CAJ | 학술논문

目的观察免疫抑制剂环孢素A(CsA)对大鼠肾脏成纤维细胞增殖及细胞因子表达的影响以及氟伐他汀的干预作用,探讨氟伐他汀对CsA肾毒性的保护作用.方法体外培养大鼠肾脏成纤维细胞,四甲基偶氮唑盐(MTT)比色法测定CsA及氟伐他汀对细胞增殖的影响.RT-PCR方法检测转化生长因子β1(TGF-β1)、结缔组织生长因子(CTGF)及c-fosmRNA水平.Western印迹检测纤连蛋白(FN)的表达.结果 CsA可明显抑制成纤维细胞增殖,且呈剂量和时间依赖效应(P＜0.05).氟伐他汀与CsA合用后,对细胞增殖有明显的抑制作用(P＜0.01).CsA刺激成纤维细胞TGF-β1、CTGF、c-fos及FN的产生(P＜0.05),氟伐他汀可下调这些改变(P＜0.05).结论氟伐他汀可减轻CsA所致的大鼠肾脏成纤维细胞毒性.
목적 관찰면역억제제배포소A(CsA)대대서신장성섬유세포증식급세포인자표체적영향이급불벌타정적간예작용,탐토불벌타정대CsA신독성적보호작용.방법 체외배양대서신장성섬유세포,사갑기우담서염(MTT)비색법측정CsA급불벌타정대세포증식적영향.RT-PCR방법검측전화생장인자β1(TGF-β1)、결체조직생장인자(CTGF)급c-fosmRNA수평.Western인적검측섬련단백(FN)적표체.결과 CsA가명현억제성섬유세포증식,차정제량화시간의뢰효응(P＜0.05).불벌타정여CsA합용후,대세포증식유명현적억제작용(P＜0.01).CsA자격성섬유세포TGF-β1、CTGF、c-fos급FN적산생(P＜0.05),불벌타정가하조저사개변(P＜0.05).결론 불벌타정가감경CsA소치적대서신장성섬유세포독성.
Objective To investigate the protective effects of fluvastatin(Flu) on rat renal fibroblasts proliferation and cytokines expression induced by cyclosperine A (CsA). Methods The fibroblasts were cultured with CsA or with CsA plus fluvastatin. The cellular proliferation was determined by MTT colorimetry. The mRNA expression of transforming growth factor β1(TGF-[β1), connetive tissue growth factor (CTGF) and c-fos was detected by BT-RCB. The protein level of flbronecfin (FN) was measured by Western blotting. Results CsA inhibited the proliferation of fibroblasts in dose- and time-dependent manner (P＜0.05). Treatment with Flu accelerated the suppression of fibroblasts resulted from CsA (P＜0.O1). CsA stimulated the expression of TGF-β1, CTGF, c-fos and FN compared with control group(P＜0.05), which could be down-regulated by Flu (P＜0.05). Conclusion Fluvastatin may relieve CsA-induced nephrotoxicity in rat fibroblasts.