CAJ | 학술논문

目的分析雏鸡形觉剥夺性近视眼及形觉剥夺性近视恢复眼后极部巩膜中基质金属蛋白酶2(matrix metallopmteinaae 2,MMP-2)、组织金属蛋白酶抑制剂2(tissue inhibitor of matrix metallopro-teinase 2,TIMP-2)及视黄酸受体-β(retinoic acid receptor-β,RARβ)的水平及其相关性.方法选用新孵出家鸡75只,半透明眼罩遮盖的方法对左眼进行形觉剥夺,分为形觉剥夺组,遮盖14d;形觉剥夺恢复组,遮盖11d后,去遮盖3d.右眼为对照眼.取直径8mm的后极部眼组织块,分离出巩膜纤维层和软骨层.RT-PCR检测MMP-2、TIMP-2及RARβ mRNA的相对含量,并将MMP-2、TIMP-2 mRNA水平分别与RARβ的mRNA水平作线性相关分析.结果形觉剥夺14d后,纤维层中MMP-2的表达增强(P<0.01),TIMP-2的表达减弱(P<0.01);去剥夺3d后,MMP-2的表达减弱(P<0.01),而TIMP-2的表达恢复到接近对照眼的水平(P>0.05).软骨层表达没有明显变化.形觉剥夺14d后,RARβ的表达均增强,但纤维层中的表达更为强烈(P<0.01).去剥夺3d后,RARβ的表达均明显下降(P<0.01).MMP-2与RARβmRNA水平呈正相关(P<0.05,r=0.944);TIMP-2与RARβmRNA水平呈负相关(P<0.05,r=-0.863).结论雏鸡形觉剥夺性近视眼及形觉剥夺性近视恢复眼后极部巩膜纤维层中MMP-2、TIMP-2及RARβ的水平均发生明显变化,并有相关性.RARβ参与了巩膜纤维层细胞外基质的降解过程.
목적 분석추계형각박탈성근시안급형각박탈성근시회복안후겁부공막중기질금속단백매2(matrix metallopmteinaae 2,MMP-2)、조직금속단백매억제제2(tissue inhibitor of matrix metallopro-teinase 2,TIMP-2)급시황산수체-β(retinoic acid receptor-β,RARβ)적수평급기상관성.방법 선용신부출가계75지,반투명안조차개적방법대좌안진행형각박탈,분위형각박탈조,차개14d;형각박탈회복조,차개11d후,거차개3d.우안위대조안.취직경8mm적후겁부안조직괴,분리출공막섬유층화연골층.RT-PCR검측MMP-2、TIMP-2급RARβ mRNA적상대함량,병장MMP-2、TIMP-2 mRNA수평분별여RARβ적mRNA수평작선성상관분석.결과 형각박탈14d후,섬유층중MMP-2적표체증강(P<0.01),TIMP-2적표체감약(P<0.01);거박탈3d후,MMP-2적표체감약(P<0.01),이TIMP-2적표체회복도접근대조안적수평(P>0.05).연골층표체몰유명현변화.형각박탈14d후,RARβ적표체균증강,단섬유층중적표체경위강렬(P<0.01).거박탈3d후,RARβ적표체균명현하강(P<0.01).MMP-2여RARβmRNA수평정정상관(P<0.05,r=0.944);TIMP-2여RARβmRNA수평정부상관(P<0.05,r=-0.863).결론 추계형각박탈성근시안급형각박탈성근시회복안후겁부공막섬유층중MMP-2、TIMP-2급RARβ적수평균발생명현변화,병유상관성.RARβ삼여료공막섬유층세포외기질적강해과정.
Objective To analyze the level and relativity of matrix metalloproteinase 2 (MMP-2),tissue inhibitor of matrix metalloproteinase 2 (TIMP-2) and retinoic acid receptor-β (RARβ) of posterior sclera in chicken form-deprivation myopic eyes and form-deprivation myopic recovery eyes.Methods Seven-ty-five newly incubated carnivorous chickens were selected.Their left eyes were covered with semitransparent membrane to deprive form perception, and were divided into 2 trial groups: form-deprived group: covering for 14 days, and recovery group: covering for 11 days followed by uncovering for 3 days.Posterior ocular tissue of 8mm in diameter was taken.Scleral fiber layer and cartilage layer were isolated.RT-PCR was used to detect the level of MMP-2, TIMP-2 and RARβ.The relativity between the mRNA of MMP-2, TIMP-2 and the mRNA of RARβ were analyzed respectively.Results There was strong expression of MMP-2 and TIMP-2 in fibrous sclera, while there was very weak expression in cartilage sclera.There were no obvious changes in the cartilage sclera in form-deprivation and recovery from form-deprivation.While in the fibrous sclera, MMP-2 was in-creased (P<0.01), TIMP-2 was decreased (P<0.01) at 14 days of form-deprivation.At 3 days recovery from form-deprivation, MMP-2 was decreased (P<0.01), TIMP-2 was increased (P <0.05).At 14 days of form-deprivation, RAPβ increased in the sclera.It increased more obvious in the fibrous sclera (P<0.01).At 3 days recovery from form-deprivation, RARβ decreased.The relative content of mRNA of MMP-2 was posi-tive correlation with that of RARβ (P<0.05, r=0.944).The relative content of mRNA of TIMP-2 was nega-tive correlation with that of RARβ (P<0.05, r=-0.863).Conclusions During form-deprivation and form-de-privation recovery, MMP-2, TIMP-2 and RARβ contents in posterior fibrous sclera change significantly.RARβ takes part in the degeneration process of fibrous scleral extra cellular matrix.