中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2009年
5期
321-323
,共3页
杜志勇%魏翠凤%田锐%江建新%王敏%秦仁义
杜誌勇%魏翠鳳%田銳%江建新%王敏%秦仁義
두지용%위취봉%전예%강건신%왕민%진인의
胰腺肿瘤%干细胞%肿瘤细胞系%抗药性%肿瘤%标志物
胰腺腫瘤%榦細胞%腫瘤細胞繫%抗藥性%腫瘤%標誌物
이선종류%간세포%종류세포계%항약성%종류%표지물
Pancreatic neoplasms%Stem cells%Tumor cell line%Drug resistance%neoplasm%Marker
目的 观察放化疗抵抗的胰腺癌细胞的Bcl-2、survivin及干细胞标志物Oct-4、ABCG2蛋白表达的变化,探讨这部分肿瘤细胞耐受放化疗的原因.方法 采用同步放化疗干预人胰腺癌细胞系SW1990、BxPC3、pc3、jr305,获得放化疗抵抗的胰腺癌细胞,以不行放化疗处理的细胞作为对照.采用Western blotting检测它们的Bcl-2、survivin、Oct-4和ABCG2蛋白的表达变化.结果 放化疗抵抗的SW1990、BxPC3、pc3、jt305胰腺癌细胞Bcl-2蛋白表达量分别为0.7955±0.0326、0.5718±0.0212、0.6137±0.0382和0.8733±0.0461;survivin蛋白表达量分别为0.8207±0.0490、0.6973±0.021 1、0.7967±0.0346和0.8013±0.0398;Oct-4蛋白表达量分别为0.8728±0.0177、0.7861±0.0139、0.4794±0.0932和0.4216±0.1043;ABCG2蛋白表达量分别为0.7810±0.1370、0.4957±0.1126、0.6102±0.1358和0.4670±0.1274.对照组相应细胞系的4种蛋白表达量分别为0.4723±0.018、0.2954±0.0103、0.3587±0.0201和0.2718±0.0136;0.4717±0.0274、0.3587±0.0113、0.3891±0.0147和0.3326±0.0124;0.6053±0.0142、0.4236±0.0086、0.2385±0.0671和0.1985±0.0582;0.3156±0.0582、0.2360±0.0423、0.2813±0.0512和0.1808±0.0370.放化疗后胰腺癌细胞的4种蛋白表达量均显著增加(P＜0.05).结论 放化疗抵抗的胰腺癌细胞中可能富含肿瘤干细胞.
目的 觀察放化療牴抗的胰腺癌細胞的Bcl-2、survivin及榦細胞標誌物Oct-4、ABCG2蛋白錶達的變化,探討這部分腫瘤細胞耐受放化療的原因.方法 採用同步放化療榦預人胰腺癌細胞繫SW1990、BxPC3、pc3、jr305,穫得放化療牴抗的胰腺癌細胞,以不行放化療處理的細胞作為對照.採用Western blotting檢測它們的Bcl-2、survivin、Oct-4和ABCG2蛋白的錶達變化.結果 放化療牴抗的SW1990、BxPC3、pc3、jt305胰腺癌細胞Bcl-2蛋白錶達量分彆為0.7955±0.0326、0.5718±0.0212、0.6137±0.0382和0.8733±0.0461;survivin蛋白錶達量分彆為0.8207±0.0490、0.6973±0.021 1、0.7967±0.0346和0.8013±0.0398;Oct-4蛋白錶達量分彆為0.8728±0.0177、0.7861±0.0139、0.4794±0.0932和0.4216±0.1043;ABCG2蛋白錶達量分彆為0.7810±0.1370、0.4957±0.1126、0.6102±0.1358和0.4670±0.1274.對照組相應細胞繫的4種蛋白錶達量分彆為0.4723±0.018、0.2954±0.0103、0.3587±0.0201和0.2718±0.0136;0.4717±0.0274、0.3587±0.0113、0.3891±0.0147和0.3326±0.0124;0.6053±0.0142、0.4236±0.0086、0.2385±0.0671和0.1985±0.0582;0.3156±0.0582、0.2360±0.0423、0.2813±0.0512和0.1808±0.0370.放化療後胰腺癌細胞的4種蛋白錶達量均顯著增加(P＜0.05).結論 放化療牴抗的胰腺癌細胞中可能富含腫瘤榦細胞.
