清华大学学报(英文版)
清華大學學報(英文版)
청화대학학보(영문판)
TSINGHUA SCIENCE AND TECHNOLOGY
2003年
4期
493-497
,共5页
李冬海%阮翔%许强%王可玢%公衍道%匡廷云%张秀芳%赵南明
李鼕海%阮翔%許彊%王可玢%公衍道%劻廷雲%張秀芳%趙南明
리동해%원상%허강%왕가분%공연도%광정운%장수방%조남명
photosystem II (PSII)%chlorophyll fluorescence%pH treatment
The effect of varying pH on the photosystem II (PSII) membrane was studied using absorption and steady-state fluorescence spectroscopy, and using a variable fluorescence technique.pH variations induced significant changes in the chlorophyll states of the PSII membrane, but no effect was seen on the chlorophyll fluorescence parameter F′v/F′m.For acidic pH conditions, protein structures of the PSII membrane were slightly altered, whilst at alkaline pH levels, large changes in the protein structure of the PSII membrane were detected.The results indicate that the microenvironment around Cys in the PSII membrane is very susceptible to alkaline pH conditions, and that in the acid (4≤pH<7) and alkaline (9≥pH>7) regions, pH variation has no effect on the protein structures of the PSII reaction center (RC).
