中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2010年
2期
139-141
,共3页
刚亚栋%王俊玲%王平贵%张本忠%王耀玲%李晋
剛亞棟%王俊玲%王平貴%張本忠%王耀玲%李晉
강아동%왕준령%왕평귀%장본충%왕요령%리진
氟化物中毒%精子能动性%精子计数
氟化物中毒%精子能動性%精子計數
불화물중독%정자능동성%정자계수
Fluoride poisoning%Sperm motility%Sperm count
目的 探讨慢性氟中毒对大鼠精子活动度的影响,为氟的生殖毒性研究提供实验依据.方法 健康雄性Wistar大鼠32只,体质量150～180 g,按体质量随机分为4组:生理盐水(对照)组、低、中、高氟组(100、200、300mg·kg~(-1)·d~(-1)NaF),灌胃染毒90 d,每天称体质量.染毒结束后处死大鼠,摘取肝脏、肾脏、睾丸,称质量并计算脏器系数;取附睾游离精子,WLJY-9000型伟力彩色精子质量检测系统测定精子运动参数.结果 染毒第30天低、中氟组大鼠体质量[(235.00±14.56)、(235.44±24.99)g]高于高氟组[(206.00 ±18.16)g,P均<0.05)];第60、90天组大鼠体质量比较差异无统计学意义(F值分别为0.578、1.893,P均>0.05).大鼠肝脏系数、肾脏系数、睾丸系数组间比较差异无统计学意义(F值分别为2.148、0.907、1.801,P均>0.05).精子运动参数,平均路径速度(VAP)高氟组[(25.04 ±4.59)μm/s]较对照组[(20.22 ±3.29)μm/s]升高;直线速度(VSL)低、中、高氟组[(18.82 ±3.19)、(17.84 ±4.54)、(16.46 ±2.63)μm/s]较对照组[(12.48 ±1.73)μm/s]升高;直线性(LIN)低、中、高氟组[(23.84±1.58)%、(24.99±3.37)%、(26.75 ±5.07)%]较对照组[(33.29±4.00)%]降低,摆动性(WOB)中、高氟组[(47.03 ±3.98)%、(49.21±7.73)%]较对照组[(38.09 ±0.48)%]升高;平均移动角度(MAD)低氟组[(68.29 ±5.71)度/s]较对照组[(81.57 ±8.44)度/s]降低;鞭打频率(BCF)高氟组[(11.47 ±0.61)次/s]较对照组[(9.49 ±0.34)次/s]升高;精子密度(p)低、中氟组[(1.26 ±0.24)×10~9、(1.84 ±0.50)×10~9/L]较对照组[(3.94±1.10)×10~9个/L]降低(P均<0.05);曲线速度(VCL)、前向性(STR)、侧摆幅度(ALH),组间比较差异无统计学意义(F值分别为0.264、2.209、1.667,P均>0.05).结论 慢性氟中毒可影响大鼠精子活动度.降低精子密度.损害大鼠生殖系统.
目的 探討慢性氟中毒對大鼠精子活動度的影響,為氟的生殖毒性研究提供實驗依據.方法 健康雄性Wistar大鼠32隻,體質量150～180 g,按體質量隨機分為4組:生理鹽水(對照)組、低、中、高氟組(100、200、300mg·kg~(-1)·d~(-1)NaF),灌胃染毒90 d,每天稱體質量.染毒結束後處死大鼠,摘取肝髒、腎髒、睪汍,稱質量併計算髒器繫數;取附睪遊離精子,WLJY-9000型偉力綵色精子質量檢測繫統測定精子運動參數.結果 染毒第30天低、中氟組大鼠體質量[(235.00±14.56)、(235.44±24.99)g]高于高氟組[(206.00 ±18.16)g,P均<0.05)];第60、90天組大鼠體質量比較差異無統計學意義(F值分彆為0.578、1.893,P均>0.05).大鼠肝髒繫數、腎髒繫數、睪汍繫數組間比較差異無統計學意義(F值分彆為2.148、0.907、1.801,P均>0.05).精子運動參數,平均路徑速度(VAP)高氟組[(25.04 ±4.59)μm/s]較對照組[(20.22 ±3.29)μm/s]升高;直線速度(VSL)低、中、高氟組[(18.82 ±3.19)、(17.84 ±4.54)、(16.46 ±2.63)μm/s]較對照組[(12.48 ±1.73)μm/s]升高;直線性(LIN)低、中、高氟組[(23.84±1.58)%、(24.99±3.37)%、(26.75 ±5.07)%]較對照組[(33.29±4.00)%]降低,襬動性(WOB)中、高氟組[(47.03 ±3.98)%、(49.21±7.73)%]較對照組[(38.09 ±0.48)%]升高;平均移動角度(MAD)低氟組[(68.29 ±5.71)度/s]較對照組[(81.57 ±8.44)度/s]降低;鞭打頻率(BCF)高氟組[(11.47 ±0.61)次/s]較對照組[(9.49 ±0.34)次/s]升高;精子密度(p)低、中氟組[(1.26 ±0.24)×10~9、(1.84 ±0.50)×10~9/L]較對照組[(3.94±1.10)×10~9箇/L]降低(P均<0.05);麯線速度(VCL)、前嚮性(STR)、側襬幅度(ALH),組間比較差異無統計學意義(F值分彆為0.264、2.209、1.667,P均>0.05).結論 慢性氟中毒可影響大鼠精子活動度.降低精子密度.損害大鼠生殖繫統.
