CAJ | 학술논문

目的:探讨天津地区汉族人群TCF7L2基因rs12255372位点多态性与2型糖尿病(T2DM)发生的相关性.方法:随机抽取352例T2DM患者及176例健康对照者,提取基因组DNA,PCR扩增目的片段后,应用变性高效液相色谱技术(DHPLC)检测该位点基因型,并抽取不同峰型进行DNA测序.分析不同基因型频率的组间差异,Logistic回归分析相关危险因素.结果:TCF7L2基因rs12255372位点的G/T杂合基因型在T2DM组和对照组中的频率分别为18.5%和2.8%,差异有统计学意义(P < 0.01).G/T基因型、糖化血红蛋白、尿素氮、收缩压为2型糖尿病的独立危险因素,高密度脂蛋白为保护因素.T2DM不同并发症患者G/T基因型频率差异无统计学意义(P ＞ 0.05).结论:TCF7L2基因的rs12255372位点G/T基因型携带者发生T2DM危险性增大,该位点可能与天津地区汉族人群T2DM的发病有关,是2型糖尿病发生的危险因素之一.
목적:탐토천진지구한족인군TCF7L2기인rs12255372위점다태성여2형당뇨병(T2DM)발생적상관성.방법:수궤추취352례T2DM환자급176례건강대조자,제취기인조DNA,PCR확증목적편단후,응용변성고효액상색보기술(DHPLC)검측해위점기인형,병추취불동봉형진행DNA측서.분석불동기인형빈솔적조간차이,Logistic회귀분석상관위험인소.결과:TCF7L2기인rs12255372위점적G/T잡합기인형재T2DM조화대조조중적빈솔분별위18.5%화2.8%,차이유통계학의의(P < 0.01).G/T기인형、당화혈홍단백、뇨소담、수축압위2형당뇨병적독립위험인소,고밀도지단백위보호인소.T2DM불동병발증환자G/T기인형빈솔차이무통계학의의(P ＞ 0.05).결론:TCF7L2기인적rs12255372위점G/T기인형휴대자발생T2DM위험성증대,해위점가능여천진지구한족인군T2DM적발병유관,시2형당뇨병발생적위험인소지일.
Objective: To detect the relationship between variant of rsl255372 in transcription factor 7-Like 2(TCF7L2) gene and the type 2 diabetes mellitus (T2DM) in Tianjin Han populations. Methods: Three hundred and fifty-two T2DM patients and 176 healthy controls were randomly selected to extract the genome DNA. Denaturing high performance liquid chromatography was used to detect the PCR amplified fragment. The different peaks were chosen for sequencing. The differences of different genotype frequency between the groups were analysed. The Logistic regression was used to evaluate the risk factors of T2DM. Results: The G/T genotype frequencies of rsl2255372 were 18.5% and 2.8% in T2DM group and healthy control group respectively (P < 0.05). The G/T genotype, glycosylated hemoglobin, urea nitrogen and the systolic blood pressure were the independent risk factors of T2DM, and the high density lipoprotein was the protective factor. There was no significant difference of the G/T genotype frequency in T2DM patients with different complications. Conclusion: This study indicates that G/T genotype in the TCF7L2 gene significantly contributes to T2DM susceptibility in Tianjin Han populations. The G/T genotype is one of the risk factors of the T2DM.