中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2010年
3期
573-576
,共4页
张增方%黄世光%黄绮凌%谢敏%邓国珍
張增方%黃世光%黃綺凌%謝敏%鄧國珍
장증방%황세광%황기릉%사민%산국진
牙周炎%白细胞介素-4%模型,动物
牙週炎%白細胞介素-4%模型,動物
아주염%백세포개소-4%모형,동물
Periodontitis%Interleukin-4%Models,animal
目的:通过对实验性牙周炎小鼠接种低剂量旋毛虫(T.spiralis)介导Ⅱ型辅助性 T淋巴细胞(Th2)反应,探讨白细胞介素-4(IL-4)在牙周炎进程中的作用.方法:清洁级12周龄雄性昆明小鼠27只,随机分为3组:(1)正常对照组;(2)实验性牙周炎组:参照Kimura等的方法复制实验性牙周炎模型;(3)旋毛虫感染合并牙周炎组(periodontitis+T. spiralis-infected group,P+T.spiralis):复制小鼠牙周炎模型,同时给予低剂量旋毛虫感染.正常对照组及各实验组小鼠分别于术后第1、3和5周末分批处死,各时点每组处死小鼠数为3只.处死小鼠前测量牙周探诊深度,制作牙体牙周联合切片观察牙周的组织学改变,并采用ELISA法检测牙周炎小鼠外周血IL-4的水平.结果:(1)P+T. spiralis组IL-4的含量明显高于实验性牙周炎组(P<0.01);(2)各时点P+T.spiralis组牙周探诊深度明显低于实验性牙周炎组(P<0.01);(3)P+T. spiralis组牙周组织的炎症及破坏程度明显减轻.结论:旋毛虫感染可升高小鼠血清IL-4水平,抑制牙周炎的发生,提示在牙周炎治疗中提高血清IL-4水平可能具有治疗价值.
目的:通過對實驗性牙週炎小鼠接種低劑量鏇毛蟲(T.spiralis)介導Ⅱ型輔助性 T淋巴細胞(Th2)反應,探討白細胞介素-4(IL-4)在牙週炎進程中的作用.方法:清潔級12週齡雄性昆明小鼠27隻,隨機分為3組:(1)正常對照組;(2)實驗性牙週炎組:參照Kimura等的方法複製實驗性牙週炎模型;(3)鏇毛蟲感染閤併牙週炎組(periodontitis+T. spiralis-infected group,P+T.spiralis):複製小鼠牙週炎模型,同時給予低劑量鏇毛蟲感染.正常對照組及各實驗組小鼠分彆于術後第1、3和5週末分批處死,各時點每組處死小鼠數為3隻.處死小鼠前測量牙週探診深度,製作牙體牙週聯閤切片觀察牙週的組織學改變,併採用ELISA法檢測牙週炎小鼠外週血IL-4的水平.結果:(1)P+T. spiralis組IL-4的含量明顯高于實驗性牙週炎組(P<0.01);(2)各時點P+T.spiralis組牙週探診深度明顯低于實驗性牙週炎組(P<0.01);(3)P+T. spiralis組牙週組織的炎癥及破壞程度明顯減輕.結論:鏇毛蟲感染可升高小鼠血清IL-4水平,抑製牙週炎的髮生,提示在牙週炎治療中提高血清IL-4水平可能具有治療價值.
목적:통과대실험성아주염소서접충저제량선모충(T.spiralis)개도Ⅱ형보조성 T림파세포(Th2)반응,탐토백세포개소-4(IL-4)재아주염진정중적작용.방법:청길급12주령웅성곤명소서27지,수궤분위3조:(1)정상대조조;(2)실험성아주염조:삼조Kimura등적방법복제실험성아주염모형;(3)선모충감염합병아주염조(periodontitis+T. spiralis-infected group,P+T.spiralis):복제소서아주염모형,동시급여저제량선모충감염.정상대조조급각실험조소서분별우술후제1、3화5주말분비처사,각시점매조처사소서수위3지.처사소서전측량아주탐진심도,제작아체아주연합절편관찰아주적조직학개변,병채용ELISA법검측아주염소서외주혈IL-4적수평.결과:(1)P+T. spiralis조IL-4적함량명현고우실험성아주염조(P<0.01);(2)각시점P+T.spiralis조아주탐진심도명현저우실험성아주염조(P<0.01);(3)P+T. spiralis조아주조직적염증급파배정도명현감경.결론:선모충감염가승고소서혈청IL-4수평,억제아주염적발생,제시재아주염치료중제고혈청IL-4수평가능구유치료개치.
AIM: The purpose of this study was to investigate the role of interleukin-4 (IL-4) in the development of periodontitis in mice by low dosage of Trichinella spiralis (T. spiralis) infection.METHODS: Twenty-seven male Kunming mice in specific pathogen free grade were randomly divided into three groups: (1) the normal control group;(2) the experimental periodontitis group, which was produced by ligature of braided silk around the first maxillary molar, and was inoculated with putative periodontopathic bacteria;(3) the periodontitis with T. spiralis infection group. The mice were sacrificed at the end of 1, 3 and 5 weeks. The probing depth (PD) was measured before the mice were euthanized. The histological change of periodontal tissues was observed under the microscope after the samples were stained with hematoxylin and eosin. Furthermore, the serum level of IL-4 was determined by ELISA. RESULTS: (1) The serum level of IL-4 in T. spiralis-infected group was significantly higher than that in experimental periodontitis group (P<0.01). (2) The PD in T. spiralis-infected group was significantly lower than that in experimental periodontitis group (P<0.01). (3) Only a mild inflammatory response was observed in T. spiralis-infected animals. CONCLUSION: T. spiralis infection upregulates IL-4 expression and attenuates periodontitis in mice.