목적 탐토만성불중독대대서정자활동도적영향,위불적생식독성연구제공실험의거.방법 건강웅성Wistar대서32지,체질량150～180 g,안체질량수궤분위4조:생리염수(대조)조、저、중、고불조(100、200、300mg·kg~(-1)·d~(-1)NaF),관위염독90 d,매천칭체질량.염독결속후처사대서,적취간장、신장、고환,칭질량병계산장기계수;취부고유리정자,WLJY-9000형위력채색정자질량검측계통측정정자운동삼수.결과 염독제30천저、중불조대서체질량[(235.00±14.56)、(235.44±24.99)g]고우고불조[(206.00 ±18.16)g,P균<0.05)];제60、90천조대서체질량비교차이무통계학의의(F치분별위0.578、1.893,P균>0.05).대서간장계수、신장계수、고환계수조간비교차이무통계학의의(F치분별위2.148、0.907、1.801,P균>0.05).정자운동삼수,평균로경속도(VAP)고불조[(25.04 ±4.59)μm/s]교대조조[(20.22 ±3.29)μm/s]승고;직선속도(VSL)저、중、고불조[(18.82 ±3.19)、(17.84 ±4.54)、(16.46 ±2.63)μm/s]교대조조[(12.48 ±1.73)μm/s]승고;직선성(LIN)저、중、고불조[(23.84±1.58)%、(24.99±3.37)%、(26.75 ±5.07)%]교대조조[(33.29±4.00)%]강저,파동성(WOB)중、고불조[(47.03 ±3.98)%、(49.21±7.73)%]교대조조[(38.09 ±0.48)%]승고;평균이동각도(MAD)저불조[(68.29 ±5.71)도/s]교대조조[(81.57 ±8.44)도/s]강저;편타빈솔(BCF)고불조[(11.47 ±0.61)차/s]교대조조[(9.49 ±0.34)차/s]승고;정자밀도(p)저、중불조[(1.26 ±0.24)×10~9、(1.84 ±0.50)×10~9/L]교대조조[(3.94±1.10)×10~9개/L]강저(P균<0.05);곡선속도(VCL)、전향성(STR)、측파폭도(ALH),조간비교차이무통계학의의(F치분별위0.264、2.209、1.667,P균>0.05).결론 만성불중독가영향대서정자활동도.강저정자밀도.손해대서생식계통.
Objective To probe into the impact on sperm motility in male rat induced by fluorine poisoning, and provide experimental basis to further research for reproductive toxicity of fluoride. Methods According to bodyweight, 32 male Wistar rats were randomly divided into control group, the low-dose, medium-dose and high-dose group( 100,200,300 mg·kg~(-1)·d~(-1) NaF), and were treated by intragastric administration for 90 days, and the weight of the rats was observed each day. After the last intragastric administration, all rats were killed. The relative weight of liver, kidney and testis was calculated. Rat epididymides were plucked off and spermatozoa released from it. Sperm motility parameters were measured by WLJY-9000 color-detection system of sperm quality. Results Compared with high-dose group[(206.00 ± 18.16)g], the weight of low-dose and medium-dose group [ (235.00 ± 14.56), (235.44 ± 24.99)g] in 30 days were statistically significant increased(all P < 0.05) ; there were no significant differences between the groups in 60 days and in 90 days(F = 0.578,1.893, all P > 0.05). Comparison of organ coefficient of liver, kidney and testis among three groups showed no significant difference(F = 2.148,0.907, 1.801, all P > 0.05). The average path velocity(VAP) of the high-dose group[ (25.04 ± 4.59)μm/s] showed significant increase compared with control group[ (20.22 ± 3.29)μm/s] ; the straight line velocity(VSL) of the low- dose, medium-dose and high-dose group[ (18.82± 3.19), (17.84 ± 4.54), (16.46 ± 2.63)μm/s] showed significant increase compared with control group[ ( 12.48 ± 1.73 ) μm/s ] ; linearity (LIN) of the low-dese, medium-dose and high.dose group[(23.84±1.58)%,(24.99±3.37)%,(26.75±5.07)%]showed significant decrease compared with control group[(33.29±4.00)%];wobble(WOB)of the medium-dose and high-dose group[(47.03±3.98)%,(4921±723)%]showed significant increase compared with control group[(38.09±0.48)%];mean angular deviation (MAD)of the low-dose group[(68.29±5.71)radian/s]showed significant decrease compared with control group [(81.57±8.44)radian/s];beat cross frequency(BCF)ofthe high-dose group[(117±0.61)/s]showed significant increase compared with control group[(9.49±0.34)/s];sperm density(p)of the low-dose and medium-dose group [(1.26±0.24)×10~9/L,(1.84±0.50)×10~9/L]showed significant decrease compared with control group [(3.94±1.10)×10~9/L,all P<0.05].Comparison of the eurvilinearvelocity(VCL),straightness(STR),amplitude of lateral head displacement(ALH)among three groups showed no significant difference(F=0.264,2.209,1.667, all P>0.05).Conclusion Fluorine poisoning could change sperm motility parameters of the rat,reduce the sperm density and cau8e damage to the reproductive system.
