류솔균위100%.소서평균하류생존기위(15.60±0.89)d.결론 수연미견명학적기관전이정황,단해모형능구교호적모의인류전렬선암적발생、발전、국부침습급림파전이적과정,시일충교이상적전렬선암동물모형.
Objective To investigate the regularity in establishing the prostate cancer orthotopic transplantation model in C57BL/6 mice. Methods RM-1 cells (0.5×106)were injected into the right and left dorsal lateral prostate capsules of 30 C57BL/6 male mice respectively using micro-syringe. Five mice were sacrificed every three days to observe the local growth, the occurrence and development of prostate cancer, and the remaining five mice were fed to death to calculate the average survival period of tumorbearing mice. Results The volume of prostates was significantly different among the five groups. From the 12th day, significant retention of urine, ureterectasia (bilateral or unilateral ), increased kidney volume and pelviectasis were observed. The rate of tumor formation in the five groups which included the 3rd, 6th,9th, 12th d and 15th day was all 100%. HE staining showed that prostate cancer cells gradually replaced the normal prostate tissue. At the 12th day, muscle tissue around prostate was invaded by prostate cancer.In some samples there was metastasis of seminal vesicles and bladder at the 15th day. The rate of metastasis in pelvic lymph nodes at the 12th or 15th day was 80%. The average survival period of tumor-bearing mice was (15.60±0.89) days. Conclusion The prostate cancer orthotopic transplantation model in C57BL/6 mice can better simulate the process of occurrence, development, local invasion and lymph node metastasis of human prostate cancer and be suitably used as a model of prostate cancer research.