生物技术通报
生物技術通報
생물기술통보
BIOTECHNOLOGY BULLETIN
2010年
3期
119-123
,共5页
番茄%AtNHX1基因%农杆菌介导法%NaHCO_3胁迫
番茄%AtNHX1基因%農桿菌介導法%NaHCO_3脅迫
번가%AtNHX1기인%농간균개도법%NaHCO_3협박
Tomato%AtNHX1 gene%Agrobacterium-mediated method%NaHCO_3 stress
构建AtNHX1基因植物表达载体,通过农杆菌介导法将其转入番茄.探讨了外植体类型、农杆菌菌株和不同筛选标记对芽诱导分化的影响.对抗性植株进行PCR检测,获得15株转基因植株.对转基因番茄T_1代进行80 mmol/L NaHCO_3胁迫处理,转基因植株的相对生长量高于对照植株,显示AtNHX1基因的导入提高了番茄对碱性盐的耐受性.
構建AtNHX1基因植物錶達載體,通過農桿菌介導法將其轉入番茄.探討瞭外植體類型、農桿菌菌株和不同篩選標記對芽誘導分化的影響.對抗性植株進行PCR檢測,穫得15株轉基因植株.對轉基因番茄T_1代進行80 mmol/L NaHCO_3脅迫處理,轉基因植株的相對生長量高于對照植株,顯示AtNHX1基因的導入提高瞭番茄對堿性鹽的耐受性.
구건AtNHX1기인식물표체재체,통과농간균개도법장기전입번가.탐토료외식체류형、농간균균주화불동사선표기대아유도분화적영향.대항성식주진행PCR검측,획득15주전기인식주.대전기인번가T_1대진행80 mmol/L NaHCO_3협박처리,전기인식주적상대생장량고우대조식주,현시AtNHX1기인적도입제고료번가대감성염적내수성.
AtNHX1 gene was successfully transferred into tomato by Agrobacterium-mediated transformation.Several factors that affect the frequency of shoot differentiation such as types of explants,Agrobacterium strains,vectors with different selectable markers were studied.The resistant plants were confirmed by PCR assay,15 transgenic plants were obtained.Relative growth was estimated when they were cultured under 80 mmol/L NaHCO_3 stress.The results indicated that the relative growth of the transgenic plants was higher compared with the control plant.Therefore,the transgenic AtNHX1 tomato enhanced the resistance to NaHCO_3stress.
