中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2010年
4期
309-312
,共4页
李珍%章功良%王斌生%张瑾%孔德虎%王烈成
李珍%章功良%王斌生%張瑾%孔德虎%王烈成
리진%장공량%왕빈생%장근%공덕호%왕렬성
双酚A%长时程增强%脑发育%学习记忆
雙酚A%長時程增彊%腦髮育%學習記憶
쌍분A%장시정증강%뇌발육%학습기억
Bisphenol A%Long-term potentiation%Cerebral development%Learning and memory
目的 研究围生期双酚A暴露对雄性子代大鼠探索行为及学习记忆能力的影响.方法 自妊娠11 d开始直至产后7 d,一组母鼠每I:1皮下注射10μg/kg双酚A(BPA),另一组母鼠注射等量的BPA的溶剂.在大鼠出生后21 d进行开场实验、水迷宫实验,同时制作海马脑片,用电生理学方法 检测BPA暴露对海马CA1区LTP诱导的影响.结果 BPA模型组大鼠穿越格子数[(70.35±13.56)次]明显高于对照组[(29.32±14.12)次,P＜0.05];BPA模型组大鼠站立次数、理毛次数[分别为(38.52±6.52)次,(6.26±2.78)次]均较对照组大鼠显著增加[分别为(10.35±8.38)次,(2.67±1.46)次,P＜0.05].Morris水迷宫实验中,BPA模型组大鼠寻找平台的潜伏期[(55.22±5.78)s]较对照组显著延长[(21.22±2.65)s,P＜0.05];高频刺激后对照组海马CAI区可诱导稳定的LTP[(162.13±10.12)%],BPA模型组大鼠海马CA1区LTP诱导障碍[(101.05±7.58)%,P＜0.05].结论 围生期BPA暴露损伤了雄性子代大鼠的学习记忆能力,其机制可能和突触前功能受损有关.
目的 研究圍生期雙酚A暴露對雄性子代大鼠探索行為及學習記憶能力的影響.方法 自妊娠11 d開始直至產後7 d,一組母鼠每I:1皮下註射10μg/kg雙酚A(BPA),另一組母鼠註射等量的BPA的溶劑.在大鼠齣生後21 d進行開場實驗、水迷宮實驗,同時製作海馬腦片,用電生理學方法 檢測BPA暴露對海馬CA1區LTP誘導的影響.結果 BPA模型組大鼠穿越格子數[(70.35±13.56)次]明顯高于對照組[(29.32±14.12)次,P＜0.05];BPA模型組大鼠站立次數、理毛次數[分彆為(38.52±6.52)次,(6.26±2.78)次]均較對照組大鼠顯著增加[分彆為(10.35±8.38)次,(2.67±1.46)次,P＜0.05].Morris水迷宮實驗中,BPA模型組大鼠尋找平檯的潛伏期[(55.22±5.78)s]較對照組顯著延長[(21.22±2.65)s,P＜0.05];高頻刺激後對照組海馬CAI區可誘導穩定的LTP[(162.13±10.12)%],BPA模型組大鼠海馬CA1區LTP誘導障礙[(101.05±7.58)%,P＜0.05].結論 圍生期BPA暴露損傷瞭雄性子代大鼠的學習記憶能力,其機製可能和突觸前功能受損有關.
목적 연구위생기쌍분A폭로대웅성자대대서탐색행위급학습기억능력적영향.방법 자임신11 d개시직지산후7 d,일조모서매I:1피하주사10μg/kg쌍분A(BPA),령일조모서주사등량적BPA적용제.재대서출생후21 d진행개장실험、수미궁실험,동시제작해마뇌편,용전생이학방법 검측BPA폭로대해마CA1구LTP유도적영향.결과 BPA모형조대서천월격자수[(70.35±13.56)차]명현고우대조조[(29.32±14.12)차,P＜0.05];BPA모형조대서참립차수、리모차수[분별위(38.52±6.52)차,(6.26±2.78)차]균교대조조대서현저증가[분별위(10.35±8.38)차,(2.67±1.46)차,P＜0.05].Morris수미궁실험중,BPA모형조대서심조평태적잠복기[(55.22±5.78)s]교대조조현저연장[(21.22±2.65)s,P＜0.05];고빈자격후대조조해마CAI구가유도은정적LTP[(162.13±10.12)%],BPA모형조대서해마CA1구LTP유도장애[(101.05±7.58)%,P＜0.05].결론 위생기BPA폭로손상료웅성자대대서적학습기억능력,기궤제가능화돌촉전공능수손유관.
Objective To investigate the effects of bisphenol A(BPA)exposure to perinatal on learning and memory behaviour in F1 generation male rats.Methods From the 11th day of gestation to the 7th day of field test was used to detect the locomotion activity,the Morris water maze was employed to measure learning and memory ability and long-term potentiation(LTP)were recorded to detect the synaptic plasticity in hippocampus CA1 with extracellular recording.Results The crossing number of BPA group(70.35±13.56)was much more than control group((29.32±14.12),P＜0.05).The number of standing up and ornament of BPA group((38.52±6.52),(6.26±2.78))were both higher than control group((10.35±8.38),(2.67±1.46),P＜0.05).BPA rats had longer escape latencies to find platform((55.22±5.78)s)than control rats((21.22±2.65)s,P＜0.05).Conditional protocol with high-frequency stimulation evoked a stable LTP in hippocampus CA1 region in control rats((162.13±10.12)%),however the LTP could not be induced with the same conditional protocol in BPA-exposed rats((101.05±7.58)%,P＜0.05).Conclusion Perinatal BPA exposure impaires learning and memory ability in F1 generation male rats related to presynaptic dysfunction.
