CAJ | 학술논문

目的冰冻切片染色法观察重组狂犬病病毒对乳鼠脑的感染和定位.方法将本实验室构建并拯救成功的人用狂犬病疫苗株重组病毒(CTN-GFP)脑内接种1日龄乳鼠和4周龄成年鼠,待感染乳鼠发病后至濒死期,通过心内灌流方法固定并制作脑组织冰冻切片,观察重组病毒在感染鼠的大脑海马组织中的绿色荧光蛋白的表达及分布.结果 DAPI将所制作的冰冻切片中脑组织内细胞的胞核染成蓝色,在共聚焦显微镜下观察到受感染乳鼠的脑组织海马回位置有大量绿色荧光蛋白的表达.结论通过制作脑组织冰冻切片的方法可以清楚的观察到病毒的感染及其在海马回中的位置,同时为追踪病毒在体内的移行过程提供了一种新方法.
목적 빙동절편염색법관찰중조광견병병독대유서뇌적감염화정위.방법 장본실험실구건병증구성공적인용광견병역묘주중조병독(CTN-GFP)뇌내접충1일령유서화4주령성년서,대감염유서발병후지빈사기,통과심내관류방법고정병제작뇌조직빙동절편,관찰중조병독재감염서적대뇌해마조직중적록색형광단백적표체급분포.결과 DAPI장소제작적빙동절편중뇌조직내세포적포핵염성람색,재공취초현미경하관찰도수감염유서적뇌조직해마회위치유대량록색형광단백적표체.결론 통과제작뇌조직빙동절편적방법가이청초적관찰도병독적감염급기재해마회중적위치,동시위추종병독재체내적이행과정제공료일충신방법.
Objective To observe the existence and location of the recombinant rabies virus in the hippocampus of the sulking mice infected recombinant rabies virus.Method A group of one-day-old sulking mice and 4-week-old mice were challenged with the CTN-GFP strain by intracerebral inoculation,frozen longitudinal transect sections of hippocampus were prepared from the suckling mice in order to observe the expression of the GFP protein and the location of the recombinant rabies virus.Results DAPI was performed to stain the cell nuclei in blue while GFP expression from CTN-GFP infected brain cells was observed under a confocal microscope.Conclusion The location of the rabies virus can be clearly observed by preparing frozen section of certain sites from the brain,and this method also provide a new tool to trace the route of spread of the rabies virus within the animal host.