CAJ | 학술논문

采用响应面法优化工艺条件,制备具有较好机械强度的海藻酸盐-壳聚糖-海藻酸盐(ACA)液芯微胶囊.首先,通过Plackett-Burman方法对相关影响因素的效应进行评价,筛选出有显著效应的壳聚糖浓度、壳聚糖溶液pH值和柠檬酸钠溶液pH值3个因素;其次,采用最陡爬坡实验接近具有最好膜强度的中心点区域;最后由中心组合实验和响应面分析确定主要影响因子的最佳条件.在优化工艺条件下验证膜的机械强度,膨胀率-14.62%与优化结果-15.41%接近,优化结果与实际情况较好吻合.在优化条件基础上进行传质性能和细胞生长特性实验,研究结果表明ACA液芯微胶囊比ACA固芯微胶囊具有更好的传质特性,将ACA液芯微胶囊进行细胞包埋,液芯微胶囊较固芯微胶囊具有更高的细胞密度.
채용향응면법우화공예조건,제비구유교호궤계강도적해조산염-각취당-해조산염(ACA)액심미효낭.수선,통과Plackett-Burman방법대상관영향인소적효응진행평개,사선출유현저효응적각취당농도、각취당용액pH치화저몽산납용액pH치3개인소;기차,채용최두파파실험접근구유최호막강도적중심점구역;최후유중심조합실험화향응면분석학정주요영향인자적최가조건.재우화공예조건하험증막적궤계강도,팽창솔-14.62%여우화결과-15.41%접근,우화결과여실제정황교호문합.재우화조건기출상진행전질성능화세포생장특성실험,연구결과표명ACA액심미효낭비ACA고심미효낭구유경호적전질특성,장ACA액심미효낭진행세포포매,액심미효낭교고심미효낭구유경고적세포밀도.
The preparation conditions of alginate-chitosan-alginate (ACA) microcapsules with liquid core and good mechanical strengthwere optimized by response surface method. In the fast optimization step, a Plackett-Burman design was used to evaluate the influence of related factors. The concentration and pH of chitosan solution have a positive effect to the membrane mechanical strength, while the pH of sodium citrate has a negativeeffect. The rest of other preparation conditions has no significant influence on the membrane mechanical strength. The path of steepest ascent was used to approach the optimal region of the optimized conditions subsequently. In the third step, the concentration and pH of chitosan solution and the pH of sodium citratethe were further optimized using central composite designs and response surface analysis. Verification experiment under the optimized conditions presented a S_w(%) value of - 14.62%, which is in line with the theoretical value of - 15.41%. Under the optimized condition, ACA microcapsules with liquid core demonstrate good performance in mass transferring and biocompatibility.