CAJ | 학술논문

目的探讨CRKL活性在白血病细胞多药耐药中的作用,发现与耐药相关的新因素.方法应用流式细胞术比较CRKL在K562、HL-60及其耐药细胞中的活性变化;对敏感细胞用柔红霉素处理后,检测CRKL活性与化疗作用时间的关系以及撤药后CRKL活性的改变.结果与K562、HL-60敏感细胞比较,在K562、HL-60耐药细胞株中,CRKL的磷酸化水平明显升高;在用柔红霉素处理72 h后,K562和HL-60细胞CRKL的磷酸化水平与化疗药物作用呈时间依赖性,未发现Jurkat细胞CRKL磷酸化水平的改变.结论 CRKL活性变化与白血病细胞多药耐药的形成有关,是自血病细胞多药耐药的又一个新的影响因素.
목적 탐토CRKL활성재백혈병세포다약내약중적작용,발현여내약상관적신인소.방법 응용류식세포술비교CRKL재K562、HL-60급기내약세포중적활성변화;대민감세포용유홍매소처리후,검측CRKL활성여화료작용시간적관계이급철약후CRKL활성적개변.결과 여K562、HL-60민감세포비교,재K562、HL-60내약세포주중,CRKL적린산화수평명현승고;재용유홍매소처리72 h후,K562화HL-60세포CRKL적린산화수평여화료약물작용정시간의뢰성,미발현Jurkat세포CRKL린산화수평적개변.결론 CRKL활성변화여백혈병세포다약내약적형성유관,시자혈병세포다약내약적우일개신적영향인소.
Objective To investigate the role of CRKL activity in leukemia cells with muhidrug resistance and find new factor related to multidrug resistance. Methods By flow cytometry, CRKL activity was compared in K562, HL-60 cells and its resistance cells. The change of CRKL activity was observed in sensitive cells treated with and withdrawal daunorubicin. Results With the comparison of K562, HL-60 sensitive cells, in K562, HL-60 resistant cell lines, the level of CRKL phosphorylation in K562, HL-60 resistance cells treated with daunombicin 72 hours increased markedly. The level of CRKL phosphorylation was time-dependent with chemotherapy drugs, not change of CRKL activity was found in Jurkat ceils.Conclusion The level of CRKL activity is new factor related to muhidrug resistance in leukemia cells.