国际生物制品学杂志
國際生物製品學雜誌
국제생물제품학잡지
INTERNATIONAL JOURNAL OF BIOLOGICALS
2012年
1期
6-8,20
,共4页
鸡胚%麻疹疫苗%腮腺炎疫苗%疫苗,联合%免疫学技术%逆转录聚合酶链反应
鷄胚%痳疹疫苗%腮腺炎疫苗%疫苗,聯閤%免疫學技術%逆轉錄聚閤酶鏈反應
계배%마진역묘%시선염역묘%역묘,연합%면역학기술%역전록취합매련반응
Chick embryo%Measles vaccine%Mumps vaccine%Vaccines,combined%Immunologic techniques%Reverse transcriptase polymerase chain reaction
目的 建立麻疹腮腺炎减毒活疫苗生产用鸡胚的质量控制方法.方法 运用酶联免疫吸附测定检测禽白血病病毒；运用双向免疫扩散试验检测马立克氏病病毒、网状内皮组织增生症病毒、禽流感病毒、传染性法氏囊病病毒、多杀性巴氏杆菌；运用血凝试验检测血吸附病毒；运用逆转录-聚合酶链反应检测新城疫病毒；依照《中华人民共和国药典》2010年版三部的要求进行无菌检查及支原体和外源因子检查.结果 采用上述方法未在无特定病原体鸡胚(鸡群代码:S、R、D、W、C、F、G、M、H)尿囊液、原代鸡胚成纤维细胞悬液、鸡胚成纤维细胞悬液培养物中检出禽白血病病毒、马立克氏病病毒、网状内皮组织增生症病毒、禽流感病毒、传染性法氏囊病病毒、多杀性巴氏杆菌、血吸附病毒和新城疫病毒,表明采用这些方法对麻疹腮腺炎减毒活疫苗生产用鸡胚进行质量控制是可行的.结论 建立了一套用于麻疹腮腺炎减毒活疫苗生产用鸡胚质量控制的快速、特异的检测方法.
目的 建立痳疹腮腺炎減毒活疫苗生產用鷄胚的質量控製方法.方法 運用酶聯免疫吸附測定檢測禽白血病病毒；運用雙嚮免疫擴散試驗檢測馬立剋氏病病毒、網狀內皮組織增生癥病毒、禽流感病毒、傳染性法氏囊病病毒、多殺性巴氏桿菌；運用血凝試驗檢測血吸附病毒；運用逆轉錄-聚閤酶鏈反應檢測新城疫病毒；依照《中華人民共和國藥典》2010年版三部的要求進行無菌檢查及支原體和外源因子檢查.結果 採用上述方法未在無特定病原體鷄胚(鷄群代碼:S、R、D、W、C、F、G、M、H)尿囊液、原代鷄胚成纖維細胞懸液、鷄胚成纖維細胞懸液培養物中檢齣禽白血病病毒、馬立剋氏病病毒、網狀內皮組織增生癥病毒、禽流感病毒、傳染性法氏囊病病毒、多殺性巴氏桿菌、血吸附病毒和新城疫病毒,錶明採用這些方法對痳疹腮腺炎減毒活疫苗生產用鷄胚進行質量控製是可行的.結論 建立瞭一套用于痳疹腮腺炎減毒活疫苗生產用鷄胚質量控製的快速、特異的檢測方法.
목적 건립마진시선염감독활역묘생산용계배적질량공제방법.방법 운용매련면역흡부측정검측금백혈병병독；운용쌍향면역확산시험검측마립극씨병병독、망상내피조직증생증병독、금류감병독、전염성법씨낭병병독、다살성파씨간균；운용혈응시험검측혈흡부병독；운용역전록-취합매련반응검측신성역병독；의조《중화인민공화국약전》2010년판삼부적요구진행무균검사급지원체화외원인자검사.결과 채용상술방법미재무특정병원체계배(계군대마:S、R、D、W、C、F、G、M、H)뇨낭액、원대계배성섬유세포현액、계배성섬유세포현액배양물중검출금백혈병병독、마립극씨병병독、망상내피조직증생증병독、금류감병독、전염성법씨낭병병독、다살성파씨간균、혈흡부병독화신성역병독,표명채용저사방법대마진시선염감독활역묘생산용계배진행질량공제시가행적.결론 건립료일투용우마진시선염감독활역묘생산용계배질량공제적쾌속、특이적검측방법.
Objective To establish a quality control method for determining chick embryo used for manufacture of measles and mumps combined vaccine.Methods Avian leukosis virus (ALV) was determined by ELISA.Marek's disease virus (MDV),reticuloendotheliosis virus (REV),avian influenza virus (AIV),infectious bursal disease virus (IBD) and Pasteurella multocida were determined by double immunodiffusion test.Hemadsorption virus (HAV) was determined by hemagglutination test.Newcastle disease virus (NDV) was determined by reverse transcription-polymerase chain reaction.Sterility test and tests for mycoplasma and adventitious agents were carried out according to requirements of Pharmacopoeia of the People's Republic of China 2010 Volume Ⅲ.Results No ALV,MDV,REV,AIV,IBD,Pasteurella multocida,HAV and NDV were detected in allantoic fluids of specific pathogen free chick embryo ( the codes of chick group:S,R,D,W,C,F,G,M,H),primary chick embryo fibroblast (CEF) suspensions and CEF cultures by above methods.The results suggested that it was feasible to use these methods for control quality of chick embryo.Conclusions A set of fast and specific methods for quality control of chick embryo used for manufacture of measles and mumps combined vaccine are established.