목적 관찰방화료저항적이선암세포적Bcl-2、survivin급간세포표지물Oct-4、ABCG2단백표체적변화,탐토저부분종류세포내수방화료적원인.방법 채용동보방화료간예인이선암세포계SW1990、BxPC3、pc3、jr305,획득방화료저항적이선암세포,이불행방화료처리적세포작위대조.채용Western blotting검측타문적Bcl-2、survivin、Oct-4화ABCG2단백적표체변화.결과 방화료저항적SW1990、BxPC3、pc3、jt305이선암세포Bcl-2단백표체량분별위0.7955±0.0326、0.5718±0.0212、0.6137±0.0382화0.8733±0.0461;survivin단백표체량분별위0.8207±0.0490、0.6973±0.021 1、0.7967±0.0346화0.8013±0.0398;Oct-4단백표체량분별위0.8728±0.0177、0.7861±0.0139、0.4794±0.0932화0.4216±0.1043;ABCG2단백표체량분별위0.7810±0.1370、0.4957±0.1126、0.6102±0.1358화0.4670±0.1274.대조조상응세포계적4충단백표체량분별위0.4723±0.018、0.2954±0.0103、0.3587±0.0201화0.2718±0.0136;0.4717±0.0274、0.3587±0.0113、0.3891±0.0147화0.3326±0.0124;0.6053±0.0142、0.4236±0.0086、0.2385±0.0671화0.1985±0.0582;0.3156±0.0582、0.2360±0.0423、0.2813±0.0512화0.1808±0.0370.방화료후이선암세포적4충단백표체량균현저증가(P＜0.05).결론 방화료저항적이선암세포중가능부함종류간세포.
Objective To investigate the expression of Bcl-2,survivin and pancreatic cancer stem cells markers Oct-4 and ABCG2 in pancreatic cancer cells resistance to chemoradiotherapy.and explore its mechanism.Methods Concurrent ehemoradiotherapy was used to obtain pancreatic cancer cells resistant to chemoradiotherapy,the pancreatic cancer cells without chemoradiotherapy treatment were used as control.Western-blot was applied to detect the expression of Bcl-2,survivin,Oct-4,ABCG2.Results The expression of Bcl-2 was 0.7955±0.0326,0.5718±0.0212,0.6137±0.0382 and 0.8733±0.0461,respectively;the expression of survivin protein was 0.8207±0.0490,0.6973±0.0211,0.7967±0.0346 and 0.8013±0.0398,respectively;the expression of Oct-4 protein was 0.8728±0.0177,0.7861±0.0139,0.4794±0.0932 and 0.4216±0.1043,respectively;the expression of ABCG2 protein was 0.7810±0.1370,0.4957±0.1126,0.6102±0.1358 and 0.4670±0.1274,respectively.in resistant pancreatic cancer cells of SW1990,BxPC3,pc3,jf305 cell line.The corresponding values in the control group were 0.4723±0.018,0.2954±0.0103.0.3587±0.0201 and 0.2718±0.0136;0.4717±0.0274,0.3587±0.0113,0.3891±0.0147 and 0.3326±0.0124;0.6053±0.0142,0.4236±0.0086.0.2385±0.0671 and 0.1985±0.0582;0.3156±0.0582.0.2360±0.0423,0.2813±0.0512 and 0.1808±0.a0370.The expression of all the four proteins significantly increased after ehemoradiotherapy(P＜0.05).Conclusions Pancreatic cancer cells resistant to chemoradiotherapy may contain cancer stem cells.
