CAJ | 학술논문

目的探讨诱导型一氧化氮合酶(iNOS)与Cajal间质细胞(ICC)在术后肠梗阻大鼠小肠中的表达及意义.方法选取健康成年Wistar大鼠30只,按照随机数字表法将其分为对照组和肠梗阻组,每组15只,检测两组大鼠的胃肠道传输速率;应用免疫组织化学染色法对两组大鼠小肠iNOS和c-kit的分布和表达进行观察,应用实时荧光定量PCR检测两组大鼠小肠c-kit和iNOS mRNA的表达.组间比较采用t检验.结果肠梗阻组有2只大鼠因麻醉药物过量或低温死亡,最终进入结果分析的肠梗阻组大鼠为13只.肠梗阻组大鼠胃肠道传输速率为42％±14％,低于对照组大鼠的64％±13％(t=6.56,P＜0.05).免疫组织化学染色法结果显示,肠梗阻组大鼠小肠c-kit阳性细胞数为8.9±2.9,明显少于对照组大鼠的14.4 ±5.0(t =3.97,P＜0.05);肠梗阻组大鼠小肠iNOS阳性细胞表达明显增加,达到13.6±4.9,而对照组大鼠小肠几乎无iNOS阳性细胞;实时荧光定量PCR检测结果表明,术后肠梗阻组大鼠小肠c-kit和iNOS mRNA相对表达量分别为1.6±0.8和2.2±1.2,而对照组分别为2.6±1.1和0.5 ±0.5,两组比较,差异有统计学意义(t =2.86,4.61,P＜0.05).结论大鼠术后肠梗阻发生过程中存在ICC分布减少而iNOS表达明显增加的现象,两者的变化可能在术后肠梗阻发生机制中发挥了重要作用.
목적 탐토유도형일양화담합매(iNOS)여Cajal간질세포(ICC)재술후장경조대서소장중적표체급의의.방법 선취건강성년Wistar대서30지,안조수궤수자표법장기분위대조조화장경조조,매조15지,검측량조대서적위장도전수속솔;응용면역조직화학염색법대량조대서소장iNOS화c-kit적분포화표체진행관찰,응용실시형광정량PCR검측량조대서소장c-kit화iNOS mRNA적표체.조간비교채용t검험.결과 장경조조유2지대서인마취약물과량혹저온사망,최종진입결과분석적장경조조대서위13지.장경조조대서위장도전수속솔위42％±14％,저우대조조대서적64％±13％(t=6.56,P＜0.05).면역조직화학염색법결과현시,장경조조대서소장c-kit양성세포수위8.9±2.9,명현소우대조조대서적14.4 ±5.0(t =3.97,P＜0.05);장경조조대서소장iNOS양성세포표체명현증가,체도13.6±4.9,이대조조대서소장궤호무iNOS양성세포;실시형광정량PCR검측결과표명,술후장경조조대서소장c-kit화iNOS mRNA상대표체량분별위1.6±0.8화2.2±1.2,이대조조분별위2.6±1.1화0.5 ±0.5,량조비교,차이유통계학의의(t =2.86,4.61,P＜0.05).결론 대서술후장경조발생과정중존재ICC분포감소이iNOS표체명현증가적현상,량자적변화가능재술후장경조발생궤제중발휘료중요작용.
Objective To investigate the change and significance of inducible nitric oxide synthase (iNOS) and intersititial cells of Cajal (ICC) in the small intestine of rats with postoperative ileus (POI).Methods Thirty healthy adult Wistar rats were randomly divided into the control group ( 15 rats) and POI group ( 15 rats) according to the random number table.The gastrointestinal transit rates of the 2 groups were detected.The distributions and expressions of iNOS and c-kit in the small intestines of the rats in the 2 groups were detected by immunohistochemical staining,and the mRNA expressions of c-kit and iNOS were examined by quantitative real-time polymerase chain reaction (qRT-PCR).All data were analyzed by the t test.Results Two rats in the POI group died of excessive dosage of anaesthesia or hypothermia,and the remaining 13 rats in the POI group were included in the analysis.The gastrointestinal transit rate was 42％ ± 14％ in the POI group,which was significantly lower than 64％ ± 13％ in the control group ( t =6.56,P ＜ 0.05 ).The results of immunohistochemical staining showed that the number of cells with c-kit positive staining was 8.9 ± 2.9,which was significantly smaller than 14.4 ± 5.0 in the control group ( t =3.97,P ＜ 0.05 ).The number of cells with iNOS immune-positive reaction increased to 13.6 ±4.9,while there was nearly no cells with iNOS immune-positive reaction in the control group.The results of qRT-PCR showed that the relative mRNA expressions of c-kit and iNOS were 1.6 ± 0.8 and 2.2 ± 1.2 in the POI group,and 2.6 ± 1.1,0.5 ± 0.5 in the control group.There were significant differences on the mRNA expressions of c-kit and iNOS between the POI group and the control group ( t =2.86,4.61,P ＜ 0.05).Conclusions ICC reduction and iNOS increase are observed in rats with POI.The change of the 2 factors may plav an important role in the mechanisms of POI.