CAJ | 학술논문

本文对一种新的甜菊糖基转移酶进行了基因克隆和功能分析.获得的基因cDNA全长1419bp,编码473个氨基酸,蛋白质分子量约53.2K Da.与常见的糖基转移酶基因比较,相似性达44%以上,且具有糖基转移酶的保守序列.体外异源表达获得的融合蛋白,具有在花青素类和甜菊醇等糖基受体上转糖基的酶活性.在对一系列不同底物的酶活性进行比较后,推测这种糖基转移酶在体内参与了甜菊糖苷的合成.结果表明,具有广泛的底物活性的类黄酮类糖基转移酶,在甜菊体内不仅对类黄酮转糖基,而且在生成水溶性甜菊糖苷的过程中也扮演重要的角色.
본문대일충신적첨국당기전이매진행료기인극륭화공능분석.획득적기인cDNA전장1419bp,편마473개안기산,단백질분자량약53.2K Da.여상견적당기전이매기인비교,상사성체44%이상,차구유당기전이매적보수서렬.체외이원표체획득적융합단백,구유재화청소류화첨국순등당기수체상전당기적매활성.재대일계렬불동저물적매활성진행비교후,추측저충당기전이매재체내삼여료첨국당감적합성.결과표명,구유엄범적저물활성적류황동류당기전이매,재첨국체내불부대류황동전당기,이차재생성수용성첨국당감적과정중야분연중요적각색.
We repor here the cloning and characterization of a UDP-glucose flavonoid glucosyltransferase(srUFGT) in Stevia rebaudiana. The isolated cDNA was 1419bp in length encoding 473 deduced amino acidswith a predicted molecular mass of 53.2 kDa. The products of in vitro translation from an expression vectorhad anthocyanidins and steviol glucosyltransferase activity. Comparison of the activity of the recombinant UDP-glucosyltransferase toward a range of acceptor substrates suggests that it may participate in the synthesis of ste-viol glycosides. The results support the hypothesis that the flavonoid glucosyltransferases, which have a broadsubstrate specificity,may be not only involved in flavonoid glucosylation but also play a role in producing thewater-soluble steviol-glycosides in S. rebaudiana.